In-Vitro Antibacterial Activity of Crude Ethanol and Aqueous Garcinia Kola Seed Extracts on Selected Bacterial Isolates (original) (raw)

The study was conducted to screen in-vitro antibacterial activity of crude ethanol and aqueous extracts of Garcinia kola seed against some selected bacteri al solates composing of Gram positive and Gram negative organi sms namely Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The disc diffusion method was employed to determine the inhibitory effects of the seed extract on the test micro-organisms. The ethanol and aqueous extract of the seed at concentration of 4g/ 40ml were diluted at the concentration of 0.1g/ml, O.2g/ml, 0 .4g/ml, 0.6g/ml and 0.8g/ml, then impregnated into a steril e filter paper disc. Also ethanol alone was impregnated into the sterile filter paper disc and used as control. The zones of inhibition obtained for ethanol extract (4g/40ml) of Garcinia kola seed ranged between 12mm-15mm. The zone of inhibition obtained for diluted ethanol extract (4g/40ml) of Garcinia kola seed at concentration 0.1g/ml, 0.2g/m l, 0.4g/ml, 0.6g/ml and 0.8glml range...