Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria (original) (raw)
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Journal of Microbial & Biochemical Technology, 2014
Sponges are host organisms for various symbiotic microorganisms such as Archea, Bacteria, Cyanobacteria and Microalgae. Sponges associated microorganisms are sources of wide variety of useful natural products like antibiotics, anti-inflammatory, antioxidant, antiviral, antifouling and cytotoxic compounds. Nearly 60 isolates of action bacteria were found to be associated with 6 species of marine sponges collected at various locations. The isolates were screened for antimicrobial activity against 6 pathogenic bacteria and 4 pathogenic fungi. Among 60 isolates, 15 isolates showed antibacterial activity and 6 isolates showed antifungal activity. Among active isolates, isolate no.42 showed highest antimicrobial activity against all the pathogenic bacteria and fungi studied and it was identified as streptomyces species.
Cultivable Sponge-Associated Actinobacteria from Coastal Area of Eastern Mediterranean Sea
Advances in Microbiology, 2014
In this study, 16 marine sponge samples collected from Eastern Mediterranean Sea were used for the isolation of sponge-associated Actinobacteria. Different media and pre-treatment methods were applied for the isolation experiments. In total, 7578 actinomycetes colonies were observed on the primary isolation plates, and 20 representative isolates were selected for further studies based on their morphologies and chemical profiles. These isolates were investigated for their antimicrobial activities, seawater requirements and 16S rRNA gene sequences. The phylogenetic analysis revealed that 80% of the isolated Actinobacteria was a member of the Streptomyces genus. All the isolates showed no absolute requirement for seawater. Fifteen of the selected isolates exhibited antimicrobial activity against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus faecium, whereas 3 isolates were active against Candida albicans.
Cultivable Sponge-Associated Actinobacteria from Coastal Area of Eastern Mediterranean
2016
In this study, 16 marine sponge samples collected from Eastern Mediterranean Sea were used for the isolation of sponge-associated Actinobacteria. Different media and pre-treatment methods were applied for the isolation experiments. In total, 7578 actinomycetes colonies were observed on the primary isolation plates, and 20 representative isolates were selected for further studies based on their morphologies and chemical profiles. These isolates were investigated for their an-timicrobial activities, seawater requirements and 16S rRNA gene sequences. The phylogenetic analysis revealed that 80 % of the isolated Actinobacteria was a member of the Streptomyces genus. All the isolates showed no absolute requirement for seawater. Fifteen of the selected isolates ex-hibited antimicrobial activity against methicillin resistant Staphylococcus aureus and vancomycin
Marine Drugs, 2010
Terrestrial actinomycetes are noteworthy producers of a multitude of antibiotics, however the marine representatives are much less studied in this regard. In this study, 90 actinomycetes were isolated from 11 different species of marine sponges that had been collected from offshore Ras Mohamed (Egypt) and from Rovinj (Croatia). Phylogenetic characterization of the isolates based on 16S rRNA gene sequencing supported their assignment to 18 different actinomycete genera representing seven different suborders. Fourteen putatively novel species were identified based on sequence similarity values below 98.2% to other strains in the NCBI database. A putative new genus related to