Additional file 1: of A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits (original) (raw)

2019

Abstract

Figure S1. Sequencing results for PCRed cI gene. Figure S2 Characterization of wild type (WT) cI and frame-shifted cI. Figure S3 Correlation between the dynamic range and the approximate ratio of plasmid copy number carrying frame-shifted cI and PR-EGFP construct. Figure S4 Open reading frames of wild type cI, frame-shifted cI and truncated cI started from amino acid M41. Figure S5 Generic plasmid maps constructed in this study. Figure S6 Characterization of the NOT gates repression behaviour with postulated truncated variants of cI. Table S1 Curve fitting parameter values. Table S2 Translation initiation rates for EGFP and cI calculated from RBS calculator. Table S3 List of promoters. Table S4 List of plasmids. Table S5 List of primers. (DOCX 4535 kb)

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