Recent Advances in Biosorption of Copper and Cobalt by Filamentous Fungi (original) (raw)
Related papers
Journal of Environmental Sciences, 2011
Filamentous fungi are able to accumulate significant amount of metals from their environment. The potential of fungal biomass as agents for biosorption of heavy metals from contaminated sediments is currently receiving attention. In the present study, a total of 41 isolates of filamentous fungi obtained from the sediment of the Langat River, Selangor, Malaysia were screened for their tolerance and uptake capability of copper (Cu) and lead (Pb). The isolates were identified as Aspergillus niger, A. fumigatus, Trichoderma asperellum, Penicillium simplicissimum and P. janthinellum. A. niger and P. simplicissimum, were able to survive at 1000 mg/L of Cu(II) concentration on Potato Dextrose Agar (PDA) while for Pb, only A. niger survived at 5000 mg/L concentration. The results showed that A. niger, P. simplicissimum and T. asperellum have a better uptake capacity for Pb compared to Cu and the findings indicated promising biosorption of Cu and Pb by these filamentous fungi from aqueous solution. The present study was also determined the maximum removal of Cu(II) and Pb(II) that was performed by A. niger. The metal removal which occurred at Cu(II) 200 mg/L was (20.910 ± 0.581) mg/g and at 250 mg/L of Pb(II) was (54.046 ± 0.328) mg/g.
Bioresource Technology, 2007
Heavy metal analysis of agricultural field soil receiving long-term (>20 years) application of municipal and industrial wastewater showed two-to five-fold accumulation of certain heavy metals as compared to untreated soil. Metal-resistant fungi isolated from wastewater-treated soil belonged to genera Aspergillus, Penicillium, Alternaria, Geotrichum, Fusarium, Rhizopus, Monilia and Trichoderma. Minimum inhibitory concentrations (MIC) for Cd, Ni, Cr, Cu, and Co were determined. The MIC ranged from 0.2 to 5 mg ml À1 for Cd, followed by Ni (0.1-4 mg ml À1 ), Cr (0.3-7 mg ml À1 ), Cu (0.6-9 mg ml À1 ) and for Co (0.1-5 mg ml À1 ) depending on the isolate.
Biosorption of Copper and Lead by Heavy Metal Resistant Fungal Isolates
Microorganisms play a significant role in bioremediation of heavy metal contaminated soil and wastewater. In this study heavy metal resistant fungi were isolated from the waste water treated soil samples of Hudiara drain, Lahore. The optimum pH and temperature conditions for heavy metal removal were determined for highly tolerant isolates of Aspergillus species along with the initial metal concentration and contact time. Biosorption capacity of Aspergillus flavus and Aspergillus niger was checked against Cu (II) and Pb (II) respectively. The optimal pH was 8-9 for A. flavus and 4-5.4 for A. niger, whereas the optimal temperature was 26°C and 37°C respectively. Moreover, the biosorption capacity of A. flavus was 20.75-93.65 mg/g for Cu (II) with initial concentration 200-1400 ppm. On the other hand the biosorption capacity of A. niger for Pb (II) ranged from 3.25-172.25 mg/g with the same range of initial metal concentration. It was also found that equilibrium was maintained after maximum adsorption.
Biosorption and Bioaccumulation of Copper and Lead by Heavy Metal-Resistant Fungal Isolates
Arabian Journal for Science and Engineering, 2015
Microorganisms play an important role in the bioremediation of heavy metal-contaminated wastewater and soil. In this research, isolation of heavy metal-resistant fungi was carried out from wastewater-treated soil samples of Hudiara drain, Lahore. The purpose of the present investigation was to observe fungal absorption behavior toward heavy metal. The optimum pH and temperature conditions for heavy metal removal were determined for highly tolerant isolates of Aspergillus spp. along with the initial metal concentration and contact time. Biosorption capacity of A. flavus and A. niger was checked against Cu(II) and Pb(II), respectively. The optimal pH was 8-9 for A. flavus and 4-5.4 for A. niger, whereas optimal temperature was 26 and 37 • C, respectively. Moreover, the biosorption capacity of A. flavus was 20.75-93.65 mg g −1 for Cu(II) with initial concentration 200-1400 ppm. On the other hand, biosorption capacity of A. niger for Pb(II) ranged from 3.25 to 172.25 mg g −1 with the same range of initial metal concentration. It was also found that equilibrium was maintained after maximum adsorption. The adsorption data were then fitted to Langmuir model with a coefficient of determination >0.90. The knowledge of the present study will be helpful for further research on the bioremediation of polluted soil.
Fungal Biomass for Cu(II) Uptake from Aqueous Systems
Heavy metal pollution represents a major problem, with a major impact on the environment and life itself . Many wastes and wastewaters contain heavy metals. They result from numerous industries such as: energy and fuel production, electroplating, metal surface treating, surface finishing industry, fertilizer and pesticide industry, electrolysis, leather industry, photography, electro-osmosis, aerospace, atomic energy installation, mining, and smelting. Wastewaters of these industries contain metals classified as:
Environmental Technology & Innovation, 2021
Due to various anthropogenic activities, different heavy metals like arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), mercury (Hg) and nickel (Ni) accumulate in the soils of industrial areas. In the current study, contaminated soil was collected from Yuepu industrial area of Shanghai, China. Soil analysis revealed the presence of Pb and Cu in high concentrations, ranging from 125.9-128.0 mg/kg and 98.4-100.2 mg/kg, respectively. These soil samples were further used to isolate metal tolerant microbes. Subsequent screening resulted in the isolation of four major fungal species including Fusarium fujikuroi, Fusarium solani, Trichoderma citronoviridae and Trichoderma reesei. Molecular characterization of isolated fungi was performed and amplified sequences were deposited in GenBank NCBI database. Metal tolerance and biosorption capacity of these fungal strains towards lead and copper were tested. Fungal strains were exposed to increasing concentrations (100-1000 ppm) of chlorides of lead and copper and the tolerance of the selected fungi was evaluated by measuring the minimum inhibitory concentrations (MIC). The range of MIC values was found to be 400 ppm to 1000 ppm. The tolerance index of all the tested fungi was calculated and T. citronoviridae was observed to be tolerant at 1 mM concentration of lead while F. solani was the most tolerant species at 1 mM concentration of copper. The highest biosorption capacity of Pb was exhibited by T. citronoviridae, while T. reesei showed the best absorption capacity of Cu followed by F. solani. Scanning electron microscopy (SEM) showed visible adsorption of metal on fungal mycelia and suggested it to be the mechanism of metal removal. On the basis of these findings, it could be concluded that T. citronoviridae and F. solani are the potential mycoremediation microbes in Pb and Cu contaminated soils, respectively.
International Journal of Integrative Biology
Physiologically inactive Rhizopus arrhizus biomass adsorbed 26.5 mg of copper from 30 mg/l copper sulphate solution whereas almost equal amount (26.0 mg) of copper was adsorbed by its active form. Results of copper biosorption study by this fungus at different parameters were well fitting with Langmuir and Freundlich isotherm model, proving its efficiency as good copper adsorbent. At optimum pH 5.5 and biomass concentration 0.015 gm/l, it took 90 minutes to remove 97% copper and 80% nickel from their respective solutions where as 95% of copper but 55% of nickel from their combined solution of 30 mg/l strength. However by increasing R. arrhizus biomass concentration from 0.15gm/l to 0.5 gm/l, nickel, and the major contaminant of copper effluent was unaffectedly sequestered as good as 99% in addition to total copper from their combined solution. But for two other fungal species i.e. A. niger and P. chrysogenum as compared to R. arrhizus, complete decontamination of metals from similar effluent solution needed an increased biomass quantity (2.5 gm/l) and in contact time from 90 minutes to 4 hours as well as increased number of treatment cycles from single (R. arrhizus) to multiple cycles (4-5 for two other species). Therefore, among these three fungal species studied, R. arrhizus was found to be the best biosorbent for treating copper industrial effluent especially when nickel is present as major contaminant.
Environmental science and pollution research international, 2018
In this paper, a highly copper-resistant fungal strain NT-1 was characterized by morphological, physiological, biochemical, and molecular biological techniques. Physiological response to Cu(II) stress, effects of environmental factors on Cu(II) biosorption, as well as mechanisms of Cu(II) biosorption by strain NT-1 were also investigated in this study. The results showed that NT-1 belonged to the genus Gibberella, which exhibited high tolerance to both acidic conditions and Cu(II) contamination in the environment. High concentrations of copper stress inhibited the growth of NT-1 to various degrees, leading to the decreases in mycelial biomass and colony diameter, as well as changes in morphology. Under optimal conditions (initial copper concentration: 200 mg L, temperature 28 °C, pH 5.0, and inoculum dose 10%), the maximum copper removal percentage from solution through culture of strain NT-1 within 5 days reached up to 45.5%. The biosorption of Cu(II) by NT-1 conformed to quasi-sec...
Fungal biosorption -- an alternative treatment option for heavy metal bearing wastewaters: a review
Bioresource Technology, 1995
The common jilamentous fungi can sorb heavy metals from aqueous solutions, The sorption of heavy metals, Cu, Zn, Cd, Pb, Fe, Ni, Ag, Th, Ra and u by fungal biomass has been observed to varying extents. Fungal biosolption largely depends on parameters such as pH, metal ion and biomass concentration, physical or chemical pre-treatment of biomass, presence of van'ous ligands in solution, and to a limited extent on temperature. Fungal biosorption pegorrns well in comparison to sorption on commercial ion-exchange resins, activated carbon, and metal oxides. Limited data indicate the potential for regenerating the biomass. The cell-wall fraction of biomass plays an important role in the sorption of heavy metals. The mechanisms of biosorption are understood only to a limited extent. The potential of fungal biomass as sorbents is indicated by the available data, and more research and development of the fungal biosorption technology is recommended.
This study investigated the effect of Al, Fe(II) and Mn ions on growth, accumulation and sorption potential of tolerant fungi from polluted environment. Isolates were screened for metal resistance by plate diffusion method and tolerance ability observed in Sabouraud dextrose broth. Tolerant fungi were pre accumulation and sorption capacity. fumigatus, A. niger, T. viride, Penicillium sp. and T. longibrachiatum molecularly characterized as asperellum BHU216 were tolerant to 300 mg significant inhibition zones and no growth at low metal concentrations. Growth of tolerant fungi was optimum at 24showed iron(II) oxidation expressed by colour change in medium. Enhanced accumulation of metals from < 40% to >60% was achieved when tolerant fungi were pre treated with ethanol in a three high metals' removal with increased metal concentration until saturation (>1.5 mg 70 mg.L -1 ). Results therefore showed that both tolerant fungi strains can act as effective biosorbent with further optimization.