Salvia candidissima Vahl.’ın uçucu yağ ve ekstraktının kimyasal bileşenlerinin, antioksidan ve antifungal aktivitesinin belirlenmesi (original) (raw)
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Objective. — To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L. Materials and method. — The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the discdiffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs. Results. — Major components were: 1,8-cineole (19.61%), sabinene (14.57%), a-pinene (9.76%), b-farnesene (6.04%), b-caryophyllene oxide (5.83%) and b-caryophyllene (5.02%). A low antioxidant activity was found (IC50 = 1.072 mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58 mm zone of inhibition (mean: 49 mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13 mg/ml V. agnus-castus seeds essential oils. Conclusion. — Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.
Determination of Antifungal and Antioxidant Activitiy of Salvia tomentosa
Journal of the Institute of Science and Technology, 2017
In this study, was determined antifungal and antioxidant activity of Salvia tomentosa. The essential oil of Salvia tomentosa was determined of the percentages of mycelium inhibition agains plant pathogenic fungi Rhizoctonia solani and Alternaria solani 40.63% and 60.13% respectively. To determine the antioxidant capacity, Free radical reduction (scavenging), iron reduction power (FRAP), copper reduction power (CUPRAC) and free radical cations reduction (TEAC), total phenolic (TP) and total flavonoids (TF) tests were performed. The results of the tests, scavenging activity DPPH lC 50 15.08±1.28 (µg mL-1), FRAP 1.81±0.27 mmol TE g-1 extract, CUPRAC 5.57±0.20 mmol TE g-1 extract and TEAC ise 3.30±0.33 lC 50 (µg mL-1) was determined. Total phenolic and total flavonoid was determined to be extracted content 131.34±8.39 mg GAE g-1 of extract and 35.05±2.82 QA g-1 respectively. Keyword; Antifungal, antioxidant, salvia tomentosa, total phenolic, essential oil ÖZET: Bu çalışma, Salvia tomentosa'nın antifungal ve antioksidant aktivitesi belirlenmesi amacıyla yürütülmüştür. Salvia tomentosa'nın uçucu yağı bitki patojeni fungus olan, Rhizoctonia solani ve Alternaria solani 'nin miselyum gelişim sırasıyla % 40.63 ve % 60.13 engellemiştir. Antioksidan kapasitesini belirlemek için, Serbest Radikal Giderme (DPPH), Demir İndirgeme Gücü (FRAP), Bakır İndirgeme Gücü (CUPRAC) ve Serbest Katyon Radikali Giderme (TEAC) antioksidan aktivite testleri ile Toplam Fenolik (TP) ve Toplam Flavonoid (TF) miktarı analizleri yapılmıştır. Yapılan antioksidan testleri sonucunda, DPPH lC 50 15.08±1.28 (µg mL-1), FRAP 1.81±0.27 mmol TE g-1 eksrakt, CUPRAC 5.57±0,20 mmol TE g-1 ekstrakt ve TEAC ise 3.30±0.33 lC 50 (µg mL-1) olarak belirlenmiştir. Toplam fenolik ve toplam flavanoid içeriği sırasıyla 131.34±8.39 mg GAE g-1 ekstrakt ve 35.05±2.82 mg QE g-1 ekstrakt olarak tespit edilmiştir.
Environmental and Microbial Biotechnology, 2020
Fungi are among the most devastating pathogens which their infections can cause food spoilage and mycotoxin production. These detrimental effects caused by fungi can negatively impact foodstuffs, economics and health. Another harmful factor is oxidation process, which causes food degradation. Antioxidants inhibit or delay the substrate oxidation. In order to avoid food spoilage, contamination, destruction, and oxidation and minimize the harmful effects of synthetic food preservatives, there is a growing interest to apply plant-based materials in food industry. It has been established that plants possess antifungal, antimycotoxigenic, and antioxidant activities as they contain various bioactive compounds. The essential oils (EOs) and plant extracts have been shown to act against fungal pathogens. Therefore, EOs and plant extracts are considered as natural, safe and effective agents to preserve foodstuffs and safeguard health.
Effects of Essential Oil in the Inhibition of Fungal Pathogen
Essential oils are highly concentrated substances extracted from flowers, leaves, stems, roots, seeds, barks, resins, or fruit rinds. These oils are often used for their flavour and their therapeutic or odoriferous properties, in a wide selection of products such as foods, medicines, and cosmetics. Extraction of essential oils is one of the most time-and effort-consuming processes. The way in which oils are extracted from plants is important because some processes use solvents that can destroy the therapeutic properties. There are wide number of ways to extract the Essential oil but the quality never remains the same. Here we are using the " Steam Distillation " method for extraction which is the cheapest way for the extraction of oils from the different parts of the plants. The present study constitutes an initiative to explore the anti-fungal activity of Trachyspermum ammi (ajwain oil), Cymbopogon winteranus (citronella Oil), Elettaria cardamomum (cardamom), Anethum gravelous (Dilseed oil) essential oil against fungal pathogen. Anti-fungal activity against Aspergillus niger were determined in terms of mycelial growth inhibition (MGI) and minimum inhibitory concentration (MIC). Trachyspermum ammi (ajwain oil) essential oil showed lower values of MIC against fungal pathogen. The essential oil also significantly inhibited the mycelial growth of the fungi. Hence, Trachyspermum ammi (ajwain oil) essential oil can be formulated into the antifungal ointments for topical applications in future.
2018
WOS: 000429081700043The essential oils from Salvia officinalis L., S. cryptantha (Montbret et Aucher ex Benth.) and S. tomentosa (Mill.) were extracted with hydrodistillation method and determined by using GC/MS analysis. This study investigated the in vitro effectiveness of essential oils and extracts of S. officinalis, S. cryptantha and S. tomentosa (collected from Tokat province) against eight fungal plant pathogens. Sterile PDA was prepared and then cooled to 40 degrees C, after which the plant essential oils and/or extract were added. PDA without extract was used as negative control, while PDA with a Propineb-containing fungicide was used as positive control. According to the GC/MS analysis, the principal components of S. cryptantha, S. tomentosa and S. officinalis essential oils were determined as eucalyptol (27.64%), Camphor (29.87%), a-pinene (11.91%); beta-thujene (40-69%), borneol (1.79-10.90%), camphor (0.40-7.25%); 3-thujonene (31.95%), camphor (28.53%), eucalyptol (7.35...
Archives of Biological Sciences, 2013
The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), ?-pinene (7.38%), myrcene (7.38%), ?-pinene (5.15%), ?-caryophyllene (4.13%) and ?-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.
Antioxidant and anti-candida activity of selected medicinal plants of Indian origin
Herba Polonica
Introduction Fungal disseases are the most common opportunistic infection. Objective The main aims of the study were to determine phenolic content and to evaluate the antioxidant and anti-candida activity of the selected Indian-origin plant extracts from the fruit pulp, stem, leaves, and seeds of three plants of Indian origin. Material and methods The extracts from Terminalia chebula, Thuja occidentalic and Syzygium jambolanum were investigated. The total phenolic content, antioxidant potential of different crude extracts was accessed using the free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Anti-candida activity was evaluated using disk diffusion method and broth dilution assay against Candida albicans. Results Ethanol and methanol extracts of Indian traditional plants possessed high radical scavenging activity: T. chebula 29.38±0.15, T. occidentalis 6.26±0.24 and S. jambolanum 25.64±0.18 at 0.32 mg/ml. The extra...
The Journal of Microbiology, Biotechnology and Food Sciences, 2023
This study aimed to evaluate the antifungal activity and the potential synergistic activity of ten plant extracts (EXs) and ten essential oils (EOs) obtained from the same plant species, namely Agrimonia eupatoria L., Arctium lappa L., Arnica montana L., Bidens cernua L., Corylus avellana L., Echinacea purpurea L. (Moench), Menta piperita L., Trifolium pratense L., Syzygium aromaticum L., and Angelica sinensis (Oliv.) Diels were used. Three species of the genus Candida, namely C. albicans, C. glabrata, and C. tropicalis, isolated from polluted estuarine water, were used in this study. The chemical composition of all EOs was evaluated by gas chromatography coupled with mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID) analysis. Firstly, the susceptibility of Candida spp. strains to EXs and EOs was screened by the agar disc diffusion method. Then the minimum inhibitory concentration (MIC) of the tested EXs and EOs was determined in a concentration range from 256 to 0.125 µL/mL. Following that, the effective EXs and EOs were divided into two groups (strong effect and weak effect) and tested in combination (strong + weak) at various concentrations depending on the tested strain to determine their potential synergistic effect using the chessboard pattern. The most sensitive strain to treatments with EXs and EOs tested alone and in mixtures was C. albicans. The most potent combinations with other EXs/EOs were observed with Syzygium aromaticum L., for both plant extract and essential oil, respectively. In this study the EOs and EXs tested in combination were much more effective than when acting on the growth of Candida spp. separately. So, the synergistic effect of the combinations of the EXs and EOs can lead to a reduction in the doses of commercial antifungals and can provide an effective way to reduce the infections caused by yeasts of the genus Candida.
Antioxidant and Antifungal Activity of Some Medicinal Plant Extracts
2017
In this study, the antioxidant and antifungal activities of extracts of Tabernaemontana alternifolia, Thuja occidantalis and Cajanus cajan were recognized. Total yields, radical scavenging activity and the antioxidant property of the extracts were investigated. The free radical scavenging activities of extract was documented as 41.3 ± 2.1 to 76.9 ± 6.1%, likewise. The antioxidant capacity of extracts was recognized between 153.4 ± 10.1 to 336.7 ± 24.2 mg/g extract. This extract was explored for antifungal activity by paper disc method against 2 fungi by using flucanazole as a standard drug. The 0.5 % level of Tabernaemontana alternifolia extract exhibited 100% inhibition till the 7th day of incubation when compare to control (Ascorbic acid). Statistical variances within fungi were significant at p<0.05. The extract was to some extent effective against all the fungi used in experiment.