Sox-9 transient transfection enhances chondrogenic expression of osteoarthritic human articular chondrocytes in vitro: preliminary analysis (original) (raw)

Tissue Engineering and Regenerative Medicine, 2011

Abstract

In this study, we are taking step to actively manage osteoarthritis that may help gain control over osteoarthritic pain and delay the degenerative changes in articular cartilage in future. We transiently over expressed cartilage transcriptional factor, human sox-9 gene in chondrocytes derived from consented osteoarthritic patients after joint surgery. The expression vector carrying human sox-9 gene, pAdTrack-sox9 was transformed into One Shot TOP 10 Chemically Competent E. coli according to the manufacturer protocol. Plasmid purification was performed in accordance with QIAGEN (R) plasmid purification kit procedure. We compared the efficiency between two transfection techniques i.e. lipofection using Lipofectamine (TM) 2000 kit from Invitrogen, USA and nucleofection using Human Chondrocytes Nucleofector (R) kit from Amaxa Biosystem, Germany. Chondrocytes were cultured and transfected with sox-9 gene at passage 1 according to the manufacturers' protocols. Transfected chondrocytes...

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