Vaccine potentiality of different antigenic preparations of Aeromonas hydrophila in Rohu, Labeo rohita Ham (original) (raw)
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Immune responses and protection in rohu vaccinated against Aeromonas hydrophila infection
Aquaculture International, 2014
In this study, efficacy of three antigenic preparations from the fish pathogen Aeromonas hydrophila was evaluated as vaccine candidate in rohu Labeo rohita. The rabbit anti-rohu globulin conjugate was prepared in rabbit using sera obtained from healthy adult rohu. Three antigens, namely formalin inactivated A. hydrophila (FAH), FAH mixed with Freund's incomplete adjuvant in a 1:1 (v/v) ratio (FAH ? A), and extra cellular product were prepared. Protection of rohu against A. hydrophila infection was tested at days 10, 20, and 30 post-vaccination. At the end of the vaccination, fish were challenged with A. hydrophila (dose: 1 9 10 7 cfu ml-1) and relative per cent of survival was recorded up to 60th day of post-challenge. Study of cellular immunological parameters including antigen-specific leucocyte proliferation, antigen-specific nitric oxide production, and superoxide anion production exhibited significantly higher (p \ 0.05) values on 10th and 20th day of post-vaccination compared with control. Among all the antigenic groups, FAH ? A showed most significant responses compared with the other groups and produced maximum antibody on 30th day following vaccination. Among the humoral immune responses, lysozyme activity showed almost similar trend like cellular parameters. The result of the challenge study showed a higher level of survival in all the vaccinated groups, especially in FAH ? A group. Our results suggest that vaccine FAH ? A can effectively protect rohu against A. hydrophila infection and could offer an appropriate strategy to prevent this infection in rohu farms. Shib Sankar Sen and Sib Sankar Giri have contributed equally in this work.
Environment & Ecology, 2010
Indian major carp, Labeo rohita immunized with killed Aeromonas hydrophila vaccine showed an antibody peak on day 15 after priming and first booster. A quicker antibody peak was attained only after second booster on day 7. Chloramphenol (CAP) treatment did not affect the kinetics of antibody production, however peak antibody titres were lowered by 1-2 folds. After 30 days of immunization the antibody level in the CAP treated group and the control group stabilized at the same level indicating that CAP treatment did not have long term effect on secondary immune response and memory formation in L. rohita.
Veterinary research communications, 2011
Specific and non-specific immune response to different doses of formalin killed whole cell vaccine of Aeromonas hydrophila to Indian major carp (Labeo rohita) was evaluated in laboratory condition. Three different doses (10(5) CFU/ml, 10(7) CFU/ml, 10(10) CFU/ml) were administered (0.2 ml/fish) intraperitoneally for 1 month. Among the three doses, 10(10) CFU/ml elicited the highest antibody and protective response followed by the doses 10(7) CFU/ml and 10(5) CFU/ml. Upon challenge with the virulent strain of A. hydrophila, the relative percentage of survival was recorded up to 80% at highest dose of 10(10) CFU/ml. The non-specific responses, similar to the specific immune responses were also maximum at highest dose of 10(10) CFU/ml. Similar to the specific immune responses, the non-specific responses were maximum at highest dose of 10(10) CFU/ml. Therefore, dose containing 10(10) CFU/ml of formalin killed cells was found to be the most effective dose for vaccination which increased ...
Aeromonas hydrophila is one of the serious pathogens in ornamental fishes causing hemorrhagic bacterial septicemia leading to inflammation and necrosis of the gastrointestinal tract, kidney, muscle, and spleen. Recent studies demonstrated that immunoproteomic vaccines provide protection against bacterial pathogens in fin fish aquaculture and the vaccines are popular due to its long lasting immunity, safety and low cost versatile characteristics. In this study we used an Outer Membrane Protein (OMP) of Aeromonas hydrophila as a vaccine to provide protection against the pathogen in goldfish (Carassius auratus). We used the extract of Asparagus racemosus as an adjuvant in the vaccine preparation. Surivival and immunological response of the vaccinated fishes (30 and 60 days post vaccination (dpv)), were evaluated after challenge with virulent A. hydrophila. The vaccine treated experimental groups significantly improved (P<0.05) the survival at 50% compared to the controls and had improved immunological responses including phagocytosis, albumin-globulin ratio, serum bactericidal activity, and serum lysozyme activity. Volume 4(2): 045-049 (2012) -047 J Microbial Biochem Technol ISSN:1948-5948 JMBT, an open access journal Citation: Thangaviji V, Michaelbabu M, Anand SB, Gunasekaran P, Citarasu T (2012) Immunization with the Aeromonas OMP Provides Protection against Aeromonas hydrophila in Goldfish (Carassius auratus). J Microbial Biochem Technol 4: 045-049.
Veterinary Immunology and Immunopathology, 2012
Aeromonas hydrophila is an important fish pathogen responsible for huge economic losses in aquaculture sector. The bacterial outer membrane proteins (OMPs), especially adhesins play a key role in the virulence of the bacteria and are considered potential vaccine candidates. We evaluated the immunogenicity of two important outer membrane proteins namely Aha1 and OmpW of A. hydrophila. These proteins were over-expressed in Escherichia coli, purified and used for the vaccination of common carp. Sequence analysis predicted that, Aha1 and OmpW are adhesins and antigenic. Common carp immunized with recombinant Aha1 and OmpW proteins showed significant antibody production and a relative percentage survival of 52 and 71 respectively indicating their protective efficacy against A. hydrophila infection.
African Journal of Microbiology Research, 2015
1 Animal Science UNIVASF, CCA, Petrolina, PE, Brazil. 2 Veterinary Medicine Course UNIVASF, CCA, Petrolina, PE, Brazil. 3 Tropical Animal Science of the UFRPE, Brazil. 4 Statistics Department, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. 5 Morphology Department, Universidade Federal de Santa Maria, Rio Grande do Sul, RS, Brazil. 6 Animal Science, UNIVASF, CCA, Petrolina, PE, Brazil.
Jurnal Akuakultur Indonesia
The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against Aeromonas hydrophila. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m3). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry. Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P<0.05) compared to the control. Relative percent survival of offspring at 5, 10,...
Protein Expression and Purification, 2007
The main aims of this study were to clone and express an outer membrane protein (OMP), OmpW, of Aeromonas hydrophila and to study its distribution in Aeromonas spp. Methods and Results: The gene encoding OmpW in A. hydrophila has been cloned and expressed in Escherichia coli. Primers were designed for amplification of full-length ompW gene and used for identification of this gene in different Aeromonas spp. Of the 42 Aeromonas strains tested, all the isolates were positive by polymerase chain reaction (PCR) except one strain of Aeromonas veronii biovar veronii (VTE338). None of the other gram-negative bacteria were positive by PCR with primers specific to ompW gene of A. hydrophila. Polyclonal antibodies were raised in rabbit against the purified recombinant protein and the reaction of these antibodies was confirmed by western blotting using the purified recombinant protein and 42 Aeromonas cultures grown at various salt concentrations. Conclusions: The ompW-based PCR method developed in this study was found to be 100% specific and 97% sensitive. Expression of OmpW protein of Aeromonas was found to be salt-dependant. Recombinant OmpW protein was found to be highly immunogenic in fish. Significance and Impact of the Study: To our knowledge, this is the first report on cloning and expression of OmpW protein of A. hydrophila. Full-length ompW gene amplification by PCR can be used for the detection of Aeromonas. Recombinant OmpW protein can be useful for vaccination of fish against Aeromonas spp.
Fish & Shellfish Immunology, 2006
Aeromonas sobria, along with A. hydrophila, has frequently been reported as a causative agent of motile aeromonas septicemia (MAS) in fish and other aquatic organisms. Currently, there are no precise tools to diagnose or vaccinate against this disease. The aim of the present study was to fractionate and characterize the outer membrane protein (OMP) antigen of A. sobria by serological and cellular techniques so as to identify immunoreactive molecules that might be useful in preparing immunodiagnostic and/or immunoprophylactic tools against A. sobria infection in goldfish. Eight fractions were isolated from the crude OMP antigen using sephacryl S-200 and DEAE-cellulose chromatography. The highest seroreactivity was observed in the gel-permeated protein G 1 which had an optical density (OD) of 0.72 nm, higher even than that of the crude OMP antigen, 0.63 nm. The serodiagnostic potential of G 1 was assessed by using dipstick ELISA. The in vitro goldfish lympho-proliferation ability of the fractionated antigen, G 1 A 3 , was higher than of all the other fractionated antigens and the crude OMP. Therefore, fractionated antigen G 1 (molecular wt 42-67 kDa) and G 1 A 3 (molecular wt 45-47 kDa) should be further studied in immunodiagnostic and/or immunoprophylactic preparations for A. sobria infection.
International Journal of Zoological Investigations, 2023
The experimental fish Channa striatus was divided as control and experimental groups. To find out the efficacy of Aeromonas hydrophila vaccine, the fish were infected with Gram negative Bacterial Strain Aeromonas hydrophila with a dosage of 0.1 ml. The samples of blood were collected on the 1 st , 3 rd , 5 th , 7 th and 14 th day intervals. The serum was evaluated for the biochemical parameters such as Protein, Albumin and Globulin, ratio of Albumin and Globulin and blood glucose level. SDS PAGE analysis was made to find out different protein fractions in the blood sample of fish which was treated earlier with Aeromonas hydrophila. Another set of fish was treated with heat killed and formalin (chemically) killed attenuated vaccine of A. hydrophila. Then the vaccinated fish was post challenged by infected with A.hydrophila to find out the efficacy of the vaccine. The results were tabulated and statistically analyzed. The present investigation revealed that the administration of vaccines could enhance the fish defense mechanisms. However, the heat killed (HK) vaccine showed great impact on improving the health condition of fish by enhancing its resistance capacity against pathogenic bacteria.