Bacterial biofilms formed in arsenic-containing water: biodeterioration of water network materials (original) (raw)
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BioMed Research International, 2019
High levels of arsenic present in the High Altitude Andean Lakes (HAALs) ecosystems selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms potentially useful in bioremediation processes. We herein performed a detailed characterization of the arsenic tolerance profiles and the biofilm production of two HAAL polyextremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass and polypropylene surfaces were evaluated together with the effect of increasing doses and oxidative states of arsenic over the quality and quantity of their biofilm production. The arsenic tolerance outcomes showed that HAAL strains could tolerate higher arsenic concentrations than phylogenetic related strains belonging to the German collection of microorganisms and cell cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ), which suggest adaptations of HAAL strains to their original environment. ...
Mutual interaction between arsenic and biofilm in a mining impacted river
Science of The Total Environment
• Epilithic biofilms growing in a goldmining impacted river accumulate high As concentrations. • Arsenic inhibits algal growth and increases bacterial and dead diatom densities. • Methylated As-species found intracellularly suggests As-detoxification by biofilms, even under eutrophic conditions. • Nutrients, DOC, temperature or light availability must be considered when analyzing effects of As in freshwater ecosystems.
Journal of Basic Microbiology, 2008
The present paper compares the arsenic removal capacities of three bacterial strains namely, Ralstonia eutropha MTCC 2487, Pseudomonas putida MTCC 1194 and Bacillus indicus MTCC 4374 form wastewater (simulated acid mine drainage) containing arsenic (As(III) : As(V) :: 1 : 1), Fe, Mn, Cu and Zn in the concentration of 15 mg/l, 10 mg/l, 2 mg/l, 5 mg/l and 10 mg/l respectively, in bulk liquid phase. Growth patterns of these bacteria in presence of arsenic in solution as well as under starvation have also been investigated as the acid mine drainage normally does not contain organic carbon and also contains high arsenic. At the nutrient broth concentration of 1.25 g/l and in presence of 15 mg/l arsenic sufficient growth of these strains have been observed. However, growth of Ralstonia eutropha MTCC 2487 has been found slightly more than Pseudomonas putida MTCC 1194 and Bacillus indicus MTCC 4374. Arsenic removal capacities of Ralstonia eutropha MTCC 2487, Pseudomonas putida MTCC 1194 and Bacillus indicus MTCC 4374 from simulated acid mine drainage are ~67%, 60% and 61% respectively. It has also been observed that arsenic concentration of 15 mg/l prolongs the stationary phase of these strains. pH and temperature for the above studies have been maintained at 7.1 ± 0.1 and 29 ± 1 °C, respectively.
Journal of environmental management, 2018
Health of millions of people is threatened by the risk of drinking arsenic-contaminated water worldwide. Arsenic naturally conflicts with the concept of life, but recent studies showed that some microorganisms use toxic minerals as the source of energy. Hence, the researchers should consider the development of cost-effective and highly productive procedures to remove arsenic. The current study was conducted on a native bacterial population of Seyed-Jalaleddin Spring Kurdistan, Iran. Accordingly, the arsenic amount in water samples was measured >500 μg/L by the two field and in vitro methods. Water samples were transferred to laboratory and cultured on chemically defined medium (CDM) with arsenic salts. A total of 14 native arsenic-resistant bacterial strains were isolated and after providing pure culture and performing biochemical tests, the isolates were identified using polymerase chain reaction (PCR) and 16s rRNA genomic sequencing. The potential of bacterial strains for the b...
Arsenic resistance and removal by marine and non-marine bacteria
Journal of Biotechnology, 2007
Arsenic resistance and removal was evaluated in nine bacterial strains of marine and non-marine origins. Of the strains tested, Marinomonas communis exhibited the second-highest arsenic resistance with median effective concentration (EC 50) value of 510 mg As l −1 , and was capable of removing arsenic from culture medium amended with arsenate. Arsenic accumulation in cells amounted to 2290 g As g −1 (dry weight) when incubated on medium containing 5 mg As l −1 of arsenate. More than half of the arsenic removed was related to metabolic activity: 45% of the arsenic was incorporated into the cytosol fraction and 10% was found in the lipid-bound fraction of the membrane, with the remaining arsenic considered to be adsorbed onto the cell surface. Potential arsenic resistance and removal were also examined in six marine and non-marine environmental water samples. Of the total bacterial colony counts, 28-100% of bacteria showed arsenic resistance. Some of the bacterial consortia, especially those from seawater enriched with arsenate, exhibited higher accumulated levels of arsenic than M. communis under the same condition. These results showed that arsenic resistant and/or accumulating bacteria are widespread in the aquatic environment, and that arsenic-accumulating bacteria such as M. communis are potential candidates for bioremediation of arsenic contaminated water.
Scientific Reports, 2017
Representative biomarkers (e.g., n-alkanes), diversity and microbial community in the aquifers contaminated by high concentration of arsenic (As) in different sediment depth (0-30 m) in Jianghan Plain, Hubei, China, were analyzed to investigate the potential mechanism of As enrichment in groundwater. The concentration of As was abundant in top soil and sand, but not in clay. The analysis of the distribution of n-alkanes, CPI values, and wax to total n-alkane ratio (Wax(n)%) indicated that the organic matter (OM) from fresh terrestrial plants were abundant in the shallow sediment. However, n-alkanes have suffered from significant biodegradation from the depth of 16 m to 30 m. The deposition of fresh terrestrial derived organic matters may facilitate the release of As from sediment to groundwater in the sediment of 0-16 m. However, the petroleum derived organic matters may do the favor to the release of As in the deeper section of borehole (16 m to 30 m). The 16S rRNA gene sequences identification indicated that Acidobacteria, Actinomycetes and Hydrogenophaga are abundant in the sediments with high arsenic. Therefore, microbes and organic matters from different sources may play important roles in arsenic mobilization in the aquifers of the study area. Arsenic (As) contamination of groundwater is a problem that affects millions of people across the world 1 , which is related to arsenic poisoning, such as heart disease, cancer, stroke, chronic lower respiratory diseases, and diabetes 2,3. Previous studies on the aquifers contaminated by high level of arsenic have indicated that the major source of As in groundwater is released from the sediment 4-8. As resistant microbes interacting with various geochemical processes, play an important role for the mobilization and transformation of As in aquifers and groundwater 9,10. Arsenic may be mobilized from arsenic bearing iron oxides/hydroxides within aquifer sediments into groundwater, as organic matter (OM), especially for labile redox-active components of the OM, e.g., humic substances, plays a critical role in increasing rates of microbial-mediated iron reduction and ground arsenic mobilization 11,12 Therefore, study the characteristics of organic matters (OM) is very important for understanding the mechanism of arsenic mobilization in arsenic-contaminated aquifers. Biomarker analysis can provide useful clues about microbial activity in aquifer sediments 13,14. The mechanism for arsenic-transforming microbes facilitating the release and mobilization of arsenic from aquifers to groundwater have been reported previously 15,16. For example, Pseudomonas species and Clostridium species, as the effective metal reducing microbes, were identified from the sediments in Bengal delta, where high arsenic was detected 17. The relative abundances of different bacteria (such as, Deltaproteobacteria, namely Geobacter species, and Taxa), have been reported to correlate with iron reduction and arsenic mobilization 15,18,19. Except for organic matter quality and quantity, and the concentrations of specific metals affecting the bacterial community structure in high arsenic aquifers 19 , bacterial community structure may be specific in different environmental conditions 20 .
Biochimie, 2009
Arsenic is responsible for the contamination of water supplies in various parts of the world and poses a major risk to human health. Its toxicity and bioavailability depend on its speciation, which in turn, depends on microbial transformations, including reduction, oxidation and methylation. This review describes the development of bioprocesses for the treatment of arsenic-contaminated waters based on bacterial metabolism and biogeochemical cycling of arsenic.