Differential antennal sensitivities of the generalist butterflies Papilio glaucus and P. canadensis to host plant and non-host plant extracts (original) (raw)

Olfaction in phytophagous ladybird beetles: antennal sensilla and sensitivity to volatiles from host plants in Chnootriba elaterii

Arthropod-Plant Interactions

Olfaction and chemical ecology in phytophagous ladybird beetles have been largely ignored so far. The present paper describes for the first time basiconic, grooved, and trichoid porous olfactory sensilla on the antennae of the phytophagous ladybird Chnootriba elaterii (Epilacnini) and demonstrates the sensitivity of the antennae to Volatile Organic Compounds (VOCs) from host plants, belonging to common and less common Cucurbitaceae crops and wild species (Citrullus lanatus, Cucumis melo, Cucumis sativus, Cucurbita pepo, Cucurbita moschata, Lagenaria siceraria, Luffa cylindrica, and Ecballium elaterium). Epilachnini ladybirds are phytophagous, with adult and larvae feeding on various Cucurbitaceae, Fabaceae, and Solanaceae. Notwithstanding some species are serious pests in crops, their chemical ecology has been largely disregarded so far. The present paper discusses the ultrastructure of antennal sensilla (scanning electron microscopy-SEM) and the electroantennographic recordings (EA...

Antennal electrophysiological responses of the giant swallowtail butterfly, Papilio cresphontes, to the essential oils of Zanthoxylum clava-herculis and related plants

We used the electroantennogram (EAG) technique to compare the antennal sensitivity of both sexes of the giant swallowtail butterfly, Papilio cresphontes to four doses (1, 10, 100, and 1,000 lg) of the leaf essential oils of Zanthoxylum clava-herculis and Ptelea trifoliata (key host plants) and Sassafras albidum (a marginal or non-host plant). The main hypothesis tested was that P. cresphontes will show greater olfactory sensitivity to volatiles of the key host plants than to volatiles of the marginal host plant, in particular at low doses. At the lower doses, extract of the key host plant, Z. clava-herculis elicited greater EAG responses in both sexes than extracts of the remaining two plants. At higher doses, however, extracts of P. trifoliata and S. albidum elicited greater EAG responses than extract of Z. clava-herculis. These results partly support our hypothesis and may suggest that Z. clava-herculis is a more preferred host plant of P. cresphontes than P. trifoliata. In general, female butterflies showed greater EAG responses than males to the three plant extracts at the higher doses. Preliminary coupled gas chromatography electroantennogram (GC-EAD) tests revealed four components each from Z. clava-herculis and P. trifoliata (three peaks common to both extracts) and seven from S. albidum (one shared with Z. clava-herculis) which elicited GC-EAD activity in P. cresphontes females, but the peaks were un-indentified because most were detected in traceamounts. In addition, the chemical composition of the leaf essential oil of Z. clava-herculis was analyzed by GC–MS. The leaf essential oils consisted of 25 components, largely menthane monoterpenoids, dominated by limonene and 1,8-cineole, but neither of the two major components elicited significant GC-EAD response in P. cresphontes. These results are discussed in relation to host-plant selection in P. cresphontes.

Use of volatiles of Aristolochia chilensis (Aristolochiaceae) in host searching by fourth-instar larvae and adults of Battus polydamas archidamas (Lepidoptera: Papilionidae: Troidini)

Papilionid butterflies of the tribe Troidini are specialists on plants of the family Aristolochiaceae. The role of plant volatiles in host recognition by adult and larval stages of these insects remains unknown. We used Battus polydamas archidamas (Papilionidae: Troidini) and its host-plant, Aristolochia chilensis (Aristolochiaceae), to study: (i) the olfactory and electrophysiological responses of adults to headspace volatiles of the host-plant, (ii) the chemical composition of the headspace volatiles of the host-plant, (iii) the patterns of aggregation of larvae in the field in order to ascertain the time when they leave the plant where the eggs were laid, and (iv) the olfactory responses of solitary-feeding fourth-instar larvae to headspace volatiles of the host-plant. Larvae left their initial host-plant during the third or fourth instar. Host-plant headspace volatiles attracted fourth-instar larvae as well as adults; adult females were more responsive than males. Taken together, these results reveal changes in the responsiveness to host-plant volatiles during development, and provide an insight into the host-plant specialization of this butterfly.