Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells (original) (raw)
Related papers
Turkish Journal of Immunology , 2017
Objective: The purpose of this study was to explore the effect of propolis extract on CD8 + and CD4 + CD25 + regulatory T cell populations and granzyme expression in both cell populations, as propolis has been suggested as an immunomodulatory agent. Materials and Methods: PBMC containing 1x10 6 cells/ml from cervical cancer stage 3 patients were isolated and cultured with and without propolis extract. The cells were plated in a 24-well plate with RPMI medium supplemented with 10% FBS, 10 U/ml penicillin, 100 U/ml streptomycin, 2 mM L-glutamine and 50 μM 2-ME. Propolis extract was added (5, 10, 25 and 50 μg/ml) to PBMC culture. Negative control was made from PBMC culture without propolis extract for comparison. The cells were incubated for 48 hours in a 5% CO 2 incubator at 37ºC. The proportion of CD8 + and CD4 + CD25 + regulatory T cells that expressed granzyme were measured by flow cytometry. Results: The results obtained that the level of CD4 + , CD4 + CD25 + , and CD8 + T cells were not markedly depleted in propolis treatment in all doses compared to control group. The percentage of CD8 + Granzyme + and CD4 + CD25 + Granzyme + were significantly different in groups of propolis treatment compared to control group. Conclusion: In summary, propolis extract may serve as an immunomodulatory agent in cervical cancer patients. Propolis extract could modulate the expression CD4 + CD25 + T cells expressing granzyme and CD8 + T cells expressing granzyme while it has no effect on CD4 + , CD4 + CD25 + , and CD8 + T cells. Öz Amaç: Çalışmanın amacı, propolis ekstresinin bağışıklık düzenleyici bir madde olarak CD8 + ve CD4 + CD25 + düzenleyici T hücrelerindeki granzim ifadesi üzerine olan etkisini incelemektir. Gereçler ve Yöntemler: Evre 3 serviks kanserli hastalardan elde edilen 10(6) hücre/mL konsantrasyonundaki Periferik Mononükleer Kan Hücreleri(PMKH) izole edilerek propolis eklenen ve eklenmeyen kaplarda kültüre edildi. Hücreler, %10 FBS, 10 U/ml penisilin, 100 U/mL streptomisin, 2mM L-glutamin ve 50 mikroM 2-ME içeren 24 kuyulu kültür kaplarına paylaştırıldı. Bir PMKH popülasyonu Propolis uygulanmadan kontrol grubu olarak oluşturuldu. Hücreler %5 CO2 içeren kuluçka makinesinde(inkübatör) 48 saat kültüre edildi. Granzim ifade eden CD8+, CD4+CD25+ düzenleyici T lenfositleri akan hücre ölçer ile irdelendi. Bulgular: Propolis uygulanan CD4 + , CD4 + CD25 + ve CD8 + T lenfositlerinin sayıları anlamlı ölçüde azalmadı. CD8 + Granzim + ve CD4 + CD25 + Granzim + hücrelerin sayıları, propolis uygulanan hastalarda farklı idi. Sonuç: Sonuç olarak, propolis ekstresi serviks kanserli hastalarda bağışıklığı değiştiren bir madde olarak etki etmektedir. Ekstre, CD4 + CD25 + T lenfositleri ile CD8 + T lenfositlerinde granzim ifadesini değiştirmekte, ancak ekstrenin CD4 + , CD + CD25 + ve CD8 + T lenfositlerinde granzim ifadesine etkisi görülmemektedir.
Mellifera, 2002
The antimetastatic efficacy of a water-soluble derivative of propolis (WSDP) was studid. Tumor was a transplantable mammary carcinoma (MCA) of CBA mouse. Metastasis in the lung were generated by 2x105 viable tumor cells intra venous (iv). WSDP was given intra peritoneal (ip) at doses of 50 or 150 mg/kg before or after tumor cell inoculation. Therapies reduced the number of metastases in the lung. It is likely that antimetastatic activity of the WSDP is mainly mediated by immunomodulatory activity. Changes in several immunological parameters such as production of activacting factor (LAF) by peritoneal macrophages and the efficacy of those macrophages to kill tumor cell in vitro, responses of lymphocytes to mitogen, and weight and cellularity of spleen, respectively, correlated well with antimetastatic properties of the WSDP. Based on results we postulate that the antimetastatic activity of propolis includes a pronounced immunomodulatory activity mainly toward augmentation of nonspecific antitumor resistence in mice via macrophage activation.
Immunomodulation and Antigenotoxic Effects of Propolis in Paclitaxel-Treated Rats
Egyptian Journal of Zoology
The current study aimed to investigate the potential immunomodulatory and antigenotoxic impacts of propolis in paclitaxel (PTX)-treated rats. Twenty four male Sprague Dawley rats were used in the present study and randomly/equally divided into four groups; control group, PTX group that was intraperitoneally injected with 5 mg/kg PTX (once/week), propolis group that received orally/daily 50 mg/kg propolis, and the last group received both PTX and propolis. All treatments were given for four weeks. The results showed a significant upregulation in the nuclear factor kappa B (Nf-b) gene expression in the spleen of PTX group, as compared with the control group. In addition, substantial increases in tumor necrosis factor-, interferon-, C-X-C motif ligand 10, CC motif ligand 2, and interleukin (IL)-4 concentrations, while a significant reduction in IL-10 concentration, were detected in the spleen of PTX group compared with the control group. Moreover, PTX resulted in a significant elevation in the frequency of micronucleated polychromatic erythrocytes in bone marrow cells compared with the control group. On the other hand, the treatment with propolis alone did not significantly affect all of these parameters, as compared with the control group. However, propolis decreased significantly all recorded side effects in the PTX-treated rats. In conclusion, propolis can be used as an adjunct with PTX to modulate the cytokines and chemokines release of splenic immune cells, as well as to counteract the genotoxic effect of PTX on bone marrow cells, through downregulating the splenic Nf-b gene expression, and reducing the bone marrow micronuclei formation, respectively.
Use of Propolis in Cancer Research
Interest to develop new anticancer drugs and to design combination treatments with little or no secondary effects provides new scope for traditional phytochemicals in chemoprevention and therapy. Propolis is a known source of polyphenols, and flavonoids found in them have been widely studied as biochemical markers for botanical origin and in explaining their antioxidant capacity as a key factor in chemoprevention. Antimicrobial, anti-inflammatory and anticancer biological activities of propolis are known. Studies of cancer cells to measure the anticancer effect of propolis are designed with one carefully chosen component, and with extracts applied to cells in culture media. The antitumor effect of propolis and caffeic acid phenethyl ester (CAPE), bioactive compound of propolis extract, is seen to be associated with its ability to initiate apoptosis of cancer cells. Chrysin is a flavonoid of interest to identify signaling molecules related to cancer. As cancer cells develop multidrug resistance (MDR) during chemotherapy, this opens a new avenue of research on cellular mechanisms of propolis components in combined treatments designed to overcome MDR.
Immunomodulation by water-soluble derivative of propolis: a factor of antitumor reactivity
Journal of Ethnopharmacology, 2003
The antimetastatic efficacy of a water-soluble derivative of propolis (WSDP) was studied. Tumor was a transplantable mammary carcinoma of CBA mouse. Metastases in the lung were generated by 2 )/10 5 viable tumor cells i.v. WSDP was given intraperitoneally at doses of 50 or 150 mg/kg before or after tumor cell inoculation. Therapies reduced the number of metastases in the lung and tumor growth was suppressed significantly by WSDP. It is likely that antimetastatic activity of the WSDP is mainly mediated by immunomodulatory activity. Changes in several immunological parameters such as production of lymphocyte activating factor by peritoneal macrophages and the efficacy of those macrophages to kill tumor cell in vitro, responses of lymphocytes to mitogen, and weight and cellularity of spleen, respectively, correlated well with antimetastatic properties of the WSDP. Based on results we postulate that the antimetastatic activity of propolis includes a pronounced immunomodulatory activity mainly toward augmentation of nonspecific antitumor resistance in mice via macrophage activation. #
Antitumor Activity of Propolis on Differantiated Cancer Cell Lines
Medicine Science | International Medical Journal, 2012
Propolis is a natural bee product with several pharmacological activities. Nowadays, it is also investigated for its antitumor properties. There are controversies on the antitumor activity of propolis, not all tumor cells seem to respond to propolis treatment. The aim of our study is to evaluate the activity of propolis on differentiated thyroid cancer cell lines. Tyripan blue test and MTT assay were performed to evaluate the cell viability of B-CPAP cells after propolis treatment and compared with propolis free cells and normal thyroid cells. Evaluation with light microscopy revealed after treatment with propolis, cell count decreased and B-CPAP cells displayed morphologic changes with a smaller appearance. Tyripan blue exclusion test showed a decrease in cell viability in different concentrations of propolis at 48 and 72 h. Cell viability was measured by MTT after the treatment of B-CPAP cells with propolis at 72 h. The decrease in cell viability was statistically significant only at 0.0001 diluted propolis (p=0.048). The decrease in cell viability in normal thyroid cell line was not statistically significant. In conclusion, propolis has weak antitumor activity on differentiated thyroid cell line which may contribute to different degrees of sensitivity of propolis among cancer cells.
Biological Effects of Propolis on Cancer
Turkish Journal of Agriculture - Food Science and Technology, 2020
Propolis is a special resin and wax material collected from the leaves and shells of trees, buds and shoots of plants by honey bees (Apis mellifera L.). In recent years, many researchers have studied the chemical composition, biological activity and pharmacological properties of propolis. The colour, odour and pharmacological properties of the propolis composition also vary as the composition changes depending on the plant, region, season and bee colony. Flavonoids, aromatic acids, phenolic acids and esters are the main compounds responsible for the biological activity of propolis. A number of studies have been conducted on the use of propolis or its active ingredients in the treatment of cancer. It has been observed that the use of propolis did not cause side effects according to in-vitro and in-vivo studies. Propolis should be extracted with different compounds for use in biological assays. The most commonly used compounds for extraction are ethanol, methanol, oil and water. A num...
Apoptosis of human Breast Cancer Cells induced by Ethylacetate Extracts of Propolis
American Journal of Biochemistry and Biotechnology, 2010
Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.