Upstream non-coding region which confers polar expression to Ri plasmid root inducing gene rolB (original) (raw)
MGG Molecular & General Genetics, 1989
Abstract
Root differentiation could be elicited on carrot discs by transformation with the agropine Ri plasmid rolB gene cloned in the binary vector Bin19, provided two conditions were met. Firstly, an adequate auxin supply had to be provided. This was achieved by co-inoculation with a strain carrying only the auxin synthetic genes of the TR-DNA. Most of the resulting roots were
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