Biofilm formation of Candida albicans on the surface of a soft denture-lining material (original) (raw)
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Candida Albicans Biofilm Profiles on Various Denture Base Materials
2018
The high prevalence of denture stomatitis caused by the use of dentures may influence the stability of oral candida. Assess the effects of two types of denture base material roughness on the attachment of Candida albicans. Performed roughness tests with a roughness tester and immersed dyed specimens into Eppendorf tubes containing a modified suspension of C. albicans incubated for 24 and 72 hours. The data were analyzed with a one-way analysis of variance (ANOVA) posthoc test and bivariate correlation (Pearson). The amount of C. albicans colonization on the surface of the denture base decreased when polished compared to not polished. There were differences in the number of C. albicans colonies followed by a long incubation time. The number of colony forming units of C. albicans was influenced by a smoother surface of the denture base. Clinical article (J Int Dent Med Res 2018; 11(1): pp. 191-196)
Journal of medical microbiology, 2016
Approximately 20% of the UK population wear some form of denture prosthesis, resulting in denture stomatitis in half of these individuals. Candida albicans is primarily attributed as the causative agent, due to its biofilm forming ability. Recently, there has been increasing evidence of C. albicans biofilm heterogeneity and the negative impact it can have clinically; however, this phenomenon has yet to be studied in relation to denture isolates. The aims of this study were to evaluate C. albicans biofilm formation of clinical denture isolates in a denture environment, and assess antimicrobial activity of common denture cleansers against these tenacious communities. C. albicans isolated from dentures of healthy and diseased individuals was quantified using real-time PCR and biofilm biomass assessed using the crystal violet assay. Biofilm development on the denture substratum poly (methyl methacrylate) (PMMA), Molloplast B and Ufi-gel was determined. Biofilm formation was assessed usi...
Effect of denture cleansers on Candida albicans biofilm formation over resilient liners
The journal of advanced prosthodontics, 2014
The purpose of this study was to analyze the effect of denture cleansers on Candida albicans biofilm formation over resilient liners and to evaluate compatibility between resilient liners and denture cleansers. Acrylic resin (Lucitone 199®) and 3 resilient liners (COE-SOFT™, GC RELINE™ and SOFRELINER TOUGH TOUGH®) were incubated in denture cleansers (Polident® and Cleadent®) for 8 hours a day and in unstimulated saliva for 16 hours a day (n=25/gp) for 60 days. Two-way and three-way repeated measures ANOVA were performed to compare the surface roughness (Ra), pH and C. albicans binding level by radioisotope (α=0.05). The statistical significance of the relation between Ra and adhesion was evaluated by correlation analysis. THE DEGREE OF RA WAS SIGNIFICANTLY DECREASED IN THE FOLLOWING ORDER: COE-SOFT™, acrylic resin, GC RELINE™ and SOFRELINER TOUGH®. The immersion in denture cleansers significantly increased Ra of resilient liners, except for SOFRELINER TOUGH® in Cleadent®. No signifi...
International dental journal, 2016
Denture stomatitis is the most common pathology affecting denture wearers and its main cause is colonisation of dentures with Candida albicans. This study investigated the effectiveness of two commercial composite surface sealants (Biscover(®) LV and Surface Coat(®) ) to reduce C. albicans biofilm colonisation on denture resin, as well as their surface integrity after disinfection cycles with 1% sodium hypochlorite solution. Heat-cured acrylic resin specimens were manufactured (10 mm × 10 mm × 1 mm). The specimen surfaces were mechanically polished to simulate rough or smooth denture surfaces. Four surface-treatment groups were tested: smooth surfaces [0.3 μm of mean roughness (Ra)]; rough surfaces (3 μm of Ra); rough surfaces treated with Biscover(®) LV; and rough surfaces treated with Surface Coat(®) . Specimens of each group were randomly divided to undergo immersion in distilled water or 1% sodium hypochlorite for 30 or 90 cycles each. Specimens of all groups in each immersion s...
Journal of Prosthodontic Research
To evaluate the influence of denture cleansers on the surface roughness, Candida albicans adhesion, and biofilm formation on denture base acrylic resins. Study selection: Electronic databases and gray literature were searched using an individual search strategy. In vitro studies that evaluated the effects of immersion in denture cleansers on the surface roughness (µm) and antimicrobial activity (CFU/mL) on samples of heat-polymerized denture base acrylic resins were included. Results: After screening, 17 studies were included, and a qualitative synthesis was performed. After assessing the risk of bias, only nine studies were included in the meta-analysis. The meta-analysis results showed that the evaluated solutions (0.5% sodium hypochlorite, 1% sodium hypochlorite, alkaline peroxide, and natural substances) did not influence the roughness of the acrylic resin. However, in the qualitative analysis, it was not possible to confirm an association between roughness and C. albicans adhesion and biofilm formation on the acrylic resin samples. Conclusion: Denture cleansers did not affect the surface roughness of denture base acrylic resins.
Brazilian Oral Research, 2010
This study evaluated the long-term efficacy of denture cleansers against Candida spp. biofilm recolonization on liner surface. Specimens were fabricated of a poly(methyl methacrylate)-based denture liner and had their surface roughness evaluated at baseline and after cleansing treatments. C. albicans or C. glabrata biofilms were formed on liner surface for 48 h, and then the specimens were randomly assigned to one of cleaning treatments: two alkaline peroxides (soaking for 3 or 15 min), 0.5% sodium hypochlorite (10 min) or distilled water (control; 15 min). After the treatments, the specimens were sonicated to disrupt the biofilm, and residual cells were counted (cell/mL). Long-term effectiveness of the cleaning processes was determined by submitting a set of cleaned specimens to biofilm growth conditions for 48 h followed by estimation of cell counts. The topography of specimens after cleaning treatments was analyzed by SEM. Data were analyzed by ANOVA and Tukey's test (α = 0.05). Results of cell count estimation showed significant differences in cleanliness among the treatments (p < 0.001), and it could be observed by SEM. However, no significant difference (p > 0.05) was observed among the Candida species regarding the recolonization condition. Alkaline denture cleansers showed similar cleaning performance and both differed from the control (p < 0.001). Sodium hypochlorite was the only treatment that removed biofilm efficiently, since no viable cells were found after its use. In conclusion, alkaline peroxide denture cleansers were not effective in removing Candida spp. biofilm from denture liner surfaces and preventing biofilm recolonization.