Survey differentiation of mesenchymal stem cell to chondrocyte using synovial fluid and coldatmospheric plasma treated nanofibrous pcl substrate (original) (raw)

MOJ Biology and Medicine, 2021

Abstract

Tissue engineering has developed strategy for the repair and regeneration of a variety of tissues. The demand for effective treatment strategies of cartilage cause to autologous human Mesenchymal stem cells (hMSC) used for the repair of cartilage defects. In this study effect of some factors on human MSCs for differentiation to chondrocyte were surveyed such as Present of Poly Ɛ-caprolactone (PCL) nanofibrous scaffold and cold atmospheric plasma (CAP), also effect and amount of synovial fluid were evaluated. In this experimental study cell culture for differentiation assay performed in 6 well pellets by DMEM high glucose medium with a supplement of 10% FBS and synovial fluid 5% and 10% of medium add to pellets for 21 days. Total cellular RNA extracted was used for analysis by RT-PCR. Alcian blue staining confirmed the chondrogenic differentiation. MTT assay results in this study showed that the cells proliferation gradually increased from 1st day to 21st days on the electrospun nano...

mohammad fazilati hasn't uploaded this paper.

Let mohammad know you want this paper to be uploaded.

Ask for this paper to be uploaded.