Solid substrate fermentation for cellulase production using palm kernel cake as a renewable lignocellulosic source in packed-bed bioreactor (original) (raw)

The study was to screen various microorganisms obtained from various laboratories situated in and around Chennai, Totally 73 species were grown in the culture medium. After the growth the mycelial dry weight, Protein content and cellulase activity were established. From this Alternaria, Bacillus, Aspergillus, Trichoderma, Fusarium and Penicillium shows good result for cellulose production. Among them, Trichoderma viride and Aspergillus terreus were recorded as cellulase producing species. Production of cellulase was analyzed by Dinitrosalicylic acid (DNS) and Carboxymethyl cellulase assay methods. Maximum enzyme production (1.76 U/ml) was achieved at 30°C, pH of 5.0 by Aspergillus terreus and on 6th day of incubation. When the culture medium was supplemented with Banana leaves, Rice husk, Millet husk, coir waste, wheat bran, saw dust, Rice straw and ground nut banana leaves (725 units) shows optimum production. Among various percentages 6% shows highest (825units) cellulose production.