Electrochemical and spectral study of the interaction of antitumoral drug mitoxantrone with DNA (original) (raw)

Revue Roumaine De Chimie, 2005

Abstract

The interaction between the antitumor drug mitoxantrone and double stranded DNA in phosphate buffer solution was investigated by cyclic and linear voltammetry as well as by UV-VIS absorption spectroscopy. The results suggest that the diffusion coefficients of mitoxantrone determined by cyclic and linear voltammetry with stationary and rotating disc electrode decreased in the presence of DNA. The binding constants and binding site size of mitoxantrone to DNA were obtained by a titration curve and linear and nonlinear regression analysis of the data. The results indicate that mitoxantrone binds to calf thymus DNA with a high affinity and the electrochemical methods provide a useful complement to spectral methods in the characterization of the drug-DNA binding equilibria.

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