Comparative Analyses of the Coat Protein of Newly Discovered Ampelovirus Using Computer-Assisted Phylogenetic Reconstruction and Analysis: (An Approach for Classification and Characterization) (original) (raw)
The coat protein of the newly found closteroviruses Grapevine leafroll associated vir rus 9 (GLRaV-9) was used for comparative analysis with other members of the family Closteroviridae. The amino acid alignments using ClustalW demonstrated a significant homology between the GLRaV-9 sequence and the Grapevine leafroll associated virus 5 (86%) and Pineapple mealybug wiltrassociated virusr1 (56%). GLRaV-9 and GLRaV5 were proved to be serologically distinct by using GCG sequence analysis software package that indicate the significant variation (26%) within their polypeptide sequences at the N-terminus of their coat protein. The phylogenetic analysis, grouped GLRaV-9 with mealybug transmitted viruses (GLRaV-1, -3, -5, and PMWaV-1, -2), suggesting the mode of the transmission of this newly found closterovirus. * * Corresponding author: ralkowni@aauj.edu :صخللما في همادختس�ا تّم دق )Grapevine leafroll associated virus 9( ىمس�لماو اثيدح فس�تكلما س�ويرفلل فلغّلما ينتوبرلا مادختس�اب ةينيملاا ...
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