Moroccan Journal of Chemistry Investigation of antibacterial & antioxidant activity for methanolic extract from different edible plant species in Palestine (original) (raw)
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Journal of Biological Sciences, 2010
Antibiotic resistance has been called one of the world's most pressing public health problems. Irrational use of antibiotics promotes development of resistance against bacteria. The antimicrobial properties of medicinal plants are being increasingly reported from different parts of the world as they found to have lesser side effects and devoid of drug resistance. In an effort to expand the spectrum of antibacterial agents from natural sources the present study aimed to evaluate antibacterial and antioxidant activity of essential oil from the flowers of Michelia champaka (M. champaka). Essential oil was extracted from flowers of M. champaka by hydro distillation method using Clevenger's apparatus and screened for its antibacterial activity against gram positive (Micrococcus luteus, Streptococcus mutans) and gram negative (Escherichia coli, Salmonella typhi) strains. The minimum Inhibitory Concentration (MIC) of oil was determined using an agar diffusion method. Nevertheless the peroxide antioxidant assay was also performed. It was observed that essential oil has shown remarkable antibacterial activity against Streptococcus mutans (the causative agent for dental caries) with a zone of inhibition of 30 mm. A zone of inhibition was compared with standard antibiotic gentamicin. The MIC was reported as 25 mg/ml. The % inhibition of peroxide free radical was found to be 63.7% at 200mg/ml concentration. The antibacterial and antioxidant activity of M. champaka essential oil (MCEO) was due to various phytoconstituents. Hence MCEO can be used as an antibacterial source and phytoconstituents responsible for this activity can be explored and utilized in discovery of leads in the area of pharmacy research.
research journal of pharmacy and technology, 2022
In this study, the methanolic extracts from four medicinal plants (Sarcopoterium spinosum, Paronychia argentea, Inula viscosa, and Achillea fragrantissima) were tested in vitro for antibacterial and antioxidant properties. The antibacterial properties of these MPEs were assessed using disc diffusion and Serial dilution techniques. The antioxidant properties were evaluated by DPPH and FRAP methods. The total phenolic compounds were measured according to Folin-Ciocalteu method. Differences were found among the tested plants in their antibacterial and antioxidant properties as well as the phenolic content. Among the four plants, I. viscosa revealed the highest antibacterial effects against all bacterial species under examination followed by S. spinosum. Gram-positive bacteria showed higher susceptibility to the MPEs than Gram-negative bacteria. Antioxidant capacity and polyphenolic content investigations revealed that S. spinosum and I. viscosa have the highest antioxidant activities and the highest amount of phenolic compounds. Moreover, a strong positive linear correlation was found between antioxidant activity and total phenolic content. In conclusion, the MPEs of I. viscosa and S. spinosum have been proven to have good antibacterial and antioxidant activities which were highly related to their contents of phenolic compounds. These plant extracts could be interesting sources for natural antibacterial and antioxidant agents.
European Journal of Medicinal Plants, 2015
To evaluate eleven medicinal plants as natural sources that possess strong antidermatophytic, antibacterial, anticandidal and antioxidant substances with potential applications in therapeutics and food industry. Place and Duration Methodology: Twenty methanolic extracts were prepared from different parts of eleven plants used in traditional medicine in Palestine. The plants extracts were screened for total flavonoid and phenolic content using standard procedures. The crude extract was screened against six bacterial strains (Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, and Klebsiella pneumoniae), 5 Candida albicans strains, and 2 dermatophytes EJMP, 9(1): 1-13, 2015; Article no.EJMP.17429 2 (Microsporum canis, and Trichophyton rubrum). The antioxidant potential of the crude extract was also determined using the DPPH assay. Results: The best free-radical scavenging was for the leaves of Epilobium hirsutum (IC 50 =33 µg/ml) and Rhus coriaria (49 µg/ml) compared with BHA standard (9 µg/ml). The highest value of phenolics was in R. coriaria fruits (14.7 mg/g dried plant material) and for flavonoids was for Epi. hirsutum leaves (1.14 mg/g). The most active extracts against bacteria was the R. coriaria leaves (% inhibition, 66.2%) compared with gentamicin (100%) and against Candida were leaves of R. coriaria (100%) and Epi. hirsutum (72.4%) compared with amphotericin B (100%). On the other hand fruits of R. coriaria showed the best antifungal activity against all the tested dermatophytes, 97% and 86% inhibition were achieved against Microsporum canis and Trichophyton rubrum, respectively. Conclusion: Our results introduce a natural source (R. coriaria and Epi. hirsutum) that possesses strong antidermatophytic, antibacterial, anticandidal and antioxidant substances with potential applications in therapeutics and food industry.
BMC Chemistry
Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.
The antioxidant and antibacterial properties of methanolic extracts derived from aerial parts of three libyan native plants including Origanum majorana L. Helichrysum stoechas and Polygonum aviculare L. were assessed. Variations were observed among the examined plants in antioxidant and antibacterial activities as well as in their Total phenol content (TPC) and Total flavonoid content (TFC). TPC and TFC ranged from 3.07 to 8.07mg gallic acid equivalents/g on a dry basis and from 0.56 to 2.04mg catechin equivalent/g on a dry basis respectively. Helichrysum stoechas contained the highest amount of phenolic and flavonoid compounds followed by Polygonum aviculare L. and Origanum majorana L. respectively. Antioxidant capacity using DPPH, ABTS and reducing power were measured. Helichrysum stoechas had the highest antioxidant capacity compared to the other tested plants. Minimum inhibitory concentrations (MIC) and minimum lethal concentrations (MLC) were determined to verify the antimicrobial activities of the plants against 12 food-borne pathogens and food spoilage bacteria. All investigated plants had potent antimicrobial activity against tested bacteria. Bacillus cereus ATCC 10876, was found to be the most susceptible bacteria. Phytochemical screening of the extracts revealed that all plants contained saponin, tannins whereas alkaloids were excluded to Helichrysum stoechas and Origanum majorana L. Consequently, these results may suggest that Helichrysum stoechas, Polygonum aviculare L. and Origanum majorana L. could be used as antibacterial and antioxidant agent suggesting their potential application as natural preservatives in foods.
The present study describes the anti-bacterial activity of methanol extract of OpuntiastrictaF.,TrachyspermumammiS.,Terminaliachebula F.and T.citrinaF. against bacteria include Staphylococcus aureus(PTCC 1431), Bacillus cereus (PTCC 1015), Escherichia coli (PTCC 1338),and Klebsiellapneumonia (PTCC 1290). For this purpose extraction weredone by macerationmethod and tested by agar welldiffusion method. As a result of this study it was found that the all of extracts generally revealed anti-bacterial activity against both gram-positive bacteria and gram-negative bacteria. Staphylococcus aureus was the most active bacteria. The MIC value of 4 plant extract Opuntia strictaF.,TrachyspermumammiS., Terminaliachebula F.and T. citrinaF. against Staphylococcus aureus was 1.25,2.5,2.5 and 10 mg/ml respectively.
Journal of Advanced Biotechnology and Experimental Therapeutics, 2019
ABSTRACT: The aim of this study was to explore phytochemical profiling, antioxidant and antibacterial activity of four medicinal plants including Catharanthus roseus, Aegle marmelos, Moringa oleifera, and Ageratum conyzoids grown in Sylhet district, Bangladesh. In this study, total 11 phytochemicals were screened from methanol extract of four medicinal plants, wherein flavonoid, tannin, sterol, phenol were present in all four medicinal plants. In vitro, antioxidant activity of these medicinal plants extract was investigated by DPPH-radical scavenging assay. The Aegle marmelos exhibited the highest antioxidant activity followed by Moringa oleifera, Ageratum conyzoids, and Catharanthus roseus extract. Methanolic extracts of same medicinal plants were subjected to a test of their antibacterial activities against Staphylococcus aureus, Escherichia coli, Klebsiella sp., Pseudomonas sp .and Salmonella sp. by agar disc diffusion method. The highest antibacterial potential was observed in the extract of Aegle marmelos against Salmonella sp. followed by Catharanthus roseus against Pseudomonas sp .with zone of inhibition of 18.67 mm, 15.0 mm, respectively. This study confirmed the efficacy of some native medicinal plants extract as potential source of phytochemicals, along with natural antioxidant and antimicrobials, which provide new possibilities to employing them against disease causing test organisms.
Screening of the antimicrobial activity of some extracts of edible wild plants in Morocco
Functional Foods in Health and Disease
Background: Despite the availability of cultivated food crops and processed food, a large part of the Moroccan population, more particularly the populations of rural areas, still depend on the traditional use of wild plants, which constitute an important component of their food system. However, there is a lack of information on these plants and their medicinal and pharmacological properties, this is why our study aims to detect the antimicrobial activity of certain wild edible plants.Methods: disc diffusion method was used to evaluate the antimicrobial activities of extracts of Mercurialis annua L, Papaver rhoeas L, Foeniculum vulgare Mill, Chenopodium mural L, and Scolymus hispanicus L against the bacterial species Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonassp, and against the yeasts Cryptococcus neoformans, and Candida albicansResults: The results showed that the crudeextracts from all the plants studied showed more or less important antimicrobial a...