Double Immunoenzymatic Labelling (original) (raw)

Immunocytochemistry, 1983

Abstract

Publisher Summary This chapter describes the methods that can be used for labeling pairs of antigens in tissue sections by double immunoenzymatic procedures. The number of antigens that can be detected in human tissue is increasing, principally, as a result of the production of new monoclonal antibodies, and this has led to a growing need for methods that enables the relative distribution patterns of pairs of antigens to be visualized simultaneously in tissue sections. Immunofluorescent techniques have been widely used in the past for double labeling of antigens. However, immunoenzymatic procedures offer several advantages that are likely to result in their being used on an increasingly wide scale in the future. Double immunoenzymatic labeling can be achieved by two fundamentally different approaches, which can be referred to as single-enzyme and double-enzyme methods. In the single-enzyme method, both antigens are labeled using an immunoperoxidase technique, two different enzyme substrates yielding distinctively colored reaction products being used to reveal each antigen.

Zidane Abdulaziz hasn't uploaded this paper.

Let Zidane know you want this paper to be uploaded.

Ask for this paper to be uploaded.