Continuous column culture system for adherent cells (original) (raw)

Cell Culture: History, Development and Prospects

Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

The Bulk Growth of Animal Cells in Continuous Suspension Culture

Journal of General Microbiology, 1959

Several methods for growing animal cells in suspension culture were examined, to find the most efficient in terms of cells produced in a given time for the minimum of medium and attention. Continuous medium flow was more efficient than batch-culture, and the preferred system was to add medium to a culture vessel regularly in small doses via a time-switch-controlled solenoid closure a t a rate similar to the growth rate; a turbidimetric safety device ensured that cell density did not drop below levels permitting growth. A mixture of galactose or fructose (6 g./l.) and glucose (2 g./l.) allowed better pH control than glucose alone (6 g./l.). Doubling times of 14-16 hr. were obtained on occasion; the gas-phase oxygen concentration for fastest growth depended on cell density and was frequently less than atmospheric, suggesting that these cells can behave as microaerophils. ' maximal ' medium (medium CSV.6, Table 1) giving fastest growth and highest

Cell And Tissue Culture Laboratory Manual

Cell And Tissue Culture Laboratory Manual by: Dr. Tarek Yehia Kapiel B.Sc., M.Sc., Ph.D. This practical course (BT 202) was provided to Biotechnology Students, Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA). http://www.msa.eun.eg