Lignans from Cell Suspension Cultures of Phyllanthus n iruri , an Indonesian Medicinal Plant (original) (raw)
Related papers
Scientific Reports
This is the first report on identification and quantification of important hepatoprotective and anticancer polyphenolic lignans such as phyllanthin (pH), hypophyllanthin (HpH), niranthin (nH) and phyltetralin (pt) in natural plant and in vitro cultures of Phyllanthus tenellus Roxb. The identification of lignans was carried out by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) and quantified using High-Performance Liquid Chromatography (HPLC). In addition, an efficient protocol has been developed for multiple shoot induction in nodal explants of in vitro derived shoots of P. tenellus. Maximum number of shoot regeneration (7.83 ± 0.15) was achieved on medium incorporated with 1.0 mg/l 6-Benzylaminopurine (BAP). The medium containing Indole-3acetic acid (IAA) 2 mg/l was superior for induction of rooting in in vitro raised shoots. The plantlets were acclimatized to the field condition with 100% survival. The quantitative HPLC analysis showed that the lignan content was variable with the auxins and cytokinins incorporated in the medium. The lignan content was higher in callus grown on Murashige and Skoog (MS) medium + 2.0 mg/l naphthaleneacetic acid (nAA). the reported protocol can be used for mass propagation and application of biotechnological approaches for improvement of P. tenellus. the results indicate intriguing possibilities for the utilization of P. tenellus plant parts as an alternative source and of callus culture to scale up bioactive lignan production for pharmaceutical applications. Phyllanthus tenellus Roxb. (Long-stalk Phyllanthus, family-Phylanthaceae) is an annual, multipurpose medicinal herb. It has been used traditionally for urolithiasis in some part of the world, and therefore is also referred to as a stone breaker 1. The herb has immunomodulatory, analgesic, anti-inflammatory, antimicrobial, antifungal, anti-hepatitis, antidiabetic and antitumor activity 1-3. In addition, the plant extract is effective for curing the kidney, urinary bladder and intestinal disorders 4,5. The phytochemical analysis showed the presence of medicinally important metabolites, such as niranthin, nirtetralin, hinoquinine and geranin 6-8. Niranthin is highly effective as anti-hepatitis B surface antigen 7. While nirtetralin and nirtetralin A, B effectively suppressed the secretion of the HBV (Hepatitis B virus); geraniin showed greater antioxidant and antihyperglycemic activities and niranthin has antiviral and anticancer activity 7,9. Phyllanthin and hypophyllanthin are the most important bioactive lignans found in Phyllanthus species. Numerous studies have proved that phyllanthin and hypophyllanthin have cardioprotective, antihepatitis, antiviral, antifibrotic, anti-inflammatory, immunomodulatory, nephroprotective and anticancer activity 10,11. To the best of our acquaintance, this is the first report on detection of medicinally important phyllanthin, hypophyllanthin and phyltetralin in P. tenellus.
Lignan Production by Cell Cultures ofLinum setaceum. andLinum campanulatum
Pharmaceutical Biology, 2005
Callus and suspension cultures of Linum campanulatum L. and Linum setaceum Brot. (Linaceae) were established to study accumulation of lignans. Justicidin B proved to be the main constituent in the callus and suspension cultures of Linum campanulatum L. Phytochemical analysis of cell cultures of Linum setaceum Brot. has demonstrated that callus produces yatein, desoxypodophyllotoxin, and b-peltatin, whereas the suspension cultures contain yatein, desoxypodophyllotoxin, b-peltatin, and 6-methoxypodophyllotoxin.
Phytochemical and Biosynthetic Studies of Lignans, with a Focus on Indonesian Medicinal Plants
In this thesis phytochemical and biosynthetic studies of lignans are described. The focus is on the Indonesian medicinal plants Phyllanthus niruri and Piper cubeba and on two Linum species, Linum flavum and L. leonii, native to European countries. Both Indonesian plants are used in jamu. Jamu is the Indonesian traditional herbal medicine, practised for many centuries in the Indonesian community to maintain good health and to treat diseases. The manufacturing of jamu is shifting more and more from household scale to the bigger industries. As the economical and clinical value of jamu nowadays increases in Indonesia, there is a need for further scientific proof and well conducted research. Jamu has to be developed in order to assure its efficacy and safety. Chapter 2 reviews the research carried out on jamu and jamu plants, covering a broad range of aspects including phytochemistry, pharmacology, toxicologicy and clinical studies. In addition, ethical issues such intellectual property ...