Protective effect of antioxidant rich aqueous curry leaf (Murraya koenigii) extract against gastro-toxic effects of piroxicam in male Wistar rats (original) (raw)
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Nutrition Research, 2003
Curry leaf (Murraya koenigii) is commonly used in India as a natural flavoring agent for various food preparations. Drinking water containing 10% and 20% fresh crude curry leaf aqueous extract was given to Swiss albino mice to investigate its ability to modulate the level/activities of drug metabolising Phase I enzymes (Cytochrome P450, Cytochrome b5, Cytochrome P450 reductase and Cytochrome b5 reductase), Phase II enzymes (Glutathione S-transferase and DT-diaphorase), antioxidant parameters (Reduced glutathione, Superoxide dismutase, Catalase, Glutathione peroxidase, Glutathione reductase), lactate dehydrogenase and lipid peroxidation changes. The level of glutathione, glutathione S-transferase and DT-diaphorase was examined also in the extrahepatic organs viz., forestomach, kidney and lung. There was a significant increase in the activities of acid soluble sulfhydryl (-SH) content (from P Ͻ 0.05 to P Ͻ 0.001), glutathione-S transferase and DT-diaphorase (from P Ͻ 0.005 to P Ͻ 0.001). Antioxidative parameters were also elevated above basal value being: superoxide dismutase (P Ͻ 0.005), catalase (P Ͻ 0.001), Glutathione peroxidase (P Ͻ 0.005), and glutathione reductase (P Ͻ 0.005). Level of lipid peroxidation and lactate dehydrogenase decreased significantly. Anticarcinogenic potential of curry leaf was evaluated adopting the protocol of Benzo-(a)pyrene induced forestomach and 7,12 Dimethylbenz(a)anthracene (DMBA) induced skin papillomagenesis. Chemopreventive response was measured by tumor burden (papillomas/mouse), and by the percentage of tumor bearing animals. Both the investigated dose levels of curry leaf showed a significant reduction in tumor burden (P Ͻ 0.001) as well as tumor incidence at both the tumor model system studied. Our studies strongly suggest that the curry leaf can be useful for the prevention of human stomach and skin cancers.
Khaya senegalensis inhibits piroxicam mediated gastro-toxicity in wistar rats
Avicenna Journal of Phytomedicine, 2014
Objective: The purpose of this study was to investigate the effects of piroxicam co-administration with ethanolic stem-bark extract of Khaya senegalensis on biomarkers of oxidative stress and gastro-toxicity in Wistar rats. Materials and Methods: Thirty healthy male and female Albino Wistar rats (190-220 g) were grouped into six (n = 5) with designated treatments including: Normal saline, piroxicam (20 mg/kg), extract (200 and 400 mg/kg) alone and both doses of the extract co-administered with piroxicam. The drugs were administered orally to all the rats for fourteen consecutive days and on the fifteenth day, they were euthanized with chloroform inhalation. Blood samples and the stomachs were isolated for evaluation of the oxidative stress biomarkers and gastro integrity, respectively. Results: The results of the study revealed that the levels of oxidative stress markers didn’t differ significantly between the groups receiving the extract alone, the extract in combination or piroxic...
Phytochemical profiling of curry (Murraya koenijii) leaves and its health benefits
2015
Nutraceuticals and functional foods provide a chance to attenuate different physiological threats by improving the human health. Powerful bioactive components are responsible for antioxidant properties of herbs that help to alleviate different metabolic disorders. Plant based products are defensive against various metabolic disorders because of their bioactive components. Murraya Koenijii is a spicy plant that contains significant amount of vitamin C and minerals viz., calcium, iron, potassium and sodium. Major phyto-chemicals present in curry leaves are phenolic, carbazole alkaloids and phenols. Bioactive components in Murraya Koenijii can be extracted by different methods i.e. solvent extraction, soxhlet extraction and hydro-distillation. Antioxidant property of curry leaves helps to protect from high cholesterol level, cardiovascular diseases, diabetes and many other degenerative illnesses. In bakery products, replacement of wheat flour with curry leaf powder increase the caroten...
The present study was carried out for determination of antibacterial activity and phytochemical screening of Curry (Murraya Koenigi) leaves solvent extracts. Four solvents were used for extraction. These are Ethanol, Methanol, Diethyl Ether and Acetone. The solvent extraction was done by using Soxlet apparatus. Test microorganisms were screened to confirm their viability and identities using standard microbiological methods. The different solvent extracts of Curry leaves was tested for antimicrobial activity using the standard agar well diffusion method against nine enteric pathogens, these are E.coli, Salmonella typhi, Sal.para.A, Sal. para. B, Shigella sonnei, Shigella dysentarie, Enterobactor spp.,Citrobactor spp.and Klebsiella spp. The Ethanol extract of Curry leaves showed highest antimicrobial activity against Shigella sonnei. The Methanol extract of Curry leaves showed highest antimicrobial activity against E.coli. The Diethyl ether extract of Curry leaves showed highest antimicrobial activity against Sal.para.A while Acetone extract showed highest antimicrobial activity against Sal.typhi. The antimicrobial activity of standard antibiotics Ampicillin and Tetracycline were studied in comparison with Curry leaves solvent extracts. The MIC values were determined by both agar and broth dilution method. The functional chemical group was determined by Fourier Transform Infrared Spectroscopy (FTIR).The phytochemical analysis of Curry leaves solvent extracts showed presence of Alkaloid, Flavonoid, Phytosterol, Saponin, Glycosides, Phenolic compounds, Terpenoid etc.