Biodegradation of cotton seed soapstocks by novel indigenous Bacillus species (original) (raw)
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Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
2016
This study was planned for the production of lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including sunflower meal, wheat bran, oat bran, rice bran and sugar cane bagasse was 23.83, 12.17, 10.40 10.00 and 16.23 U/ml, respectively. The lipase production by B. subtilis using peels of different fruits, including banana, orange, water melon and melon as carbon source, was 27.17, 21.37, 10.57 and 8.43 U/ml, respectively. The corn cob produced 12.27 U/ml while waste oils of various industries produced 16.17 U/ml (Shan oil), 13.67 U/ml (automobile), 13.37 U/ml (unbranded waste cooking oil) an...
Journal of Surfactants and Detergents, 2013
The efficacy of lipase (triacylglycerol acylhydrolases EC 3.1.1.3) as a detergent additive from a newly isolated marine halophilic bacteria Bacillus sonorensis from marine clams Paphia malabarica collected in the Kalbadevi Estuary, Mumbai, has been assessed and reported. In terms of activity and stability, the lipase exhibited maximum activity in alkaline conditions and was observed to be stable over a temperature range from room temperature to 60°C. The activity of the lipase increased in the presence of surfactants and detergents. Due to these properties of the lipase from marine bacteria, it was used as an additive in detergents to study its efficiency at corn oil removal from fabrics. The washing studies indicated that the efficiency of corn oil removal from the cotton fabrics increased by 20 % when lipase was incorporated in the detergent as compared to the treatment with detergent alone. The lipase was also capable of removing corn oil from natural as well as synthetic fabrics dyed with a respective, preferred class of dyes.
Identification and Optimization of Lipase Producing Bacteria from Palm Oil Contaminated Waste
Journal of Fundamental and Applied Sciences, 2018
Bacteria isolated from semi-solid waste, SS2B1 exhibited a greater zone of clearance, 9mm with higher lipase activity. SS2B1 isolate demonstrated a Gram-positive and rod shape arrangement under microscope observation belong to Bacillus sp based on biochemical characteristics. The effect of carbon source, nitrogen source, medium pH and temperature for the lipase production was studied. The lipase production was maximum (0.1228 µg/ml/min) at pH7, temperature 37 0 C by the lipase producing bacteria SS2B1, Bacillus sp. Increased enzymatic production was obtained when the organisms were cultured in medium supplemented with 1% tryptone and palm oil as substrate with 53.58% optimization process. The results of the present study demonstrate that the Bacillus sp. is ideal for extracellular lipase production at industrial level such as detergent, leather and fine chemical industries.
Lipase Production from Bacillus sp Using Soybean Oil Cake as Substrate
2012
Microbial lipases are holding a prominent position commercially due to their wide scope industrial and biotechnological applications. However, production cost is the major factor that limits their applicability. This indicates the need for low cost production systems. The agro wastes are nutritionally rich and can be used potentially for lipase production. In the present study 29 lipolytic bacterial isolates were obtained from oil contaminated soils of Nanded district of Maharashtra by using tributyrin agar medium. Based on quantitative studies, three isolates L5, L12 and L18 were selected to find their potential to use soybean oil cake as substrate in liquid shake culture medium for lipase production. The isolate L18 was the most active and producer strain on this medium. The isolate was identified by conventional methods as Bacillus sp. Maximum lipase yield was observed after 96 hrs of incubation (51U/mL) at 30°C temperature. The activity of partially purified lipase was studied a...
Lipases are enzymes which have several applications in many industries like pharmaceuticals, leather, detergents, and soaps, textile, food, biofuel, etc. Many organisms like bacteria, fungi, and plants are eligible to produce lipase enzyme. The present study was aimed for isolating lipase producing bacteria from lipid containing kitchen waste as samples. As lipid samples are rich in lipid and fatty acid content it makes a very good source to find microbes capable of degrading lipids by producing lipase enzymes. These isolates were identified by morphological, and biochemical characterization. The maximum lipase activity by the Bacillus cereus and Bacillus Megaterium isolates were at pH 7 at 35o C for 24 hours.
Antonie van Leeuwenhoek, 2010
An edible-oil degrading bacterial strain HH-01 was isolated from oil plant gummy matter and was classified as a member of the genus Bacillus on the basis of the nucleotide sequence of the 16S rRNA gene. A putative lipase gene and its flanking regions were cloned from the strain based on its similarity to lipase genes from other Bacillus spp. The deduced product was composed of 214 amino acids and the putative mature protein, consisting of 182 amino acids, exhibited 82% amino acid sequence identity with the subfamily I.4 lipase LipA of Bacillus subtilis 168. The recombinant product was successfully overproduced as a soluble form in Escherichia coli and showed lipase activity. The gene was, therefore, designated as lipA of HH-01. HH-01 LipA was stable at pH 4-11 and up to 30°C, and its optimum pH and temperature were 8-9 and 30°C, respectively. The enzyme showed preferential hydrolysis of the 1(3)position ester bond in trilinolein. The activity was, interestingly, enhanced by supplementing with 1 mM CoCl 2 , in contrast to other Bacillus lipases. The lipA gene seemed to be constitutively transcribed during the exponential growth phase, regardless of the presence of edible oil.
Isolation and Screening of Lipase Producing Organisms Using De-oiled Seed Cake as a Substrate
2016
Microbial enzymes are often more useful than enzymes derived from plants or animals because of the great variety of catalytic activity. Lipases are the acyl hydrolases and water soluble enzymes that play a role in fat digestion by cleaving long chain triglycerides into polar lipids. Because of an opposite polarity between the enzyme (hydrophilic) and their substrate (lipophilic), lipase reaction occurs at the interface between the aqueous and oil phase. The main purpose of the study is to utilize Seed cake for the production of industrially important enzyme. In this study the soil sample was collected from garage and petrol bunks, screened for lipase producing organisms grown on tributyrin agar media. The isolates showing maximum lipase activity were studied using de-oiled seed cake as substrate for biochemical parameters.
Plant Tissue Culture and Biotechnology, 2022
Enzyme from bacterial sources is much stable and obtained cheaply. Amylases and proteases are among the most important enzymes. During this study, indigenous amylase and protease producing bacteria were isolated from common decayed fruits and vegetable wastes viz. Papaya, Brinjal, Cucumber, Potato and Snake Gourd. Bacterial load ranged in between 0.67 × 10 9 and 9.06 × 10 9 cfu/g on NA and 1.50 × 10 9 and 7.00 × 10 9 cfu/g on PYG agar medium. Maximum mean bacterial load on both NA (6.19 ± 2.60 × 10 9 cfu/g) and PYG agar (4.54 ± 1.03 × 10 9 cfu/g) were observed in decayed papaya. A total of 113 bacterial isolates were primarily isolated. Considering better amylase and protease activity 16 isolates were selected for detailed study. The starch hydrolysis ratio (SHR) of the isolates ranged in between 1.25 ± 0.37 and 2.47 ± 0.23 while casein hydrolysis ratio (CHR) ranged in between 2.35 ± 0.12 and 6.44 ± 1.16. The highest SHR was 2.47 ± 0.23 found in Bacillus sp. of snake gourd and the highest CHR was 6.44 ± 1.16 found in Bacillus subtilis also from snake gourd. Out of the 16 isolates 13 were Gram positive and 3 were Gram negative. Gram positive isolates were identified as Bacillus acidocaldarius (4), B. firmus (2), B. lentus (4), B. subtilis (2) and B. alcalophilus (1). Gram negative isolates were identified as Edwardsiella hoshinae, Proteus myxofaciens and P. mirabilis. Six isolates having higher SHR and CHR were authenticated through molecular identification and were identified as Chryseobacterium sp. S29.2, Bacillus sp. X8, Bacillus sp. strain GA1B, Bacillus sp.TdEND26, Bacillus subtilis strain BPA28 and Bacillus subtilis BAB-881. The maximum amylase (61.33 ± 2.14 U/ml) and protease (56.91 ± 0.23 U/ml) production were observed in Bacillus sp. TdEND26 rd. In case of co-production, the highest amount of amylase (54.13 ± 1.23 U/ml) protease (81.80 ± 4.54 U/ml) production was observed in Bacillus sp. TdEND26 at 24 hrs.
Electronic Journal of Biotechnology, 2017
Background: Lipases are used in detergent industries to minimise the use of phosphate-based chemicals in detergent formulations. The use of lipase in household laundry reduces environmental pollution and enhances the ability of detergent to remove tough oil or grease stains. Results: A lipase-producing indigenous Bacillus subtilis strain [accession no. KT985358] was isolated from the foothills of Trikuta mountain in Jammu and Kashmir, India. The lipase (BSK-L) produced by this strain expressed alkali and thermotolerance. Lipase has an optimal activity at pH 8.0 and temperature 37°C, whereas it is stable at pH 6.0-9.0 and showed active lipolytic activity at temperatures 30 to 60°C. Furthermore, lipase activity was found to be stimulated in the presence of the metal ions Mn 2+ , K + , Zn 2+ , Fe 2+ and Ca 2+. This lipase was resistant to surfactants, oxidising agents and commercial detergents, suggesting it as a potential candidate for detergent formulation. BSK-L displayed noticeable capability to remove oil stains when used in different washing solutions containing buffer, lipase and commercial detergent. The maximum olive oil removal percentage obtained was 68% when the optimum detergent concentration (Fena) was 0.3%. The oil removal percentage from olive oil-soiled cotton fabric increased with 40 U/mL of lipase. Conclusions: This BSK-L enzyme has the potential for removing oil stains by developing a pre-soaked solution for detergent formulation and was compatible with surfactants, oxidising agents and commercial detergents.