Antidiabetic Activity and Phytochemical Screening of Crude Extract of Stevia Rebaudiana in Alloxan-induced Diabetiis Rats (original) (raw)
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BMC complementary and alternative medicine, 2018
Stevia (Stevia rebaudiana) natural, non-caloric sugar substitute is rich source of pharmacologically important glycoside stevioside that is linked to the pathology and complications of diabetes. The current research was carried out to explore the anti-diabetic effect of aqueous extract of Stevia rebaudiana leaves in albino rats. For this purpose, diabetes was induced by administration of streptozotocin (40 mg/kg body weight, intraperitoneally). The diabetic rats were administered with aqueous stevia extract at different dose levels (200, 300, 400 and 500 ppm/kg b.w) for 8 weeks; the control rats were fed basal diet during this period. Stevia aqueous extract improved caloric management and weight control by decreasing the feed intake and body weight gain. Furthermore, intake of stevia extract resulted in significant (P < 0.05) decrease in the random blood glucose level (- 73.24%) and fasting blood glucose (- 66.09%) and glycosylated (HbA1c) hemoglobin (5.32%) while insulin (17.82 ...
The Indonesian Biomedical Journal
BACKGROUND: The prevalence of diabetes mellitus is growing every year, including in Indonesia. Medicinal herbs were used empirically for lowering blood glucose. One of potential herb to have hypoglycemic activity is Stevia rebaudiana Bertoni. S. rebaudiana Bertoni leaves contain stevoside, a natural, lowcalorie sweetener that is 300 times sweeter than saccharose. In this study, we aimed to explore the hypoglycemic activity of S. rebaudiana Bertoni leaves extract in a rat model of type 2 diabetes mellitus (T2DM).METHODS: Male Wistar rats were feed high-fat, highcarbohydrate feed and sugar solution for 74 days to induce a diabetic rat model. The animals were then divided into five groups consisting of a negative control group treated with 2% Pulvis Gom Arabicum; a positive control group treated with Metformin 45 mg/kg body weight; and three test groups treated with aqueous extract of S. rebaudiana Bertoni leaves at doses of 3.125, 6.25 and 12.5 mg/kg BW for 36 days. Blood glucose was ...
American Journal of Biomedical Science & Research , 2019
Abstract Background: Extract of leaves from the Stevia rebaudiana Bertoni have been used in the traditional treatment of diabetes in Paraguay and Brazil. Stevia is a natural, non-caloric sweetener that is rich in pharmacologically important glycosides. These glycosides have many potential benefits in the management of the complications of diabetes. The treatment of type 2 diabetes mellitus (T2DM) is currently unsatisfactory. Therefore, we investigated the possible effects of stevia on treatment of T2DM when concurrently given with common antidiabetic agents in a trial to provide a safe and effective therapeutic antidiabetic combination.Methods: Type 2 diabetes mellitus was induced in albino rats by IP administration of 230 mg/kg of nicotinamide (NA) followed by 65 mg/kg of streptozotocin (STZ). Albino rats were divided into five groups including normoglycemic, diabetic and three diabetic groups in which, the first was treated with aqueous extract of stevia (300 mg/kg), the second was treated with metformin (250 mg/kg), and the third was treated with a combination of metformin and stevia extract with the same doses for the period of 21 days. The rats were dissected; blood samples, liver and kidney were further used for detecting biochemical and histopathological changes. BG, insulin, adiponectin, TG, cholesterol, HDL, ALT, AST, urea, creatinine, total protein and TNFα levels were measured in sera. MDA concentration was detected in the liver and kidney.Results: The aqueous extract of stevia significantly reduced the BG, triglycerides, cholesterol, ALT, AST, urea and creatinine levels in treated rats compared with diabetic rats (p<0.05). In addition to this, stevia surprisingly, increased insulin and adiponectin levels and decrease TNFα Level in treated rats (p<0.05). Stevia extracts also reduced the MDA concentration in the hepatic and renal tissue. Furthermore, stevia compensated for the histopathological damage in diabetic rats. All these changes were more significant when the stevia extract was combined with metformin. Conclusion: It is concluded that the stevia alone and/or in combination with other antidiabetic agents can be a new putative drug used for the treatment of type 2 diabetes mellitus. Moreover, the combination of stevia and metformin has synergistic positive effects on type 2 diabetes mellitus
Applied Biological Chemistry
Stevia (Stevia rebaudiana Bertoni) is a natural zero calorie sweetener with significant economic and medicinal values due to its high contents of steviosides (SVGs) in the leaves. The aqueous extract of Stevia leaves (TAqE) was standardized to contain 8.5% w/w of SVGs (HPLC), total phenolics (164.63 ± 1.39 µg Gallic acid/mg extract) and total flavonoids of 100.5 ± 0.79 µg QE/mg extract. Twenty-one compounds were tentatively identified in the leaves via UPLC-Orbitrap HRMS and stevioside, rebaudioside A, and quercetrin were isolated from TAqE by repeated column chromatography. Stevioside showed significant inhibition of pancreatic lipase, α-amylase, and α-glucosidase enzymes. The effect of a standardized TAqE on high fat diet (HFD)-streptozotocin (STZ)-induced diabetic rats was investigated. Thirty-six animals were divided into 6 groups (each of 6). Rats in group I (control) and group II (control/HFD-STZ) received distilled water, and rats in groups III and IV received TAqE for 4 week...