Investigating hsp gene expression in liver of Channa striatus under heat stress for understanding the upper thermal acclimation (original) (raw)
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Biochemical and Biophysical Research Communications, 2005
The genes encoding heat shock cognate 70 (hsc70) and inducible heat shock protein 70 (hsp70) were cloned and characterized from silver sea bream liver. Upon acute heat shock (+7°C), the transcript abundance of hsc70 was increased 1.7-fold whereas the transcript abundance of hsp70 increased 6.7-fold. The chronic acclimation of sea bream to cold temperature (12°C) resulted in a downregulation of hsc70 and an upregulation of hsp70 in comparison to levels in sea bream kept at a warmer temperature (25°C). The expression of heat shock transcription factor I was also increased during cold temperature acclimation. Increased amounts of hepatic insulin-like growth factor 1 transcript, serum thyroxine (T 4), and triiodothyronine (T 3) were also found during cold temperature acclimation whereas serum cortisol remained unchanged. The results from this study demonstrate how temperature acclimation, in fish, can affect the regulation of the hsp70 multigene family and hormonal factors that are associated with anabolism.
Proteomic changes in the liver of Channa striatus in response to high temperature stress
Electrophoresis, 2016
The present study was undertaken to investigate the proteomic changes in liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36°C for 4 days and liver proteome changes were analyzed using gel- based proteomics i.e. 2D gel electrophoresis, MALDI-TOF/TOF-MS and validation by transcript analysis. The study showed, besides others, increased abundance of two sets of proteins, the antioxidative enzymes SOD, ferritin, CRBP, GST and the chaperones HSP60, PDI; this was validated by transcript analysis. The proteome data are available via ProteomeXchange with identifier PXD002608. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer durations (30 days experimental exposure in laboratory and for 30 days beyond, taking Channa collected from a hot spring runoff at 36-38°C); sod, gst, crbp and hsp60 were found to continue to remain up regulated at 8, 2.5, 2.4 and 2.45 folds, respectively in the hot spring ...
Expression of heat shock protein 70 in Cirrhinus mrigala (Ham.) exposed to thermal variations
2004
The present investigation reveals the results of heat and cold shock expression of HSP-70 in different organs of Cirrhinus mrigala (Ham.). Fishes were exposed to heat and cold shock treatments and thermosensitive organs viz. liver, brain, kidney and gills were dissected out and tissues were analyzed for HSP70 quantification. At acclimatised temperature (260 C) the HSP – 70 level was highest in liver (10.35 ± 0.8704) and lowest in gills (1.96 ± 0.29). The highest HSP-70 levels were obtained in brain and liver, respectively during heat shock treatment at 370 C for 1 hr, 2hrs, 4 hrs, 22 hrs, 27 hrs, 44 hrs and 48 hrs whereas the lowest HSP-70 levels were obtained in kidney and gills, respectively. In cold shock treatment at 170 C for 18 hrs, 27 hrs and 48 hrs the highest HSP-70 levels were obtained in liver and brain, whereas the lowest levels were obtained in gills.
Biological Chemistry, 2000
The native cold-adapted brown trout (Salmo trutta fario) is often the subject of biomonitoring field studies. Groups of trout were exposed to a sudden temperature rise, from 8°C to 19°C for two hours, and thereafter set back to 8°C. Gill samples of control animals, of fish after the exposure period, and after 24 and 48 hours of recovery at a temperature of 8°C were examined histologically, immunohistochemically, electron microscopically, and by Western blot analysis. By means of immunohistochemistry and electron microscopy, an increase of chloride cells was observed after the temperature elevation. During the recovery period the number of chloride cells decreased. Western blot analysis for stress proteins (hsp70), widely used as a biomarker for environmental stress, was performed from skin and gill. Whereas in the gill both isoforms, the constitutive and the heat inducible form, of hsp70 were detected in all groups, in the skin the control animals only showed the constitutive form. After two hours of exposure both isoforms were visible. An increased expression of hsp70 could be demonstrated in both organs after the exposure. Comparison of the hsp70 values between gill and skin showed tissue-specific differences during the recovery period. In the gill hsp70 rapidly decreased, while in the skin the level remained elevated over the whole observation period. When hsp70 is used as a biomarker in field studies, the fast and organ-specific reaction in the gill and skin of brown trout has to be taken into consideration.
2019
An increase in temperature can be a significant stressor for aquatic organisms. Abalones, a type of single-shelled gastropod, are the most important commercial species in shellfish cultivation in Indonesia. To evaluate the potential ecological risks posed by temperature stress, we measured biological responses such as survival rates, mucus production, muscle hardness and histologically foot muscle abnormalities in Haliotis squamata. In addition, biochemical responses and gene expression profile were also evaluated in the exposed experiment to various temperature gradients. Thermal shock was carried out in 4 rectangular glasses aquarium (100 L). Abalones were divided into four groups for temperature treatment, in triplicates. The temperature treatments ware adjusted to 28, 30, 32 and 34C in advance. Every aquarium contained 3 pcs of 20 cm long 3” PVC pipe for rearing 30 abalones 32.97±1.83 mm per PVC. Following 4 days of temperature exposure, the dead abalones were collected from ea...
Heat shock protein expression in fish
Reviews in Fish Biology …, 1998
page 35 General background 36 Molecular processes related to HSP expression 37 Heat shock protein studies in ®sh 40 Cell line studies Primary cell culture Whole animal studies Molecular characteristics of ®sh heat shock proteins The generalized stress response and HSP expression Methods of analysis 48 Applications 49 Acknowledgements 51 References 51
Cell Stress and Chaperones, 2011
The heat shock proteins (HSPs) are a family of proteins whose expression is enhanced in response to environmental stressors. The Apostichopus japonicus hsp90 and hsp26 genes were cloned using expressed sequence tag and rapid amplification of cDNA ends techniques. The full-length cDNA of Aphsp90 and Aphsp26 contains 3,458 and 1,688 nucleotides encoding 720 and 236 amino acids, respectively. Multiple alignments indicated that the deduced amino acid sequences of ApHsp90 and ApHsp26 shared a high level of identity with Hsp90 and small SHPs (sHSPs) sequences of zebrafish, ant, acorn worms, etc., and shared identical structural features with Hsp90 and sHSPs. The expression profiles of these two genes under heat treatment were investigated by real-time quantitative PCR. It was found that the messenger RNA (mRNA) transcripts of the two A. japonicus genes varied among different tissues under normal conditions and heat shock, and that the mRNA expression of the two genes was higher in the intestine compared to other tissues. Heat shock significantly elevated the expression of Aphsp90 and Aphsp26 mRNA in a temperature-and time-dependent manner. The results indicate that Aphsp90 and Aphsp26 played important roles in mediating the environmental stress in A. japonicus.