Corresponding Author: Efficacy of Composting Dead Poultry and Farms Wastes Infected with Avian Influenza Virus H5N1 (original) (raw)

Abstract

Composting had proven to be an environmentally sound method for disposing dead birds. The composting process management and monitoring it´s thermal profile, moisture content, nutrient ingredients were determined and recorded during day 1 to 33. Isolation and characterization of H5N1 avian influenza virus (AIV) in freshly dead birds and their wastes before, during and after composting was carried out using RT-PCR based assay and sequence analysis. In composting the temperature was increased gradually from 40-60°C through days 5 to 15 then declined after day 15 till end composting. The dry conditions and increased temperature were important virus determinants. Failure of re-isolation of virus in consequence to increased temperature during composting was proven when tested at day 15, end composting and dryness period. AIV was characterized before composting in the birds trachea and compost mix. Positive isolation, characterization and sequence analysis of fragment 4 of H5 gene revealed...

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