Heterodera latipons on barley in Jordan (original) (raw)
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Journal of Plant Diseases and Protection, 2015
Cereal cyst nematodes (CCN) are important plant-parasitic nematodes of wheat and barley, and exist in most of the cereal growing regions of the world. As there is limited information on the nature and distribution of CCN species in Syria, a survey was conducted in northeastern Syria to assess the distribution of CCN in the main wheat and barley growing areas. In the summer of 2009, a total of 167 composite soil samples were collected from 167 wheat and barley fields. Cysts were extracted from soil using the Fenwick can technique, then quantified and identified up to the species level by using both morphological and molecular methods. The study revealed that 62% of the fields were infested with the three Heterodera species: H. avenae, H. filipjevi and H. latipons. However, the most prevalent species was H. latipons, which was present in 76% of the infested samples, while it occurred alone in 67% of those samples. Heterodera avenae was detected singly in 20% of the samples, while 11% consisted of mixed populations with other species. Heterodera filipjevi was never detected alone; it was found mixed with H. avenae and/or H. latipons in 9% of the samples. The high number of extracted cysts (up to 116 cysts × 200 g-1 of soil) is most likely related to the monoculture practiced in that region and the fact that growers are unaware of the existence of resistant varieties.
International Journal of Agriculture and Biology
Cereal cyst nematodes are one of the most important soil-borne pathogens of cereals throughout the world. This group of nematodes is considered the most economically damaging pathogens of wheat and barley in Iran. In the present study, a series experiments were conducted during 2007-2010 to determine the distribution and population density of cereal cyst nematodes and to examine the biology of Heterodera latipons in the winter wheat cv. Sardari in a microplot under rain-fed conditions over two successive years in Markazi province in central Iran. Results of field survey showed that 40% of the fields were infested with at least one species of either Heterodera filipjevi or H. latipons. H. filipjevi was most prevalent in Farmahin, Tafresh and Khomein, with H. latipons being found in Khomein and Zarandieh regions. Female nematodes were also observed in Bromus tectarum, Hordeum disticum and Secale cereale, which are new host records for H. filipjevi. Also, H. filipjevi and H. latipons w...
Incidence of Cereal Cyst Nematodes (Heterodera avenae type B and H. filipjevi) in southwestern Iran
A survey on cereal fields of Khuzestan province during 2008-2011 revealed that cereal cyst nematodes (CCNs) are widely distributed in this region. The CCNs were present in 37 and 35% of the 200 samples collected from wheat and barley fields respectively. The species were identified as Heterodera avenae type B and H. filipjevi which the morphological and morphometric identification were confirmed by rRNA-ITS RFLP. Population density of CCNs ranged from 2 to 103 cysts (mean 18)/100 g of dried soil with an average of 395 (0-3400) J2 and eggs in wheat samples. Whilst the number of cysts in barley samples were counted 3-71 cysts (mean 11) /100 g soil, the J2 and eggs averaged 166 (0-900). The lowest and the highest rate of infestation were observed in the regions of Ahvaz and Behbahan with 8 and 83 % respectively. The number of J2 and eggs of CCNs in some regions were greater than damage threshold level considered for the CCNs and it is likely they could cause economic yield loss in thes...
Incidence of Cereal Cyst Nematodes (Heterodera avenae type B
2016
Abstract: A survey of cereal fields of Khuzestan province during 2008-2011 revealed that cereal cyst nematodes (CCNs) are widely distributed in this region. The CCNs were present in 37 and 35 % of the 200 samples collected from wheat and barley fields respectively. The species were identified as Heterodera avenae type B and H. filipjevi the morphological and morphometric identifications of which were confirmed by rRNA-ITS RFLP. Population density of CCNs ranged from 2 to 103 cysts (mean 18)/100 g of dried soil with an average of 395 (0-3400) J2 and eggs in wheat samples. Whilst the number of cysts in barley samples were counted 3-71 cysts (mean 11) /100 g soil, the J2 and eggs averaged 166 (0-900). The lowest and the highest rates of infestation (8 and 83%) were observed in the regions of Ahvaz and Behbahan respectively. The number of J2 and eggs of CCNs in some regions were greater than the damage threshold level considered for CCNs and it is likely they could cause economic yield ...
Turkish Journal of Entomology, 2020
The Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) is a destructive plant-parasitic nematode on cereal crops in particularly wheat and barley. It has a global distribution with a severe negative impact on yield quantity. In this study, a survey was conducted to identify plant-parasitic nematodes in cereal-growing areas in Cyprus. Forty-five samples including roots and soil from the root zone of plants were collected from cereal fields located in Gazimağusa, Girne, Güzelyurt and Lefkoşa Provinces before crop harvesting from late-May and early-June in 2017. Cyst-forming nematodes were determined by Fenwick's flotation and decanting techniques from 37 soil samples (82%). The internal transcribed spacer (ITS) regions of the ribosomal DNA of isolates were amplified and sequenced and subjected to a BLASTn search of the NCBI database for species identification, and the analyses showed that all samples were identified as H. latipons. Phylogenetic analyses based on ITS sequences revealed that H. latipons isolates from Northern Cyprus were closely related to isolates obtained from Morocco, Russia, Syria and Turkey. Data of this study demonstrated for the first time the presence of H. latipons in the cereal fields of Gazimağusa, Girne, Güzelyurt and Lefkoşa Provinces, where the nematode most likely causes serious economic problems in the cereal production. These results were the most up-to-dated analyses on the occurrence of H. latipons in cereal fields of Northern Cyprus and provided basic data for breeding programs to improve the resistant levels in the local cultivars.
Journal of Agricultural Science and Technology, 2017
To identify the Cereal Cyst Nematodes (CCN) and their distribution and population density in wheat fields in Chaharmahal and Bakhtiari Province based on interpolation by geographic information system, a total of 212 composite soil and root samples were randomly collected from different wheat fields of nine districts of the province during 2013-2014. The soil samples were explored for number of cysts, second-stage Juveniles (J2) and eggs. The species were identified based on morphological, morphometric and molecular characteristics. Analysis was performed by Arc GIS software using interpolation technique for determination of raster map. The results showed that 42% of soil samples were infested with Heterodera filipjevi containing an average of 994 eggs and J2 per 200 g of soil. The maximum infestation was observed in Dehsahra region in Lordegan district with an average of 4720 eggs and J2 per 200 g soil. Wheat fields of Kohrang, Kiyar and Farsan districts showed low population densit...
TURKISH JOURNAL OF ZOOLOGY, 2018
The Mediterranean cereal cyst nematode, Heterodera latipons, is an important plant parasitic nematode that negatively impacts cereal production worldwide, particularly in the Mediterranean Basin. Successful control of cereal cyst nematodes requires knowledge of the nematode species/pathotypes and their biology. However, little information is currently available concerning the phenotypic and genotypic variability of H. latipons populations in Turkey. This study analyzed the morphological/morphometric and genetic characteristics of Turkish H. latipons populations to define intraspecific distinctions between them. Morphological and morphometric studies focused on the pattern of the perineal region of cysts and second stage juveniles (J2), while the molecular analyses used sequenced ITS-rDNA regions targeting the internal transcribed spacer (ITS) region of rDNA. The data from these investigations demonstrate that nematode populations from Adana and Hatay provinces differ from the populations collected in Kilis, Gaziantep, and Mardin provinces. These phenotypic and genotypic differences between H. latipons populations may indicate that they are heterogenic, with at least two pathotypes. To date, this study is the most comprehensive analysis identifying H. latipons populations in major wheatproducing areas of Turkey.
Two assays were carried out in Badjgah, Fars province, the south of Iran, with the purpose of establishing the development time of Heterodera filipjevi life cycle phases in wheat roots and also to study the effect of the certain cereals including bread wheat, durum wheat, barley, corn and millet on the nematode population densities. Results of field microplots showed that hatching of eggs was occurred at 2-6 °C. The second stage juveniles (J2) were observed in the roots within periods with 13 °C average temperature and with no precipitation. Juveniles (J3) and (J4) were appeared within the roots at 110 and 120 days after penetration, respectively. The white females adhered to the roots and males in soil were found 7 and 14 days later, respectively. Cyst formation occurred about two months after observing white females; In short, the process of H. filipjevi development was completed during 197 days. Results of greenhouse assay showed that the studied nematode species reproduced best ...
2020
The cyst nematode Heterodera avenae Wollenweber, 1924 (Heteroderidae) is an obligate endoparasite of grasses. It is the most studied and most damaging nematode known for more than a century. The study of the distribution of the H. avenae cyst nematode was carried out on some plots of cereal-oriented municipalities in the Tiaret region to assess the infestation levels of the plots in order to study the impact of the nematode infestation on cereal yields. Spearman’s test was used, which allowed us to study the correlation between the degree of infestation and cereal yields, particularly wheat, barley and oats. The plots of the communes surveyed are all infested by H. avenae with different levels of infestation except the plots of the town of Sebaine and Mahdia where the infestation is practically non-existent (0 cysts/100 g of soil). The correlation is negative between the degree of infestation and the yield (r = -0.06), the yields of hard wheat are low when the degree of infestation ...