Properties of IncP-2 plasmids of Pseudomonas spp (original) (raw)
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Resistance Plasmids of Indigenous Pseudomonas in Egypt
Journal of Applied Sciences Research, 2007
Two hundred forty nine Pseudomonas isolates were collected from different geographical sites in Egypt. They were examined for resistance against ten heavy metals and five antibiotics. Plasmid profiles were also studied. Eight P. aeruginosa and two P. fluorescence strains were selected for further studies. They were resistant to seven to nine heavy metals and four to five antibiotics. The ten strains contain two small plasmids, but have up to five large plasmids. Plasmid curing indicated that resistant to each of cadmium, cobalt, silver, chromium, mercury, nickel or zinc is plasmid borne. It also showed that the resistance of both iron and lead are carried by the bacterial chromosome. Results also revealed that chromate resistance gene (s) were located on a large plasmid in P. aeruginosa24 strain. The resistance of all tested antibiotic was located in plasmids.
Antibiotic sensitivity and plasmid profiles of Pseudomonas aeruginosa
The Central African journal of medicine, 2000
Background: The emergence of drug resistance among diarrheagenic Escherichia coli (E. coli) in the pediatric population is an important cause of morbidity and mortality in developing countries. Material and Methods: Isolation and identification of E. coli strains from stool specimens are carried out according to standard techniques. Antibiotic susceptibility testing was performed using disc-diffusion method. Plasmid profiling and conjugation experiments were done to analyze the antibiotic resistance transfer from one bacterium cell to another through plasmid. Results: Out of 170 pediatric diarrheal samples, 105 (61.76%) E. coli strains were isolated. About 90% of E. coli strains were resistant to most of the antimicrobial agents tested. All the isolates were resistant to ampicillin, imipenem and cotrimoxazole and were sensitive to amikacin. The resistance to antibiotics shows 29 different antibiotic resistance patterns. About 67 (64%) strains of E. coli isolates harbored plasmids, and 51 (76.1%) of them were able to transfer their plasmids. The plasmid sizes ranged from 1.0 to 25 kb, the most common plasmid of size 4.8 kb being detected in all the plasmid-harbored E. coli strains. The results of transconjugation show that all the transconjugant colonies were carrying 4.8-kb plasmid and were resistant to ampicillin, imipenem and cotrimoxazole. Conclusion: There is an increase in the prevalence of drug resistance among E. coli isolates, and conjugal transfer of plasmids has greatly contributed to the rapid spread of antibiotic resistance among E. coli isolates.
Inheritance of IncP-9 Catabolic Plasmids in Pseudomonas Bacteria
Russian Journal of Genetics, 2019
This work investigated the capability of the conjugative transfer of epsilon-caprolactam biodegradation (CAP) plasmids, belonging to incompatibility (Inc) groups P-2, P-7, P-9, and IncP-9 naphthalene biodegradation (NAH) plasmids to various species of Pseudomonas bacteria, including strains of plant growth-promoting rhizobacteria (PGPR), and the character of plasmid inheritance in these strains. The frequency of the conjugative transfer of all CAP and NAH plasmids (irrespective of their Inc groups) into Pseudomonas PGPR was shown to be lower than that into P. putida soil strains by more than an order of magnitude. The low frequency of conjugative transfer was found not to be caused by secretion of antibiotically active metabolites by Pseudomonas PGPR strains. Stability of inheritance of catabolic plasmids under nonselective conditions was established to depend on their Inc group and bacterial host strain. Thus, all investigated plasmids were stably maintained in P. putida KT2442 and BS394 strains for at least 100 generations. However, only CAP plasmids of IncP-2 and IncP-7 groups were stably maintained in Pseudomonas PGPR cells. CAP and NAH plasmids of different IncP-9 subgroups were eliminated from cells of rhizosphere pseudomonads within several generations. Thus, the work first revealed inheritance features of IncP-9 catabolic plasmids in Pseudomonas PGPR cells.
Isolation of a 29.5 kb Plasmid conferring Multiple Drug Resistance in Pseudomonas aeruginosa
Journal of Bio-Science, 2011
Context: Worldwide emergence of plasmid mediated multi drug resistant bacterial strain is a growing concern, especially in hospital infections caused by Pseudomonas aeruginosa. Relation of plasmid and drug resistance in clinical isolates of P. aeruginosa by curing and transformation experiments is scanty.Objectives: To isolate, purify and characterize plasmid DNA harbored in a selected Pseudomonas aeruginosa strain encoding multiple drug resistance and to perform transformation of the isolated plasmid into a sensitive strain of Escherichia coli LE 392 to judge transformation potential of the donor P. aeruginosa strain. Materials and Methods: Plasmid DNA was isolated from a multidrug-resistant (MDR) strain of P. aeruginosa obtained from swab of a hospitalized burn patient by mini-scale method. DNA was purified, quantitatively estimated and electrophoresed on 0.8% agarose gel. Transformation was done as per Cohen and co-workers using plasmid DNA isolated from MDR P. aeruginosa strain ...
Diversity of IncP-9 Plasmids of Pseudomonas
…, 2008
IncP-9 plasmids are important vehicles for degradation and resistance genes that contribute to the adaptability of Pseudomonas species in a variety of natural habitats. The three completely sequenced IncP-9 plasmids, pWW0, pDTG1 and NAH7, show extensive homology in replication, partitioning and transfer loci (an~25 kb region) and to a lesser extent in the remaining backbone segments. We used PCR, DNA sequencing, hybridization and phylogenetic analyses to investigate the genetic diversity of 30 IncP-9 plasmids as well as the possibility of recombination between plasmids belonging to this family. Phylogenetic analysis of rep and oriV sequences revealed nine plasmid subgroups with 7-35 % divergence between them. Only one phenotypic character was normally associated with each subgroup, except for the IncP-9b cluster, which included naphthalene-and toluene-degradation plasmids. The PCR and hybridization analysis using pWW0-and pDTG1-specific primers and probes targeting selected backbone loci showed that members of different IncP-9 subgroups have considerable similarity in their overall organization, supporting the existence of a conserved ancestral IncP-9 sequence. The results suggested that some IncP-9 plasmids are the product of recombination between plasmids of different IncP-9 subgroups but demonstrated clearly that insertion of degradative transposons has occurred on multiple occasions, indicating that association of this phenotype with these plasmids is not simply the result of divergent evolution from a single successful ancestral degradative plasmid.
Canadian Journal of Microbiology, 1994
1994. Relative fitness in vitro and in planta of P.se~rc1orizotin.s .s)~ringne strains containing copper and streptomycin resistance plasmids. Can. J. Microbiol. 40: 279-285. The effect of resistance plasmids encoding copper resistance (Cur), streptomycin resistance (Smr), and both Cur and Smr on competitive fitness of P.se~rclottzotinss)~ringne pv. s)~rit~gcie was studied in vitro and in planta. The Cur Smr plasmidpPSR I provided a selective advantage to its bacterial host (P.se~~dot~zotzns s)lritzgae pv. syrit~gae FF5.1) on Pvr~1.s cnlletynrla leaves that were treated weekly with copper and (or) streptomycin bactericides. However, populations of the plasmid-free CuS Sm" FF5.1 were reduced 10-to 1000-fold over a 12-week period on trees treated with bactericides. The resistance plasmids pPSR4 (Cur), pPSR5 (Smr), and pPSR4::Tn5393 (Cur Smr) were highly stable for over 200 generations of growth in glucose-limited batch culture. Results of competition experiments in vitro indicated that P.se~~cloti~otzn.s syringae pv. .syritzgne FF5 containing pPSR4, pPSR5, or pPSR4::Tn5393 was reduced to less than 5% of the total culture in competition with wild-type FF5. In growth chamber studies, the resistance plasmids studied did not have an impact on epiphytic fitness of P,se~donzotins s)vitigne pv. syrirzgne. Our data suggest that resistance plasmids will persist in populations of P.ve~doniotin.s syritzg~le pv. syritzgne following their initial selection regardless of the bactericidal spray regime.
The present study has concentrated on the antibiotic resistance pattern and transferability of the plasmid mediated resistance of marine bacteria. A total of 9 different samples comprising of rhizosphere of coastal sand dune plants and sea water were used in the study to isolate fluorescent pseudomonads (FPs). From these samples pseudomonas were isolated by spread plate technique using Kings B agar about 60 FPs were isolated and were screened primarily for pigment production in KB agar. When screened for antibiotic resistance the isolates AMET6109 and AMET6152 have exhibited average growth in the presence of high concentration of chloramphenicol .For plasmid transfer experiments a total of nine donor strains (Pseudomonas sp) were tested for three different donor recipient (Escherichia coli JM109) ratio. The co cultured bacteria were spread plated on EMB agar amended with Chloramphenicol (100 μg/mL concentration). To demonstrate the transformed chloramphenicol resistant plasmid from E.coli, the transformed E.coli was streaked on antibiotic amended EMB agar plate. The ability of transformed bacterium in subsequent generations in the antibiotic amended medium denotes the survival of the transferred plasmid.
European Journal of Epidemiology, 1992
Antibiotic resistance phenotypes, plasmid content and ability of conjugal transfer of antibiotic resistance genes of 35 multi-resistant Pseudomonas aeruginosa strains were examined. The strains were isolated in 12 Greek hospitals and the majority of them (80%) belonged to serotype 0:12. The isolates were distributed to a variety of different antibiotic resistance phenotypes. Plasmid analysis showed that 10 isolates harboured plasmids ranging in size from 20 to 100 Mda. Among these strains, four carried plasmids of 100 Mda, two strains had 60 Mda plasmid each while in three strains the plasmids detected were 65, 25 and 20 Mda, respectively. One strain harboured two plasmids of 100 and 60 Mda. All strains containing plasmids belonged to 0:12 serotype, except the one harbouring the 25 Mda plasmid, which belonged to serotype 0:6. Using a P. aeruginosa recipient resistant to rifampicin and ciprofloxacin, conjugal transfer was achieved in two occasions. These plasmids, 100 Mda in size, encoded high-level resistance to both gentamicin and tobramycin whereas resistance to other drugs was not transferable. Interestingly, all 100 Mda plasmids, including the self-tansferable ones, were found to share a certain degree of homology as judged by restriction analysis. It is suggested that both resistance phenotypes and analysis of plasmid content might be useful in subdividing 0:12 multi-resistant P. aeruginosa.