Infrequent Finding of Metallo- -Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia (original) (raw)
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FIM-1, a New Acquired Metallo- -Lactamase from a Pseudomonas aeruginosa Clinical Isolate from Italy
Antimicrobial Agents and Chemotherapy, 2013
ABSTRACTAcquired metallo-β-lactamases (MBLs) are resistance determinants of increasing clinical importance in Gram-negative bacterial pathogens, which confer a broad-spectrum β-lactam resistance, including carbapenems. Several such enzymes have been described since the 1990s. In the present study, a novel acquired MBL, named FIM-1, was identified and characterized. TheblaFIM-1gene was cloned from a multidrug-resistantPseudomonas aeruginosaclinical isolate (FI-14/157) cultured from a patient with a vascular graft infection in Florence, Italy. The isolate belonged in the sequence type 235 epidemic clonal lineage. The FIM-1 enzyme is a member of subclass B1 and, among acquired MBLs, exhibited the highest similarity (ca. 40% amino acid identity) with NDM-type enzymes. InP. aeruginosaFI-14/157, theblaFIM-1gene was apparently inserted into the chromosome and associated with ISCR19-like elements that were likely involved in the capture and mobilization of this MBL gene. Transfer experiment...
Antimicrobial agents and chemotherapy, 2002
A Pseudomonas aeruginosa isolate highly resistant to carbapenems was collected from a patient with postsurgical cerebrospinal infection in Greece. The isolate carried a class 1 integron that contained as a sole cassette the gene bla(VIM-4), a novel variant of bla(VIM-1), with one nucleotide difference resulting in a Ser-to-Arg change at amino acid position 175 of the VIM-1 enzyme. This is the first detection of a VIM-1 variant after its appearance in Italy.
Background: Acquired carbapenem resistance (R) due to metallo-β-lactamase (MβL) production in P. aeruginosa is increasing rapidly in Asia, Europe and South America. However, it has rarely been reported in North America, with no reports from Mexico. We characterize the MβL-producing P. aeruginosa strains isolated in a Mexican hospital. Methods: A total of 41 clinical isolates collected in 2003 were tested by MicroScan and CLSI agar dilution methods. MβL production was screened by disk approximation test (DA) and MβL Etests strips, while MβL genes and integrons were detected using PCR primers. DNA sequence analysis was done by BLAST and epidemiologic typing was also performed by PFGE. Southern hybridization analysis was performed with blaIMP-1-specific DNA probe. Results: All 41 strains were R to imipenem (IMP) with high rates of R (%) to meropenem (63), Ceftazidime (61), amikacin (51), gentamicin (54) and ciprofloxacin (56). PFGE results showed one major clone with two subtypes (12 i...
Journal of Clinical Microbiology, 2003
Fifty-eight imipenem-nonsusceptible (MIC >or= 8 microg/ml) Pseudomonas aeruginosa strains isolated during May 2001 in 15 Greek hospitals were studied. Thirty-six isolates derived from nine hospitals carried VIM-type metallo-beta-lactamase genes, as found by PCR. In 34 isolates, bla(VIM) was associated with class 1 integrons of various sizes. DNA sequencing indicated the presence of bla(VIM-2) gene cassettes in a variety of integron structures. Random amplified polymorphic DNA typing suggested diversity of the bla(VIM)-positive strains. Synergy between 2-mercaptoacetic acid and imipenem indicated carbapenemase activity in 26 bla(VIM)-positive strains.