Multidrug Resistance and High Prevalence of class 1 Integrons in Escherichia coli Isolated from Irrigation Water and Vegetables in Parts of Nsukka and Enugu, Nigeria (original) (raw)
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Feco-orally transmitted infectious diseases are common in Nigeria where the potable water access is poor. In the south-western Nigerian Ibadan metropolis, supply of municipal water is meagre as residents depend on household wells and boreholes. The likelihood of fecal contamination of household water sources in Ibadan was examined longitudinally to quantify and understand its impact. Well and borehole water samples aseptically collected from 96 households in Ibadan were assessed for total heterotrophic counts (THCs), total coliform counts (TCCs) and total Escherichia coli counts (TECs) using a pour plate technique. E. coli were identified by uidA and whole-genome sequencing using Illumina technology, whereas virulence factors were predicted using VirulenceFinder. There was season-independent abundance of THC and TCC in the well and borehole with a significant recovery of E. coli in the wells during the wet season compared to the dry season (P = 0.0001). Virulence genes associated wi...
International Journal of Environmental Research and Public Health
Introduction: Safety of the environment in which vegetables are grown, marketed and consumed is paramount as most are eaten raw. Irrigation sources include open drains and streams, which are often contaminated with human and animal waste due to poor sanitation infrastructure. In irrigated vegetable farms using such sources in Ghana, we assessed Escherichia coli counts, antibiotic resistance patterns and resistant genes on irrigated lettuce. Methods: A cross-sectional study was conducted between January–May 2022, involving five major vegetable farms in Ghana. Results: Escherichia coli was found in all 25 composite lettuce samples analyzed. Counts expressed in CFU/g ranged from 186 to 3000, with the highest counts found in lettuce irrigated from open drains (1670) and tap water using hose pipes (3000). Among all bacterial isolates, resistance ranged between 49% and 70% for the Watch group of antibiotics, 59% for the Reserved group and 82% were multidrug-resistant. Of 125 isolates, 60 ...
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Prevalence of multidrug-resistant Escherichia coli in household drinking water in rural Ghana
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Even in the 21st century, households in rural Ghana still rely on drinking water sources that are contaminated with pathogenic Escherichia coli, the consumption of which results in morbidity and mortality of children and adults. The present study sought to determine the prevalence and antimicrobial susceptibility profile of E. coli isolated from household drinking water. A total of 49 water samples were analyzed. E. coli was isolated and confirmed from the water samples using the spread plate and biochemical tests, respectively. The Kirby–Bauer method was used for antimicrobial susceptibility testing. E. coli was isolated from 79.6% of the water samples with a mean colony growth of 15 cfu/100 μl. The isolates were absolutely resistant to ceftazidime, cefixime, augmentin, and cefuroxime. In contrast, the isolates were most susceptible to ciprofloxacin, ofloxacin, gentamicin, and nitrofurantoin. Multidrug resistance was registered in 48.7% of the isolates. E. coli isolates from each w...
Escherichia coli contamination of drinking water source has continued to emerge, as an important public health concern across the globe. This had been associated with water born epidemic outbreak, especially in rural communities were access to potable water remains a massive challenge. However, identifying the source of Escherichia coli pollution in public water source had been hugely attributed to faecal contamination either from man or animals which constitute a serious environmental and public health threat to man. This study was designed and thus, aimed to determine the bacteriological quality of borehole water sources with specific reference to E. coli contamination in the studied locations (in Mile 2 and 3 area of Diobu, Port Harcourt).However, A convenience random sampling research design was explored to collect 50 water samples aseptically, and it was analyzed, using standard microbiological cultural technique (All samples were cultured on MacConkey and Nutrient agar plates respectively). The positive samples were examined for faecal coli form. Identification of Escherichia coli was done based on microscopic, cultural and biochemical characteristics. Of the 50 samples collected and processed for coli form presence, 22 samples (44%) showed growth of Escherichia coli when cultured on nutrient agar, 15 samples (30%) showed growth of Escherichia coli when cultural on MacConkey agar while 13 (26%) showed no growth on both media. This result strongly suggest that 74% of the boreholes studied did not meet up with the World Health Organization Standards guideline for drinking water source, thus will certainly pose a public health risk to the inhabitant of the area if left untreated. Nonetheless, , it is firmly recommendation that borehole water from these affected areas should be treated, boiled and filtered before use for drinking and washing of fresh fruits to avert the possibilities of an outbreak of the water borne epidemic in the area.