Snails And Fish As Pollution Biomarkers In Lake Manzala And Laboratory C: Laboratory Exposed Snails To Chemical Mixtures (original) (raw)
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Egyptian Journal of Aquatic Biology and Fisheries
The quality of water in Lake Burullus was investigated to evaluate its effect on certain biochemical and histological aspects on Biomphalaria alexandrina snail and Oreochromis niloticus fish. The examined lake water quality criteria showed high levels of total dissolved solids (TDS), conductivity (EC), and levels of Pb and Fe. Sites that adjacent to point of discharging of waste waters showed higher levels of heavy metals and the least levels in dissolved oxygen (DO). The results showed that most of the examined biochemical parameters indicated affected liver (high levels in AST, ALP & TP and alteration in glucose & urea levels). Samples of Bahr Tera site in El-Hamoul center and Shakhloba site in Sidi-Salem were the most affected, while samples of Prempal site in Mottobus center were the least. Snails' histopathological examination showed degeneration, edema, hyper trophy and vaculation in head-foot muscles, degeneration and necrotic changes in the digestive gland, irregular shape in mature ova and reduction in sperm number in hermaphrodite gland. As well, fish histopathological investigation illustrated by necrotic change in most examined organs, the common features were: fiber splitting in muscles, cognation of blood vessels of gill filaments, vacuolar degeneration in liver and atrophy in glomeruli and epithelial cells of renal tubules in kidney. It was concluded that Lake Burullus water quality in wastewater discharge areas, adversely affects B. alexandrina snails and O. niloticus fish, and both can be used as bio-indicators for the presence of pollutants in their habitats.
Snails and Fish as Pollution Biomarkers in Lake Manzala and Laboratory A: Lake Manzala Snails
Fisheries and Aquaculture Journal, 2015
Physiological, hematological and biochemical parameters have been used as biomarkers for water quality in snail samples collected from Lake Manzala. The results showed significant increase in AST, ALT, and ALP in planorbis and physa snail samples collected from Dakahlya site in Lake Manzala. Most of snails are collected from of Port-Said and Dakahlya sites showed significant increase in urea. On the other hand, alteration in creatinine values in samples from different lake sites was recorded. Significant increase of total protein level and total bilirubin was obtained in all samples. Most of snail samples showed significant decrease in hemocytes count. The oxidative enzymes (CAT, GGT and GST) recorded alteration in their activity. Regarding Histopathological observations, in the foot region of Biomphalaria snails collected from Port Said and Dakahlya governorates are the most affected. The head foot showed splitting in the longitudinal and oblique muscle fibers and increased empty spaces within muscle. Shrinkage, focal areas of necrosis, large fat vacuoles and enlargement were observed in the salivary gland. Snail's ganglia showed enlargement of neurosecretory neurons, degeneration with large vacuoles and fibrosis. Hepatopancreas became much more distorted with necrosis, atrophy, degeneration and fat vaculation especially in Port Said and Damietta samples. Also, hepatopancreatic acini filled with different developmental stages of S. mansonai cercariae were observed in Biomphalaria snails collected from Port Said. Severe degenerative changes were observed in most of gonad's cells including ova and sperms especially in snails collected from Damietta. Also, Biomphalaria snails collected from Lake Mazala showed accumulation of heavy metals in the head foot tissues. In conclusion, the severe alteration and degeneration recorded in the physiological and hematological parameters and also histopathological observations are clear evidence for the pollution of the water from which these snails were collected.
Kurdistan University of Medical Sciences, 2019
Urban wastewater contains various detergents, pesticides, pharmaceutical medications, cosmetics, hygiene products, and heavy metals. Discharge of municipal sewage into surface water affects the health of aquatic organisms through altering biochemical markers and accumulating heavy metals in various tissues. The present study aimed to evaluate the toxic effects of municipal sewage on the biochemical markers and bioaccumulation of cadmium and lead in freshwater snail (Galba truncatula) during 14 days. Concentrations of toxic metals in the snails were determined using ICP-OES-PerkinElmer, and biochemical parameters were measured via UV-Vis spectroscopy. The results indicated significantly lower levels of glycogen and total antioxidant in the cells, as well as the significantly lower activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acetylcholine esterase, catalase, and glucose-6-phosphate dehydrogenase in the Galba truncatula exposed to sewage compared to the control. However, the activities of lactate dehydrogenase ,glutathione peroxidase, and malondialdehyde were significantly higher in the snails exposed to sewage compared to the control. Moreover, bioaccumulation of cadmium and lead was observed to increase in the experimental groups exposed to sewage. Alterations in biochemical parameters in the G. truncatula exposed to sewage could be due to the toxic effects of various environmental pollutants in municipal wastewater. According to the findings, oxidative damage to the vital tissues of G. truncatula was associated with the bioaccumulation of cadmium and lead, depletion of total antioxidant levels, changes in biochemical parameters, and lipid peroxidation in soft tissues.
Bioaccumulation of heavy metals in freshwater snails in relation to lining of water courses in Egypt
Journal of Bioscience and Applied Research, 2016
The present study was aimed to determine the heavy metal concentrations in whole body of fresh water snails in relation to lining of water courses in two governorates (Behaira (Nubaria) and Giza) Through four successive seasons from Septemper 2013, to October 2014. The obtained results indicated that the electrical conductivity (EC) and iron metal (Fe) showed the highest values and generally the physicochemical measurements indicated that no significant difference between the two tested habitats, however, the conductivity of water in lined water bodies was lower than those of unlined ones. The present study recorded 14 species of snails belonging to class Gastropoda; these species belonged to Pulmonate and Prosobranch snails. The density of all recorded Pulmonate snails in lined sites were lower than those in unlined ones. On the other hand, the abundance of Prosobranch snails was higher in numbers in the lined sites than those in the unlined. In Nubaria the most abundant snails were Melanoides tuberculata and Theodoxus niloticus and exhibited their maximum percentages in the lined (82.15%) and unlined (72.34%) sites during summer, respectively. While, in Giza Governorate Physa acuta snails had the highest percentages among collected snails approximately at all seasons, and exhibited its maximum percentages during spring in lined (90.22%) and unlined sites (85.38%). Correlations between physicochemical parameters and the collected snails, revealed that there were different negative or positive correlations depending on the sites of investigation. The bioconcentration factor of heavy metals in snail's soft tissues at the investigated in lined and unlined sites compared to the content of the metals (Cd, Cu, Fe, Pb and Zinc) in the water showed high variation in BCF values depending on the type of metal and the snail. Generally P. acuta (7626.95μg.g-1 DW for Fe) and H. duryi (8787.10 μg.g-1 DW for Cu) were the highest snails of accumulated metal contents in the tested sites of the two surveyed governorates, respectively.
Snails And Fish As Pollution Biomarkers In Lake Manzala And Laboratory B: Lake Manzala Fish
2016
This work aimed to examine <em>Oreochromis niloticus</em> fish from Lake Manzala in Port Said, Dakahlya and Damietta governorates, Egypt, as a bio-indicator for the lake water pollution through recording alterations in their hematological, physiological, and histopathological parameters. All fish samples showed a significant increase in levels of alkaline phosphatase (ALP), creatinine and glutathione-S-transferase (GST); only Dakahlya samples showed a significant increase (p<0.01) in aspartate aminotransferase (AST) level and most Dakahlya and Damietta samples showed reversed albumin and globulin ratio and a significant increase in γ-glutamyltransferase (GGT) level. Port-Said and Damietta samples showed a significant decrease of hemoglobin (Hb) while Dakahlya samples showed a significant decrease in white blood cell (WBC) count. Histopathological investigation for different fish organs showed that Port-Said and Dakahlya samples were more altered than Damietta. The mus...
Acute toxicity of some heavy metals to the fresh water snail, Theodoxus niloticus (Reeve, 1856)
The Egyptian Journal of Aquatic Research, 2018
Three toxicity experiments were carried out separately to study the toxicity effects of Zn, Fe and Pb on adult freshwater snail Theodoxus niloticus (Gastropod, Prosobranchia, Nertidae). The LC50 values for the 96 hr exposures of Zn, Fe and Pb on Theodoxus niloticus were 12.199, 8.6 and 18 mg/l respectively. These values increased by decreasing the time of exposure. Fe was more toxic followed by Zn and Pb to Theodoxus niloticus. The rate of mortality increased by increasing the exposure period and concentration of metals in solution. Moreover, bioconcentration of Zn, Fe and Pb in soft parts and shell increased gradually by increasing the concentrations of each metal in solutions. After 96 hr of exposure, the bioaccumulation factors for each metal were decreased from highest values in the control to the lowest one with the highest concentration of the metal. It is difficult to compare LC50 values for metals for this species with those for other gastropod species due to different abilities of closely related taxa or the species belonging to the same genus, which live in the same habitats, to accumulate metals.
Applied Sciences
The present investigation focused on the toxicity test of cadmium (Cd), copper (Cu), nickel (Ni), lead (Pb) and zinc (Zn), utilizing two groups of juvenile and adult apple snail Pomacea insularum (Gastropod, Thiaridae) with mortality as the endpoint. For the adult snails, the median lethal concentrations (LC50) values based on 48 and 72 h decreased in the following order: Cu < Ni < Pb < Cd < Zn. For the juvenile snails, the LC50 values based on 48 and 72 h decreased in the following order: Cu < Cd < Ni < Pb < Zn. The mussel was more susceptible to Cu than the other four metal exposures, although the juveniles were more sensitive than the adults because the former had lower LC50 values than the latter. This study provided essential baseline information for the five metal toxicities using P. insularum as a test organism, allowing comparisons of the acute sensitivity in this species to the five metals. In conclusion, the present study demonstrated that P. insula...
Chemosphere, 2010
This study investigates the biomarkers of oxidative stress and heavy metal accumulation for assessing ecotoxicological effects of urban metal pollution in Alexandria city, Egypt. This investigation was performed in the digestive gland of roadside Theba pisana snails obtained from six different sites in the city. Relationships between heavy metal (Zn, Cu, Pb and Cd) concentrations and oxidative stress indicators were also examined. The results showed that mean concentrations of the measured elements (lg g À1 dry weight) were higher in polluted sites when compared to the background levels of the reference site. The pattern of metals accumulation at all sites was Zn > Cu > Pb > Cd. In the metal polluted sites, snails displayed higher mean of catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities, lower reduced glutathione (GSH) content and higher levels of lipid peroxidation (LPO) compared to snails from the reference site. All oxidative stress parameters were positively correlated to heavy metal concentrations except GSH content which exhibits negative correlation with the concentrations of these metals. Our results suggest that the tested antioxidant defenses are sensitive parameters that could be useful as oxidative stress biomarkers in snails exposed to the actual metals in the environment. These biomarkers when complemented with metal accumulation in the digestive gland of snails may provide a powerful biomonitor for the assessment of environmental metal pollution.
Metal pollution results in many dangerous consequences to the environment and human health due to the bioaccumulation in their tissues. The present study aims to measure the bioaccumulation factor of the Manganese (Mn) heavy metal in Biomphlaria alexandrina snails' tissues and water samples. The present results showed the concentration of Mn heavy metal in water (87.5 mg/l) and its bioaccumulation factor in Helisoma duryi tissue was higher than that in tissues of Physa acuta and B. alexandrina snails. Results showed that 87.5 mg/l Mn concentration had miracidial and cercaricidal activities. Also, this concentration decreased the mean total number of the hemocytes after exposure for 24h or 48h, while increased both the mean mortality and phagocytic indices of the hemocytes of exposed snails. It caused alterations in the cytomorphology of the hemocytes of exposed snails after 24 or 48h, where, the granulocytes had irregular cell membrane, and forming pseudopodia. Besides, both lev...
Toxicology and Industrial Health, 2020
Some snails ( Achatina spp) can be used as a biosensor of heavy metal poisoning. This study thus estimated some heavy metal levels, antioxidant markers, and lipid profiles of snails handpicked around cement factory vicinities in Ogun State, Nigeria. Snails and soil samples were collected from Oke, Ewekoro, Papalanto, and Mowodani Imeko-Afon (control site). Lead (Pb), cadmium (Cd), and arsenic (As) levels were estimated in the soil, snail foot, hemolymph, and shell using Atomic Absorption Spectrophotometry. Triacylglycerol (TAG), phospholipids (PHOL), cholesterol (CHOL), malondialdehyde (MDA), and reduced glutathione (GSH) levels, as well as glutathione- S-transferase (GST), lactate dehydrogenase (LDH), and arylesterase (AR) activities in the hemolymph, were estimated spectrophotometrically. The snails collected from the Oke site had the highest foot Pb (274.66 ± 13.50 mg/g tissue), CHOL, TAG, PHOL levels, and GST activity when compared with other sites. Snails collected from Papa ha...