Bioremediation Potentials of Bacteria Isolated from Rhizosphere of Some Plants of Oil Contaminated Soil of Niger Delta (original) (raw)
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The synergistic approach of plants and rhizobacteria in crude oil contaminated soil in three different locations were carried out. The presence of heterotrophic bacteria and hydrocarbon-utilizing bacteria isolated from the polluted and pristine rhizosphere and non-rhizosphere soils of the plants were compared. The polluted rhizosphere of total culturable heterotrophic bacterial count gave a range of 0.98x10 6 cfu/g to 1.37x10 6 cfu/g. The pristine rhizosphere count ranged from 4.11x10 5 cfu/g to 7.55x10 5 cfu/g. The polluted non-rhizosphere gave ranged from 2.39x10 5 cfu/g to 3.28x10 5 cfu/g. The pristine non-rhizosphere had a range of 2.90x10 5 cfu/g to 3.97x10 5 cfu/g. The polluted rhizosphere counts for hydrocarbon-utilizing bacteria ranged from 1.60x10 5 cfu/g to 6.91x10 5 cfu/g. The pristine rhizosphere gave a range of 1.85x10 5 cfu/g to 3.38x10 5 cfu/g. In the polluted non-rhizosphere, the range was from 1.02x10 5 cfu/g to 1.42x10 5 cfu/g. A range of 6.05x10 4 cfu/g to 9.75x10 4 cfu/g was obtained from the pristine non-rhizosphere. There was no significant difference (P>0.05) between the rhizosphere and non-rhizosphere of total heterotrophic and hydrocarbon-utilizing bacterial counts in both polluted and pristine soils. All the plants exhibited positive rhizosphere effects on the rhizobacteria. Hydrocarbon-utilizers were identified as Acinetobacter,
The Rhizosphere Effect on the Bacterial Genera Associated with Crude Oil Polluted Soil Ecosystem
The rhizo-bacterial genera associated with these plants Cyperus amabalis, Desmodium triflorum, Phaseolus sp., Solenstemon sp., Mariscus sp were isolated, enumerated and studied. Statistical analysis showed no significant difference (P>0.05) between the rhizosphere and non-rhizosphere of total culturable heterotrophic and hydrocarbon-utilizing bacterial counts in both polluted and unpolluted soils. The rhizosphere effect values of the above named plants were determined. All the plants exhibited positive rhizosphere effects on the rhizo-bacteria. The rhizosphere effect ratio of the hydrocarbon-utilizing bacteria showed significant difference between the polluted and unpolluted soils of Phaseolus sp. (P=0.022), Solenstemon sp. (P=0.012). The hydrocarbon utilizing bacteria isolated were identified as Acinetobacter, Alcaligenes, Arthrobacter, Corynebacterium, Flavobacterium, Pseudomonas and Serratia spp. All the isolates grew on petroleum hydrocarbon at different growth rates. Based on these results, the hydrocarbon utilisers isolated can serve as seeds for bioaugmentation during remediation of crude oil polluted soil while the plants may be employed in rhizoremediation of oil polluted soil. Keywords: Rhizosphere effect, Bacterial genera, Oil spill, Soil ecosystem, Bioremediation, Hydrocarbons.
Bulletin of environmental contamination and toxicology, 2015
Different grasses and trees were tested for their growth in a crude oil contaminated soil. Three grasses, Lolium perenne, Leptochloa fusca, Brachiaria mutica, and two trees, Lecucaena leucocephala and Acacia ampliceps, were selected to investigate the diversity of hydrocarbon-degrading rhizospheric and endophytic bacteria. We found a higher number of hydrocarbon degrading bacteria associated with grasses than trees and that the endophytic bacteria were taxonomically different from rhizosphere associated bacteria showing their spatial distribution with reference to plant compartment as well as genotype. The rhizospheric soil yielded 22 (59.45 %), root interior yielded 9 (24.32 %) and shoot interior yielded 6 (16.21 %) hydrocarbon-degrading bacteria. These bacteria possessed genes encoding alkane hydroxylase and showed multiple plant growth-promoting activities. Bacillus (48.64 %) and Acinetobacter (18.91 %) were dominant genera found in this study. At 2 % crude oil concentration, all...
2022
The study investigated microbial population dynamics in soils of Greater Port Harcourt Area, Nigeria. The study involved the quantification of petroleum pollutants and the enumeration and characterization of culturable fungi and bacteria. Gas Chromatograph FID method was used to quantify total petroleum hydrocarbons (TPH) in the soil while morphological and biochemical tests were used to characterize the microorganisms. The highest TPH was 9,759.37±883.36 ppm which exceeded the Department of Petroleum Resources' (DPRs') intervention value of 5,000 ppm. The percentage of hydrocarbon utilizing fungi (% HUF) ranged between 1.93±0.57 and 66.55±5.60 which were observed in sites A1 (Aluu) and CA (Agricultural control), respectively. The percentage of hydrocarbon utilizing bacteria (% HUB) ranged between 0.12±0.01 and 7.89±1.25 which was observed in sites A3 (Emuoha) and CU (Urban control), respectively. The most prevalent bacterial species includes Bacillus subtilis, Micrococcus lylae, Staphylococcus aureus, Bacillus cereus and Alcaligen faecalis. The most prevalent HUB isolate was Bacillus subtilis. The most prevalent fungal isolates were Aspergillus niger, Aspergillus flavus and Candida torulopsis. A. niger was the most prevalent among the HUF isolates which was identified in 12 sites. It was concluded that the study sites harboured TPH degrading microorganisms that are appropriate for bioremediation of TPH polluted sites.
International Journal of Microbiology, 2020
The Sudd wetland is one of the oil-rich regions of South Sudan where environmental pollution resulting from oil extraction activities has been unprecedented. Although phytoremediation is the most feasible technique, its efficacy reduces at high TPH concentration in soil. This has made rhizoremediation the most preferred approach. Rhizoremediation involves use of a combination of phytoremediation and biostimulation. The process is catalyzed by the action of rhizobacteria. Therefore, the objective of this study is to characterize rhizobacteria communities prevalent in phytoremediation species growing in hydrocarbon-contaminated soils biostimulated with cattle manure. The treatments studied were plant species only (T1), plant species and hydrocarbons (T2), plant species and manure (T3), and plant species, manure, and hydrocarbons (T4). The rhizobacteria communities were determined using pyrosequencing of 16S rRNA. In the treatment with phytoremediation species, hydrocarbons 75 g · kg−1...