The effect of polyphenol to visceral fat profile protein at obesity model rat (original) (raw)
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International Journal of PharmTech Research
Objective of this study are toassessthe effect of polyphenol from Rambutan Peel Extract (RPE) on protein profile and serum lipid in normal and obesity rat model. Normal and obesity rat model have been treated with polyphenol from rambutan peel extract for 12 weeks. Rat were divided into 2 major groups based on their weight which normal rat and obesity model rat respectively have average weight 180-200g and 360-380g. This treatment were divided into minor groups which were placebo (without treatment), treatment with ellagic acid and polyphenol (dosage 15 mg/kg BW; 30 mg/kg BW and 60 mg/kg BW). Polyphenol were delivered by oral administration for 12 weeks. Protein profile on visceral fats wereidentified using SDS page and serum lipid wasevaluated based onbody weight, weight on visceral fat andtriglycerides. Polyphenol were significantly inhibit body mass gain on treatment polyphenol 30mg/kg BW. Mass of visceral fats were not different on treatment and non-treatment rat, however significantly different on normal and obesity rat model. Level of triglycerides were less on rat treated with polyphenol 30mg/kg BW.Protein profile characteristic normal rat and obesity-model rat were in range between117-20 kDa. The amount of band protein were found in normal rat were less than the amount of protein on obesityrat model. There was difference molecular weight at protein density 57kDa for obesity rat model which has been treated with polyphenol. Polyphenol from RPEdecreased triglyceride level and amount of protein band on induced high calorie diet rat.
Effect of Plant Polyphenols on Adipokine Secretion from Human SGBS Adipocytes
Biochemistry research international, 2011
Introduction. Adipose tissue contributes to atherosclerosis with mechanisms related to adipokine secretion. Polyphenols may exhibit antiatherogenic properties. The aim of the study was to investigate the effects of three polyphenols, namely, quercetin, epigallocatechin gallate (EGCG), and resveratrol on adipokine secretion from cultured human adipocytes. Methods. Human SGBS adipocytes were treated with quercetin, EGCG, and resveratrol for 24 and 48 hours. Visfatin, leptin, and adiponectin were measured in the supernatant. Results. Visfatin secretion was inhibited by quercetin 10 μM by 16% and 24% at 24 and 48 hours respectively. The corresponding changes for quercetin 25 μM were 47% and 48%. Resveratrol 25 μM reduced visfatin by 28% and 38% at 24 and 48 hours. EGCG did not have an effect on visfatin. None of tested polyphenols influenced leptin and adiponectin secretion. Conclusion. Quercetin and resveratrol significantly decreased visfatin secretion from SGBS adipocytes. This effec...
doi:10.1155/2011/285618 Research Article Effect of Plant Polyphenols on Adipokine Secretion from
2013
Copyright © 2011 Christos S. Derdemezis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. Adipose tissue contributes to atherosclerosis with mechanisms related to adipokine secretion. Polyphenols may exhibit antiatherogenic properties. The aim of the study was to investigate the effects of three polyphenols, namely, quercetin, epigallocatechin gallate (EGCG), and resveratrol on adipokine secretion from cultured human adipocytes. Methods.HumanSGBS adipocytes were treated with quercetin, EGCG, and resveratrol for 24 and 48 hours. Visfatin, leptin, and adiponectin were measured
The physiological response of obese rat model with rambutan peel extract treatment
Asian Pacific Journal of Tropical Disease, 2014
Objective: To determine body weight gain, expression of Igf-1 and Igf-1 receptor on obese rat model treated with rambutan peel extract (RPE) as a physiological response. Methods: Normal and obese rat feed with normal and high calorie diet around 12 weeks and continued to treat with ellagic acid, RPE 15, 30 and 60 mg/kg body weight respectively. Physiological responses observed were weight gain and expression of Igf-1 with its receptor. Body weight of rat was weighed once per week. Expression of Igf-1 and igf-1R observed with fluorescence immunohistochemistry. The intensity of Igf-1 and Igf-1R expression was analysis using FSX-BSW software. Results: The lowest weight gain was obtained on obese rat model treated with RPE 30 mg/kg body weight. The expression of Igf-1 and Igf-1R were reduced on obese rat model treated with RPE compared with obese rat model of non treatment (P<0.05). The low expression of Igf-1 and Igf-1R was found on obese rat model treated with ellagic acid and RPE 30 mg/kg body weight. Conclusions: The RPE was effecting to the physiological response on obese rat model. The RPE 30 mg/kg body weight inhibited body weight gain and decreased the expression of Igf-1 and Igf-1R of obese rat model.
Food Research International, 2014
Polyphenols generally show beneficial health effects upon supplementation in diet-induced obese rodent models, including reduced body weight gain and reduced levels of markers for cardiovascular diseases (CVD). However, there appear to be large differences between studies, which might be due to differences in models, strains, dietary background, or even concentration of polyphenol that is used. Therefore, we performed a systematic phenotypic evaluation of the effects of selected polyphenols in wildtype C57BL/6JOlaHsd mice. Epigallocatechin-gallate, quercetin, and resveratrol, representing three different phenolic classes, were each added in equimolar amounts (0.50% (w/w), 0.33%, and 0.25%, respectively) to a purified moderate high fat (30 energy%) diet for 12 weeks. We studied the polyphenol-induced physiological and molecular effects between them and relative to the non-supplemented control group during and at the end of the nutritional intervention. Results showed that these polyphenols were present in circulation, but did not induce beneficial health effects as analyzed by oral glucose tolerance testing or serum adipokines and CVD-markers such as vascular adhesion molecules. Remarkably, transcriptomics of white adipose tissue showed overlapping sets of significantly differential transcript levels between these polyphenols; AMPK and Notch signaling were affected by these polyphenols. However, mitochondrial processes and mitochondrial density in this tissue did not differ between the polyphenols, which suggested that there was no direct effect on adipose tissue.
Anti-Obesity Effects of Polyphenol Intake: Current Status and Future Possibilities
International Journal of Molecular Sciences, 2020
The prevalence of obesity has steadily increased worldwide over the past three decades. The conventional approaches to prevent or treat this syndrome and its associated complications include a balanced diet, an increase energy expenditure, and lifestyle modification. Multiple pharmacological and non-pharmacological interventions have been developed with the aim of improving obesity complications. Recently, the use of functional foods and their bioactive components is considered a new approach in the prevention and management of this disease. Due to their biological properties, polyphenols may be considered as nutraceuticals and food supplement recommended for different syndromes. Polyphenols are a class of naturally-occurring phytochemicals, some of which have been shown to modulate physiological and molecular pathways involved in energy metabolism. Polyphenols could act in the stimulation of β-oxidation, adipocyte differentiation inhibition, counteract oxidative stress, etc. In thi...
2017
Obesity is a serious problem and a solution is needed for its treatment and management. Natural products which are more targeted offer an alternative therapy. Objective of the study is to determine the protective effects of a new Poly Herbal Formulation (PHF) against High Fat Diet (HFD) induced systemic oxidative stress in female wistar rats. Detailed chemical investigation was carried out for the polyphenolic rich PHF by preparative HPTLC fingerprinting. Obesitic parameters including anthropometric, physiological and biochemical parameters were evaluated in vivo using female wistar rats. The results of HPTLC preparative fingerprinting analysis chromatogram confirmed the presence of polyphenols such as curcumin and plumbagin in the PHF. In vivo results revealed significant reduction in body weight, BMI, abdominal circumference, lipid profile and levels of enzymic antioxidant values in treatment groups (p < 0.05). The present study suggests that the new PHF has antiobesity effects...
Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets
Lipids, 1992
Male Wistar rats were fed a high fat diet (HFD) containing 2.5% cholesterol and 16% lard supplemented with polyphenolic natural products namely quercetin, morin or tannic acid (100 mg/rat/day) for 4, 7 and 10 wk. Rats fed HFD without the supplements served as control. The effects of these compounds on blood lipid profiles, enzymes, liver fat and aorta of the rat were studied. In rats fed HFD containing tannic acid, plasma total cholesterol (TC), low density lipoprotein cholesterol (LDLC) and triglyceride (TG) were reduced by 33.3%, 29.6% and 65.1%, respectively, at week 10. High density lipoprotein cholesterol (HDLC) concentration was not altered. Fat deposition was also decreased in the liver of these rats. Morin significantly reduced plasma TG (65.1%) and liver fat only at week 7 while at week 10 it reduced plasma TC and LDLC by 30.9% and 29.3% respectively. The plasma HDLC concentration was increased by 47.3% at week 4 but no effect was seen at weeks 7 and 10. In the rats fed HFD containing quercetin, plasma HDLC was increased by 28.6% at week 7 but at week 10, plasma LDLC was increased by 21.2%. Quercetin did not cause any significant changes on the plasma TC, TG and liver fat at weeks 4, 7 and 10. Plasma alanine aminotransferase, alkaline phosphatase and bilirubin in control and treated groups were not significantly different. However, hepatic lipase activity in rats fed tannic acid was significantly lower. Aortae of all groups of rats showed no abnormalities. The present report indicates that tannic acid and morin are effective in reducing plasma and liver lipids when supplemented with a high fat diet in rats.
Investigation of Effect of Polyherbal Mixtures on Cafeteria Diet Induced Obesity in Wistar rats
Current research in diabetes & obesity journal, 2022
Background: To evaluate the anti-obesity activity of polyherbal mixture of selected herbs in cafeteria diet induced obesity in Wistar rats. Method: Hydro alcoholic extracts of herbal plant Annona muricatta, Momordica charantia, Vaccinium uliginosum were purchased and authenticated from Green Heaven India. The Acute Oral Toxicity study of polyherbal mixture was performed according to OECD guideline 423.The antiobesity effect of polyherbal mixture was evaluated in cafeteria diet (CD) induced obesity. The polyherbal mixture (PHM) at two different dose level (200 mg/kg and400 mg/kg BW) was administered orally for a period of 35 days and various parameters of anti-obesity were estimated. Result: The half maximal inhibitory concentration (IC50) values were estimated for combination of PHM in in vitro for antilipase activity. Oral administration of PHM for 35 days significantly decreases in calorie intake, bodyweight, cholesterol level and fasting glucose level. It showed that there is significant increase in parameters of liver function, BMI and Nasoanal length and level of white adipose tissue. Conclusion: The dose of 200 mg/kg and 400 mg/kg of PHM significantly reduced body weight and calorie intake indicating its antiobesity effect. The mixture of PHM at both doses effectively improved serum lipids level and decreased serum blood glucose level suggesting its effect on carbohydrate and lipid metabolism. The decreased AST and ALT level signifies that the PHM do not interferes in liver function. The histopathological studies of liver tissue from PHM treated animals showed improvement in fatty liver and hepato-steatosis as compared to control group. From all above findings it is concluded that the PHM produces significant antiobesity effect in CFD induced obesity in Wistar rats at doses 200 & 400 mg/kg.