Simultaneous determination of lidocaine hydrochloride, hydrocortisone and nystatin in a pharmaceutical preparation by RP-LC (original) (raw)
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TheScientificWorldJournal, 2014
A new specific, precise, accurate, and robust TLC-densitometry has been developed for the simultaneous determination of hydrocortisone acetate and lidocaine hydrochloride in combined pharmaceutical formulation. The chromatographic analysis was carried out using a mobile phase consisting of chloroform+acetone+ammonia (25%) in volume composition 8:2:0.1 and silica gel 60F254 plates. Densitometric detection was performed in UV at wavelengths 200 nm and 250 nm, respectively, for lidocaine hydrochloride and hydrocortisone acetate. The validation of the proposed method was performed in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and robustness. The applied TLC procedure is linear in hydrocortisone acetate concentration range of 3.75÷12.50 μg·spot(-1), and from 1.00÷2.50 μg·spot(-1) for lidocaine hydrochloride. The developed method was found to be accurate (the value of the coefficient of variation CV [%] is less than 3%)...
Journal of the Iranian Chemical Research, 2010
The multivariate calibration method, partial least square regression (PLS) was applied for the simultaneous spectrophotometric determination of Lidocaine (LID) and Hydrocortisone acetate (HCA) in their mixtures. The parameters of chemometric technique were optimized and the proposed method was validated with synthetic samples and applied to analyze these drugs in pharmaceutical products with good accuracy and precision. The results were compared with the HPLC method. The squares of correlation coefficients (R 2) for predicted LID and HCA with the proposed method in test samples were 0.9970 and 0.9964, respectively. The relative standard deviations for commercial products were less than 1%.
Journal of Chromatography A, 2005
In this paper, there was developed a sensitive, precise and accurate reversed-phase liquid chromatographic (RP-HPLC) method and validated for simultaneous determination of lidocaine hydrochloride, dexamethasone acetate (DA) and calcium dobesilate (CD) in suppositories and ointment. Also there was achieved a parallel analysis of buthylhydroxyanisol, as a preservative, and hydroquinone, as a degradation product of calcium dobesilate, present in these dosage forms. The relative standard deviation (RSD) values for all five compounds indicated a good precision and accuracy of the RP-HPLC method. Method is selective, sensitive and reproducible with good recovery values and can be applied in simultaneous determination of all mentioned compounds.
https://www.ijrrjournal.com/IJRR\_Vol.7\_Issue.1\_Jan2020/Abstract\_IJRR0011.html, 2020
Objective: To develop extraction procedures for extracting Lidocaine from various pharmaceutical dosage forms (ointment, gel, injection, aerosol, transdermal patch) and to analyze them by development of accurate, precise and robust reverse phase high performance liquid chromatography method. Method: Chromatographic procedures were developed using Chromatopak, Peerless C18 column (Column dimensions: 250 mm x 4.6 mm, 5 μm) with mobile phase comprising of Dipotassium monohydrogen phosphate buffer (10Mm): ACN in ratio 20:80 at a flow rate of 1ml/min, with detection wavelength at 263nm. The retention time of Lidocaine was found to be at 5.43±0.03. Results: The method was validated according to ICH guidelines (Q2) R1.Linearity of LID was found in concentration range of 20-100ug/ml with r2=0.999. Limit of Detection and Limit of Quantification were found to be 1.54ug/ml and 4.68ug/ml. %RSD values for intraday and interday precision were also found to be >2%. Accuracy studies were also in range between 95%-105%. The method proved to be robust when chromatographic parameters like Ph, mobile phase ratio, flow rate, wavelength were altered. Conclusion: The % Assay values of marketed formulation were found to be within prescribed range. Thus this proposed RP-HPLC method can be used in routine quality control analysis of LID from its various pharmaceutical dosage forms.
Journal of Chromatographic Science, 2015
This work was concerned with development, optimization, application and validation of reversed phase high performance liquid chromatography (RP-HPLC) and thin layer chromatography (TLC)-densitometric methods for analysis of cetylpyridinium chloride, chlorocresol and lidocaine in Canyon ® gel. The first developed RP-HPLC method depended on chromatographic separation on a ZORBAX Eclipse Plus C 8 column, with elution with a mobile phase consisting of 0.05% phosphoric acid solution : acetonitrile : methanol (15 : 24 : 61, by volume), pumping the mobile phase at a flow rate of 1.00 mL min −1 , with ultraviolet detection at 220 nm. While in the subsequently developed method, the TLC-densitometric method, complete separation of the studied mixture was achieved using methanol : acetone : acetic acid (7 : 3 : 0.2, by volume) as a mobile phase, aluminum plates precoated with silica gel 60 F 254 as a stationary phase and 215 nm as the scanning wavelength. Factors affecting the developed methods were studied and optimized; moreover, methods had been validated as per the International Conference of Harmonization guideline and the results indicated that the suggested methods were reproducible, reliable and applicable for rapid routine analysis. Statistical comparison of the two developed methods with the reported HPLC ones using Fand Student's t tests showed no significant difference.
Chromatographia, 2013
A new liquid chromatographic (LC) method for simultaneous determination of lidocaine hydrochloride (LH) and tribenoside (TR) along with their related compounds in pharmaceutical preparations is described. Satisfactory LC separation of all analytes after the liquid-liquid extraction (LLE) procedure with ethanol was performed on a C 18 column using a gradient elution of a mixture of acetonitrile and 0.1 % orthophosphoric acid as the mobile phase. The procedure was validated according to the ICH guidelines. The limits of detection (LOD) and quantification (LOQ) were 4.36 and 13.21 lg mL -1 for LH, 7.60 and 23.04 lg mL -1 for TR, and below 0.11 and 0.33 lg mL -1 for their impurities, respectively. Intra-and inter-day precision was below 1.97 %, whereas accuracy for all analytes ranged from 98.17 to 101.94 %. The proposed method was sensitive, robust, and specific allowing reliable simultaneous quantification of all mentioned compounds. Moreover, a comparative study of the RP-LC column classification based on the quantitative structure-retention relationships (QSRR) and column selectivity obtained in real pharmaceutical analysis was innovatively applied using factor analysis (FA). In the column performance test, the analysis of LH and TR in the presence of their impurities was carried out according to the developed method with the use of 12 RP-LC stationary phases previously tested under the QSRR conditions. The obtained results confirmed that the classes of the stationary phases selected in accordance with the QSRR models provided comparable separation for LH, TR, and their impurities. Hence, it was concluded that the proposed QSRR approach could be considered a supportive tool in the selection of the suitable column for the pharmaceutical analysis.
Determination of Lidocaine HCl in commercially cream and injection forms by GC-FID method
Bioorganic & medicinal chemistry reports, 2022
In the present study, GC-FID method for the determination of lidocaine HCl in commercially creams and injection forms was developed and validated. The linearity of method was observed in the concentration range of 0.1-5.0 µg/mL. The accuracy (RE%) and precision (RSD%) values of the within-day and between-day of GC-FID method are less than 10.0% and 3.0%, respectively, and also limit of detection (LOD) and the limit of quantitation (LOQ) values were observed as 0.03 and 0.11 µg/mL, respectively. The analytical recovery value of lidocaine HCl was determined as 99.47% on average. As a result, it was concluded that the developed and validated GC-FID method can be easily used in routine analyzes in quality control laboratories.
Journal of AOAC International, 2010
An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150´4.6 mm, 5 mm particlesize column at 20°C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.
SEPARATION SCIENCE PLUS, 2019
A novel, simple, and rapid method for the determination of the anesthetics benzocaine and lidocaine in pharmaceutical formulations using capillary electrophoresis with spectrophotometric detection (210 nm) is proposed. In this method, the sample preparation was fast and simple, using only aqueous dilution or extraction with sonication aid. A background electrolyte composed by 10 mmol L-1 of monosodium phosphate with pH 2.1 allowed the separation of the anesthetics with good peak resolution in less than 5 min. The limits of quantification were 0.31 and 0.68 mg L −1 for lidocaine and benzocaine, respectively. The intra-and inter-day precisions (RSD%) ranged from 1.1 to 4.8% and the accuracies (as recovery percentage) varied from 84 to 109.4%. The proposed method was successfully applied for analyses of commercial samples of pharmaceutical formulations, such as otologic solution, ointment, and spray solution and lozenges for sore throat.
Simultaneous determination of tolperisone and lidocaine by high performance liquid chromatography
Journal of Pharmaceutical and Biomedical Analysis, 2001
A reversed phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of tolperisone (TP) and lidocaine (LD) has been developed. The drugs were separated on a column (4.60 × 250 mm 2 ) Spherisorb ODS (5 mm) using 5.5% triethylamine in 70/30 v/v acetonitrile/water as mobile phase 0.7 ml min − 1 and UV detection at 254 nm. The detection limits for Tolperisone hydrochloride (TP·HCl) and lidocaine hydrochloride (LD·HCl) were 0.20 ng/20 ml and 100 ng/20 ml and the quantitation limits were 0.50 ng/20 ml and 250 ng/20 ml, respectively. Linear calibration curves over the ranges of 1 -10, 10 -100 and 150-500 mg ml − 1 for TP·HCl and 10-500 mg ml − 1 for LD·HCl were established. Different calibration slopes were found for TP probably owing to changes in refractive index due to increase in TP concentration. The average recoveries of the added TP in the samples (TP·HCl tablets and injection liquid). A solutions spiked with standard TP·HCl were 99.9 and 99.7% with the RSD (n=11) of 0.66 and 0.67%, respectively. The average recovery of the added LD in the sample (injection) spiked with standard LD·HCl was 98.9% with the RSD (n=11) of 0.59%. The proposed method has been applied to the determination of TP·HCl and LD·HCl in commercial products available in Thailand. Comparative determination of TP by UV spectrophotometry and LD by colorimetry were also carried out. The results obtained by both methods were in good agreement of those obtained by the proposed method verified by using t-test. The proposed RP-HPLC method is simple, accurate, reproducible and suitable for routine analysis.