Evaluation of a Qualitative Antibody Detection Method of Typhoid Fever (original) (raw)
Related papers
2014
Typhoid fever still continues to be a major public health problem, particularly in many developing countries. A simple, reliable, affordable and rapid diagnostic test has been a long-felt need of the clinicians. We, therefore, prospectively evaluated the sensitivity and specificity of Typhidot (IgM), a serological test to identify IgM antibodies against Salmonella typhi. The study was carried out in the department of Microbiology, Mymensingh Medical College, Mymensingh between June, 2006 and July, 2007, on a total of 100 samples from clinically suspected patients to have typhoid fever. Blood culture as well as Typhidot test were performed for each of the cases. Out of 100 clinically diagnosed typhoid fever, 14 were blood culture positive for S. typhi and 73 were Typhidot (IgM) positive. Among 14 culture positive cases, 13 (92.85%) were Typhidot (IgM) positive. The test was also positive in 04 (20%) out of 20 febrile controls. None of the healthy controls was positive by Typhidot (Ig...
Evaluation of Typhidot (IgM) for Early Diagnosis of Typhoid Fever
Bangladesh Journal of Medical Microbiology, 2009
Typhoid fever still continues to be a major public health problem, particularly in many developing countries. A simple, reliable, affordable and rapid diagnostic test has been a long-felt need of the clinicians. We, therefore, prospectively evaluated the sensitivity and specificity of Typhidot (IgM), a serological test to identify IgM antibodies against Salmonella typhi. The study was carried out in the department of Microbiology, Mymensingh Medical College, Mymensingh between June, 2006 and July, 2007, on a total of 100 samples from clinically suspected patients to have typhoid fever. Blood culture as well as Typhidot test were performed for each of the cases. Out of 100 clinically diagnosed typhoid fever, 14 were blood culture positive for S. typhi and 73 were Typhidot (IgM) positive. Among 14 culture positive cases, 13 (92.85%) were Typhidot (IgM) positive. The test was also positive in 04 (20%) out of 20 febrile controls. None of the healthy controls was positive by Typhidot (IgM). The sensitivity, specificity, positive predictive value and negative predictive value of the test using blood culture as gold standard were 92.85%, 90.00%, 76.47% and 97.29% respectively for typhoid fever. Typhidot
Aim: To assess three business typhoid fast immunizer tests for Salmonella Typhi antibodies in cases associated with typhoid fever in Lahore, Pakistan. Methods: The analytic exactness of Remotest TUBEX® and Typhoid was evaluated against that of blood culture. Execution was demonstrated for situations through pretest probabilities of 7% and half. Our current research was conducted at Jinnah Hospital, Lahore from May 2018 to April 2109. Results: In all out 95 patients enlisted: 54 (58.7%) from Pakistan and 39 (43.6%) from the United Republic of Tanzania. Salmonella Typhi was segregated from the blood of 32 (35.6%) cases. The semiquantitative slide agglutination and single-tube Widal tests had positive prescient qualities (PPVs) of 27.2% (96% certainty span, CI: 0.7-81.7) and 24.1% (96% CI: 4.7-57.8), separately. The more current typhoid quick immune response tests had practically identical PPVs: TUBEX®, 55.2% (96% CI: 37.8-72.7); Typhoid IgM, 57.8% (96% CI: 38.5-77.6); and Typhoid IgG, 56.4% (96% CI: 38.7-72.3). For the pretest likelihood of 6%, PPVs were: TUBEX®, 12.1% (96% CI: 4.7-19.8); Typhoid IgM, 8.2% (96% CI: 5.7-17.2); and Typhoid IgG, 11.0% (6.3-18.4). For a pretest likelihood of half, PPVs remained: TUBEX®, 71.3% (96% CI: 58.4-81.6); Typhoid IgM, 65.6% (95% CI: 54.0-75.6); and Typhoid IgG, 71.1% (96% CI: 57.1-82.2). Conclusion: Semiquantitative slide agglutination and single-tube Widely tests were done ineffectively. TUBEX® and Typhoid might remain reasonable once pretest likelihood is high and blood societies are inaccessible, however their exhibition doesn't legitimize sending in routine consideration settings in Pakistan.
Rapid Diagnosis of Typhoid Fever-A Comparative Study of Typhidot and Widal Test
International Journal of Bioassays, 2014
Typhoid fever is a major public health problem in Asia Pacific region. It is endemic in Indian subcontinent. Conventional methods for its diagnosis are blood culture and Widal test. Typhidot is a new rapid serological test which is now commercially available and reliable in diagnosis of typhoid fever even with limited resources. The study included 100 patients of all age groups who presented with fever in the outpatient departments (OPD). Blood culture, widal test and typhidot test were performed in all the patients. Typhidot test is an immunodot ELISA which detects outer membrane protein specific for Salmonella typhi within an hour. It separately identifies IgM and IgG antibodies. Typhidot and widal tests results were then compared in clinically suspected cases of typhoid fever (62 Group-1) and non typhoidal fever (38 Group-2). Seven (11.29%) patients of Group-1 were blood culture positive, 45(72.58%) were widal test positive and 52 (83.8%) were positive in typhidot test. All patie...
Serology of Typhoid Fever in an Area of Endemicity and Its Relevance to Diagnosis
2001
. At a specificity of >0.93, the sensitivities of the different tests were 0.75, 0.55, and 0.52 for the anti-LPS IgM, IgG, and IgA ELISAs, respectively; 0.28 for the antiflagellum IgG ELISA; 0.47 and 0.32 for the Widal TO and TH tests, respectively; and 0.77 for the anti-serotype Typhi IgM dipstick assay. The specificity of the IDeaL TUBEX was below 0.90 (sensitivity, 0.87; specificity, 0.76). The serological assays based on the detection of IgM antibodies against either serotype Typhi LPS (ELISA) or whole bacteria (dipstick) had a significantly higher sensitivity than the Widal TO test when used with a single acute-phase serum sample (P < 0.007). These tests could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever but are culture negative or in regions where bacterial culturing facilities are not available.
International Journal of Biosciences (IJB), 2014
Typhoid fever, caused by Salmonella Typhi (S. Typhi), is one of the deadly diseases and is a serious public health problem around the world. The prevalence of typhoid fever in five flood affected Districts (Bannu, Hangu, Karak, Kohat and Peshawar) of khyber Pakhtunkhwa province of Pakistan during 2010/11 was investigated in this study. The blood samples of 110 hospitalized patients were used for clinical diagnosis applying widal test and Dot immunosorbent Assay (DIA), and their results were compared for analysis. A total of 60 patients were found positive for widal test, however only 67 % patients of them were positive for DIA. More males (52%) than females (48%) were infected by Salmonella. The ratio of typhoid patients was greater in young people having ages between 24-38 years. These preliminary informations could be helpful for future mass screening in the country.
Journal of Health and Allied Sciences NU, 2013
Background: Typhoid fever, caused by Salmonella enterica serotype Typhi, is endemic in the Indian sub-continent including Bangladesh, South-east and Far-east Asia, Africa and South Central America. The disease can occur in all age group with highest incidence among children. Blood culture is regarded as the gold standard for diagnosis and carry 70-75% diagnostic yield in the first week of illness. However, this requires laboratory equipment and technical training that are beyond the means of most primary health care facilities in the developing world. Typhidot is a rapid dot-enzyme immune assay (EIA), which detects IgG and IgM antibodies to a specific 50 kD outer membrane protein (OMP) antigen of Salmonella enterica serotype Typhi. Typhidot becomes positive as early as in the first week of fever. The results can be visually interpreted and is available within one hour. Materials and method: Fifty blood samples, collected aseptically from patients clinically diagnosed of Typhoid feve...
The Journal of infection, 2017
The diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi (S. Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients. IgM against 12 purified antigens and the Vi polysaccharide was measured by ELISA in plasma from patients with confirmed typhoid fever (n=32), other confirmed infections (n=17), and healthy controls (n=40). ELISAs with the most specific antigens were performed on plasma from 243 patients with undiagnosed febrile disease. IgM against the S. Typhi protein antigens correlated with each other (rho>0.8), but not against Vi (rho<0.6). Typhoid patients exhibited higher IgM against 11/12 protein antigens and Vi than healthy controls and those with other infections. Vi, PilL, and CdtB exhibited the greatest sensitivity and specificity. Specificity and sensitivity was improved when Vi was combined with a protein antigen, generating sensitivities and s...
Validity of Immunoglobulin M Anti Salmonella typhi Serologic Test in Childhood Typhoid Fever
Althea Medical Journal, 2017
Background: Typhoid fever, which mostly affects children, remains a major health problem in developing countries. Early diagnosis will help the management and thus, reduce morbidity and mortality. However, a rapid diagnostic test that detects the presence of immunoglobulin M (IgM) directed towards Salmonella typhi (S. typhi) antigen remains controversial despite its popularity. This study was aimed to assess the validity of IgM anti S. typhi serologic test in childhood typhoid fever. Methods: This retrospective diagnostic test, used blood culture as gold standard. Forty-one typhoid fever children with fever of 1-14 days admitted to Dr. Hasan Sadikin General Hospital Bandung from 2013 to 2015 were recruited. Diagnosis of typhoid fever is made clinically. Data were analyzed by Receiver Operating Characteristic (ROC) curve and diagnostic test. Results: Forty one children diagnosed with typhoid fever, 37 were positive for IgM anti S. typhi, but only 18 were positive for S. typhi in blood culture. IgM anti S. typhi (cut-off ≥4) test had an Area Under the Curve (AUC) of 59%, sensitivity of 100% and specificity of 17.39%. IgM anti S. typhi with cutoff >8 showed the highest AUC with sensitivity of 55.56% and specificity of 73.68%. Conclusions: IgM anti S. typhi test of cutoff >8 performs better than cutoff ≥4 in terms of AUC..