The quantity of neutrofil and makrofag after the application of red ginger on white rats (Ridha Andayani et al.) The quantity of neutrophil and macrophage after the application of red ginger on white rats with chronic periodontitis (original) (raw)
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Padjadjaran Journal of Dentistry
Introduction: Chronic periodontitis is the most common form of periodontitis. The major etiology of periodontitis is gram-negative anaerobic bacteria, such as Aggregatibacter actinomycetemcomitans, Treponema denticola, Tannarela forsythia and Porphyromonas gingivalis which locally invade periodontal tissues and affect the immune system cells. Neutrophils and macrophage play central role in inflammation. The objective of this study was to evaluate the quantity of neutrophil and macrophage due to the application of red ginger (Zingiber officinale var rubrum) extract of rat chronic periodontitis. Methods: The type of this research was experimental laboratoris post test only control group. In order to induce experimental periodontitis, the mandibular insisivus was assigned to receive wire 0,008 inch in marginal gingival. Then, red ginger (Zingiber officinale var rubrum) extract that has bioactive compounds was topically administered to the rats. Results: This study showed there were no ...
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 2019
Background: The aim of this study was to evaluate the effects of ginger supplementation on inflammatory, antioxidant, and periodontal parameters in type 2 diabetes mellitus (T2DM) patients with chronic periodontitis (CP) under non-surgical periodontal therapy (NSPT). Material and methods: In this double-blind clinical trial study, 46 T2DM patients with CP were randomly allocated to intervention and control groups and received either 4 tablets 500 mg (2 g) ginger or placebo twice a day for 8 weeks. All patients were treated with NSPT during the intervention period. Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), hs-C-reactive protein (hs-CRP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), periodontal indices including clinical attachment loss (CAL), bleeding on probing (BOP), pocket depth (PD), and plaque index were evaluated in all subjects pre-and postintervention. Results: Following 8 weeks of ginger treatment with NSPT, significant reductions were observed in the mean levels of IL-6 (p=0.001), hs-CRP (p=0.03), TNF-α (p=0.007), CAL, and PD (p<0.001) in the intervention group. The mean serum levels of SOD and GPx were significantly increased in the intervention group after the intervention (p=0.001 and 0.002, respectively). At the end of the study, the mean changes of GPx were significantly higher in the intervention group compared with the control group (p=0.04). Also, after the administration of the ginger with NSPT, significant decrease occurred in the mean change of IL-6 (p=0.009), hs-CRP (p=0.049), TNF-α (p=0.049), CAL (p=0.003), and PD (p=0.04) compared with the control group. Conclusion: It is recommended that ginger supplementation along with NSPT may be effective in the improvement of inflammation, oxidative, and periodontal status in T2DM with CP.
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 2019
Background: The aim of this study was to evaluate the effects of ginger supplementation on inflammatory, antioxidant, and periodontal parameters in type 2 diabetes mellitus (T2DM) patients with chronic periodontitis (CP) under non-surgical periodontal therapy (NSPT). Material and methods: In this double-blind clinical trial study, 46 T2DM patients with CP were randomly allocated to intervention and control groups and received either 4 tablets 500 mg (2 g) ginger or placebo twice a day for 8 weeks. All patients were treated with NSPT during the intervention period. Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), hs-C-reactive protein (hs-CRP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), periodontal indices including clinical attachment loss (CAL), bleeding on probing (BOP), pocket depth (PD), and plaque index were evaluated in all subjects pre-and postintervention. Results: Following 8 weeks of ginger treatment with NSPT, significant reductions were observed in the mean levels of IL-6 (p=0.001), hs-CRP (p=0.03), TNF-α (p=0.007), CAL, and PD (p<0.001) in the intervention group. The mean serum levels of SOD and GPx were significantly increased in the intervention group after the intervention (p=0.001 and 0.002, respectively). At the end of the study, the mean changes of GPx were significantly higher in the intervention group compared with the control group (p=0.04). Also, after the administration of the ginger with NSPT, significant decrease occurred in the mean change of IL-6 (p=0.009), hs-CRP (p=0.049), TNF-α (p=0.049), CAL (p=0.003), and PD (p=0.04) compared with the control group. Conclusion: It is recommended that ginger supplementation along with NSPT may be effective in the improvement of inflammation, oxidative, and periodontal status in T2DM with CP.
Dentistry Journal
Objective: To distinguish clinical effects and mechanisms of sodium monofluorophosphate plus xylitol and herbal extracts of Swiss medicinal plants (Chamomilla recutita, Arnica montana, Echinacea purpurea, and Salvia officinalis). Materials and Methods: A 2-month-long comparative clinical study of toothpaste containing 1450 ppm sodium monofluorophosphate and xylitol (control, 15 patients) and toothpaste additionally containing extracts of the medicinal herbs (experiment, 35 patients) was performed on patients with gingivitis and the initial stage of periodontitis. Clinical indices of gingivitis/periodontitis were quantified by Loe & Silness’s, CPITN, OHI-S, and PMA indexes. The pro-inflammatory and anti-inflammatory interleukins, nitrites/nitrates, total antioxidant activity, and bacterial pattern characteristic for gingivitis and periodontitis were quantified in the gingival crevicular fluid and plaque. In the in vitro tests, direct anti-bacterial effects, inhibition of catalase ind...
Sağlık Bilimlerinde Değer
Aim: The aim of this study was to evaluate the effects of systemically-administered Coriandrum sativum L (CSL) and low dose doxycycline (LDD) on serum levels of antioxidant enzymes and intensity of inflammatory cells in rats with experimental periodontitis. Material and Methods: Forty adult male Wistar Albino rats were divided randomly into 5 groups as follows: group 1: periodontally healthy (control); group 2: periodontitis; group 3: periodontitis+CSL (32mg/kg); group 4: periodontitis+CSL (200mg/kg); group 5: periodontitis+LDD (6 mg/kg). Serum gingival superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) levels were evaluated by ELISA. The intensity of inflammatory cells were evaluated by histopathologically. Results: SOD levels were statistically lowest in group 1 and statistically were highest in group 2 than those of other groups. There was a statistical difference in SOD levels in paired comparisons of groups 1 and 2 with other groups. Its level did not h...
Neutrophil dysfunction in rats with natural gingivitis
Archives of Oral Biology, 1993
The functions of polymorphonuclear neutrophils (PMN) from SUS rats with naturally occurring gingivitis were examined by the luminol-dependent chemiluminescence (CL), adherence and bactericidal tests. SUS rats with pre-gingivitis showed lower CL responses of isolated PMNs and whole blood than control rats (RES rats). After plague formation and progression of gingivitis, the CL response gradually increased in SUS rats. RES rats had healthy gingiva and showed no increase in CL responses. Impaired PMN adherence was observed in SUS rats with pre-gingivitis but not in RES rats. PMNs from SUS rats with pre-gingivitis also showed lower bactericidal activity than those from RES rats. Dysfunction of PMNs might induce gingivitis as a result of decreased protection against periodontal pathogens and an elevated level of CL response can be recognized with progression of gingivitis.
Majalah Kedokteran Gigi Indonesia
Periodontitis is a periodontal disease involving the gingiva, periodontal ligament, cementum, and alveolar bone due to an inflammatory process. Virulence factors of periodontopathogens and inflammatory responses in periodontitis can stimulate nuclear factor-kappa B (NF-κB) activity. Charantoside c and Momordicosides g in bitter gourd prevent NF-κB activation stimulated by TNF-α in HepG2 cells. This study aims to determine the effect of bitter gourd fruit (Momordica charantia) ethanolic extract as an anti-inflammatory substance on the level of inflammatory infiltrates and the number of cells that experience NF-κB activation in the periodontitis model. Eighty male Wistar rats were divided into 5 groups. The mandibular incisors were ligated for 14 days to induce periodontitis. Each group was given Momordica charantia extract of 500 mg/kg BW, 250 mg/kg BW, and 100 mg/kg BW; ibuprofen 100 mg/kg BW; and aquades orally using oral gavage on the day 14. Rat necropsy was carried out on day 1,...
Russian Open Medical Journal, 2021
Objective — to study the effectiveness of topical application of standardized fermented papaya gel (SFPG) in the complex therapy of moderate chronic periodontitis developed as an orthodontic treatment complication. Material and Methods — Our study involved 50 patients with periodontitis of a moderate severity developed against the background of a treatment with a fixed orthodontic appliance. Periodontitis has developed as a long-term consequence of the fixed orthodontic appliance. Orthodontic treatment of patients was carried out with metal ligature braces. The patients were divided into two groups: Group 1 with conventional periodontitis treatment supplemented by SFPG applications on the gums for 15 minutes daily during 14 days; Group 2 with conventional treatment alone. The concentration of proinflammatory and anti-inflammatory cytokines in periodontal pockets was determined using solid-phase type of enzyme immunoassay (Vector-Best test system, Russia). The nitrite/nitrate content...
A review of rat models of periodontitis treated with natural extracts
Objective: To review treatment methods using natural extracts applied in rat models of periodontitis to establish a direction for the design of future experiments. Methods: An electronic search of PubMed was carried out using the keywords "periodontitis," "natural", "extracts", "herb*", "plants" and "rats." Articles were screened against inclusion and exclusion criteria by two independent researchers. Data describing the characteristics of rats, method of periodontitis inducement, extract administration, and outcome measures were extracted and analyzed by more than two authors manually. Results: Of the 864 articles identified, 33 studies were included. The use of SpragueeDawley rats (51.2%) and male rats (90.9%) was preferred. The most common experimental methods were ligature placement (72.7%) and oral administration (66.7%). Alveolar bone loss was evaluated mainly by photography (51.5%) and micro-computed tomography (39.4%). Factors related to bone remodeling and inflammatory processes, such as interleukin-1b, tumor necrosis factor-a, receptor activator of nuclear factor-kB, and osteoprotegerin, were also measured. Conclusion: Many diverse experimental periodontitis models have been used. However, few articles observed bone formation, immune responses, antibacterial effects, and toxicity. Future studies to assess natural extracts for the treatment of periodontitis should be robust and well-designed.