Screening of the effects of Zinc oxide based nanofertilizers on the germination of Lathyrus sativa L. seeds (original) (raw)
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International Journal of Molecular Sciences
The growing interest in the use of zinc oxide nanoparticles (ZnO NPs) in agriculture creates a risk of soil contamination with ZnO NPs, which can lead to phytotoxic effects on germinating seeds and seedlings. In the present study, the susceptibility of germinating seeds/seedlings of pea and wheat to ZnO NPs of various sizes (≤50 and ≤100 nm) applied at concentrations in the range of 100–1000 mg/L was compared. Changes in metabolic profiles in seedlings were analyzed by GC and GC-MS methods. The size-dependent harmful effect of ZnO NPs on the seedling’s growth was revealed. The more toxic ZnO NPs (50 nm) at the lowest concentration (100 mg/L) caused a 2-fold decrease in the length of the wheat roots. In peas, the root elongation was slowed down by 20–30% only at 1000 mg/L ZnO NPs. The metabolic response to ZnO NPs, common for all tested cultivars of pea and wheat, was a significant increase in sucrose (in roots and shoots) and GABA (in roots). In pea seedlings, an increased content o...
Herein, we investigated potential phytotoxicity of zinc oxide nanoparticles (ZnONPs) on seed yield, focusing on particle size-, morphology-, and concentration-dependent responses of multiple antioxidant defense biomarkers, in soil-grown soybean (Glycine max cv. Kowsar) during its lifecycle. To this end, we synthesized three types of morphologically unique ZnONPs (spherical/ 38nm, floral-like/ 59nm, and rod-like/ >500nm); all with high purity, triclinic crystal structure and negative surface charge; and compared the toxicity with Zn2+ ions. Each pot received two seeds, placed in soil inoculated with N-fixing bacteria (Rhizobium japonicum) and grown outdoor for 120 days. Our findings demonstrated a significant particle size-, morphology-, and concentration-dependent influence of ZnONPs on seed yield, lipid peroxidation, and various antioxidant biomarkers in soybean. Our spherical 38nm ZnONPs were the most protective compared to the floral-like 59nm ZnONPs, rod-like >500nm ZnONPs...
CABI agriculture and bioscience, 2024
To ascertain the ideal dosage of ZnO NPs (Zinc Oxide Nanoparticles), we conducted an investigation on the priming effects of varying concentrations of ZnO NPs on germination and physio-biochemical parameters of wheat. In this study, ZnO NPs were synthesized and characterized for their physico-chemical properties followed by confirmation of the formation of ZnO NPs. Throughout this study, wheat seeds were subjected to ZnO NPs at various concentrations of 5, 50, 100, 250, and 500 ppm for a period of 4 h via continuous aeration. The primed seeds were sowed in plastic bags, allowed to grow for 21 days, following which comprehensive evaluations of physio-biochemical attributes were conducted. At 250 ppm, an impressive 100% of seeds successfully germinated compared to the control group. The examined physiological factors such as shoot length, root length, and fresh as well as dry weights of leaf and root tissues all exhibited notable increases with the ascending concentrations of ZnO NP up to 250 ppm. However, beyond this threshold, at 500 ppm, these parameters experienced a decline. Inductively coupled plasma atomic absorption spectrophotometer (ICP-AAS) measurements validated the progressive increase in Zinc content in the nanoprimed seedlings, further affirming the dose-dependent trend. Zinc oxide nanoparticles notably improved key biochemical features, including elevated levels of total chlorophyll, malondialdehyde (MDA), total protein, and the accumulation of osmolytes such as proline and glycine-betaine. Additionally, the presence of ZnO NPs led to increased activity of antioxidant enzymes like superoxide dismutase (SOD) and catalase in a dose-dependent mananer. Collectively, the amassed data underscores the efficacy of the 250 ppm ZnO NPs treatment, which emerged as superior in comparison to both the control group and other administered treatments. These findings underscore the potential of ZnO NPs at a concentration of 250 ppm as a valuable seed nanopriming agent, effectively enhanced germination and robust early-stage growth in young plants.
Zinc Oxide Nanoparticles Help to Enhance Plant Growth and Alleviate Abiotic Stress: A Review
Current Protein & Peptide Science, 2021
Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is the main concern of environmentalists at present. To achieve food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps in better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) influence the negative effects of abiotic stresses. The application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-...
Biomolecules, 2020
Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem-derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol-β-coniferyl alcohol ether glucoside in the plantlets grown on the...
2021
In order to assess the effects of zinc oxide nanoparticles (ZO-NP) on soybean seed germination under drought stress induced by polyethylene glycol, a factorial experiment was conducted in a completely randomized design at the University of Mohaghegh Ardabili, Ardabil, Iran, in 2013. The experiment consisted of three levels of drought (0, -0.5 and -1 MPa) and various concentrations of ZO-NP (0, 0.5 and 1 g lit-1). The soybean seeds used in this experiment was the DPX prepared from the Moghan Agricultural Research Center. The results showed that drought stress reduced the activity of peroxidase, superoxide dismutase, malate synthase and isocitrate lyase enzymes together with the germination rate, ascorbate and alpha-tocopherol concentration. However, the use of ZO-NP could counteract the adverse effects of drought, so that non-enzymatic antioxidant levels and antioxidant enzyme activity was increased and this improved the germination rate. Applying ZO-NP increased the activity of isoc...
A field experiment was conducted to evaluate the effects of biologically-synthesized zinc oxide nanoparticles (ZnONPs), zinc sulfate (ZnSO 4) and their interactions on carbohydrates, proteins, endogenous acidic phytohormones and minerals contents, as well as the activities of some hydrolytic enzymes of common bean (Phaseolus vulgaris L.) plants. The results observed that carbohydrate and protein contents in common bean plants were enhanced when the plants treated with ZnONPs (25, 50, 100 and 200 ppm), ZnSO 4 (50 and 100 ppm) and their interactions. The tested treatments showed different responses in amylase and protease activities in addition to the stimulation which found in phytohormones (Gibberellic acid, Indole acetic acid and Abscisic acid). These treatments markedly increased nitrogen (N), phosphorus (P) and potassium (K) contents and these increases reached to 45.7 %, 55.6 % and 23.8 %, respectively. Our results obtained that biologically-synthesized zinc oxide nanoparticles have positive effects on carbohydrate, protein, amylase, protease, gibberellic acid (GA 3), indole acetic acid (IAA), abscisic acid (ABA), nitrogen, phosphorus and potassium contents of common bean plants.