Quantitative Analysis of Clathrin-Mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy (original) (raw)

Methods in molecular biology, 2018

Abstract

The budding yeast Saccharomyces cerevisiae has provided a useful model for studying clathrin-mediated endocytosis due to ease of genetic manipulation and crosssectional imaging of individual endocytic sites. This protocol describes a method for using live cell fluorescence microscopy to analyze clathrin-mediated endocytosis and the contributions of actin to the process.

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