Inhibition of pancreatic lipase by gallic acid and quercetin equivalent in ultrasonicated Malaysian grown Aquilaria spp. leaves of different particle size (original) (raw)

Article Info Pancreatic lipase inhibitory compounds (gallic acid and quercetin) and their inhibition properties were determined in the crude extract of matured leaves from widely grown Aquilaria spp. in Malaysia, which are Aquilaria malaccensis and Aquilaria subintegra. The leaves were dried at 60 °C for 24 hours and undergone a pre-treatment method by using ultrasonication, within 30 minutes of reaction. Dried matured leaves were ground and sieved into particle sizes of 250, 300, 400, 500, and 1000 μm. Each particle size was soaked in distilled water with a ratio of 1% (w/v) for 24 hours, prior to ultrasonication process. Particle size of 250 μm resulted in the highest concentration of gallic acid and quercetin equivalent for both species of Aquilaria, wherein 89.99 mg/ml of gallic acid equivalent and 0.0295 mg/ml of quercetin equivalent were obtained in A. malaccensis crude leaves extract and in A. subintegra crude extract, 101.27 mg/ml and 0.0373 mg/ml were obtained, respectively...

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