A preliminary study on electrochemical biosensors for the determination of total cholinesterase inhibitors in strawberries (original) (raw)

This preliminary study presents a novel approach for determining total cholinesterase inhibitors in strawberries using electrochemical biosensors. The focus is on the application of amperometric detection of acetylcholinesterase (AChE) activity through the measurement of hydrogen peroxide oxidation. The developed method showcases high sensitivity and reproducibility, as well as reduced analysis time, by incorporating a double-enzymatic sensor setup. The effectiveness of the biosensor was validated with the pesticide Paraoxon, demonstrating a calibration plot with a concentration range of 0-21 ng mL and a limit of detection of 4.7 ng mL.