Collagen cross-linkers on dentin bonding: Stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition (original) (raw)
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Egyptian Dental Journal
Purpose: To investigate the effect of treatment the demineralized dentin with two naturally derived collagen cross-linking agents on resin-dentin shear bond strength using three clinically relevant treatment times. Materials and methods: 60 mid-coronal dentin specimens were randomly divided into two divisions (n=30) and either was treated with one of two cross-linking agents: 25% grape seed extract (GSE) and 25% ascorbic acid (ASA). The teeth of each division were further divided into three groups (n=10) according to the time of cross-linking agent application: 30 sec, 60 sec, and 90 sec. Additionally, 10 teeth were used as a control group (no treatment). After cross-linkers treatment and adhesive application, composite (Filtek Z250 XT) cylinders (3 mm diameter 2 × mm length) were built on all dentin surfaces. All specimens were stored in distilled water for 24 hours at 37˚C and then subjected to shear stress in a universal testing machine. Failure patterns were observed using a light microscope at 10X. The micromorphology of the fractured surfaces of selected specimens was evaluated using SEM. SBS data (MPa) were statistically analyzed by twoway ANOVA and t-test. Results: Both cross-linking agents resulted in a significant (P<0.0001) increase in resin-dentin SBS in comparison to control, regardless of application time. SBS values of both cross-linking agents were significantly increased by increasing their application time from 30 to 90 sec, while they were not affected, between other treatment times. There was no significant (P = 0.5) differences in SBS values between the two cross linkers at each corresponding treatment time. Conclusions: Resin-dentin SBS can be improved after treatment of demineralized dentin by GSE, and ASA cross-linking agents at a clinically relevant treatment times.
Journal of the Pakistan Medical Association, 2020
Objective: To investigate the effect of natural crosslinkers proanthocyanidin, genipin and glutara ldehyde o n shear bond strengt h at the composite resin-dentin inter face. Method: The in-vitro study was conducted at the Postgraduate Medical Institute, Lahore, Pakistan, from June to September 2018. Exposed dentin surfaces of extracted teeth were conditioned and randomly divided into proanthocyanidin, genipin, glutaraldehyde and control groups according to the type of surface treatment. The dentin surfaces were treated with 6.5% of primers proanthocyanidin, genipin, glutaraldehyde in the relevant groups, while teeth in the control group did not receive any primer application. After thorough rinsing, surfaces of all teeth were restored with a bonding agent and a restorative composite. After 24h, shear bond strength was tested at the Pakistan Council of Scientific and Industrial Research laboratories in Lahore. Pattern of fractures and quality of interface were investigated microscopically at the Lahore campus of COMSATS University, Islamabad. Data was analysed using SPSS 22. Results: Of the 80 teeth, there were 20(25%) in each of the 4 groups. Surface treatment in the three intervention groups significantly raised the shear bond strength at the composite resin-dentin interface compared to the control group (p<0.05). Conclusion: Chemical modification with collagen crosslinkers improved bond strength at the composite resin-dentin interface.
Impact of Various Cross Linking Agents on Dentin Adhesive Systems - an In-vitro Study
Journal of Pharmaceutical Research International, 2021
Aims and Objectives: The main aim of this study is to compare and evaluate the shear bond strength of composite resin using a total etch adhesive and self-etch adhesive after treatment with different collagen cross-linking agents. Materials and Methods: Forty freshly extracted human maxillary central incisors were taken. The proximal dentin was exposed, cavities were prepared on mesial and distal of each tooth. The specimens were randomly divided into two groups based on bonding agent applied-Group A and Group B i.e universal bonding agent and all in one bonding agent respectively and are subdivided into four groups based on the surface treatment of dentin. Group I A (n=10) Control-total etch(CTE); Group II A (n=10) Sodium Ascorbate-total etch (STE); Group III A (n=10)- Proanthocyanidin total etch(PTE); Group IV A (n=10)- Chitosan total etch (CHTE); Group I B (n=10) Control-self etch (CSE); Group II B (n=10) Sodium Ascorbate-self etch(SSE); Group III B (n=10)- Proanthocyanidin self-...
Effect of natural cross-linkers incorporation in a self-etching primer on dentine bond strength
Journal of Dentistry, 2012
The aim of this in vitro study was to investigate the effect of incorporation of natural cross-linkers into the primer of a self-etching adhesive on resin-dentine bond strength. Methods: Flat dentine surfaces were prepared from extracted human molar teeth and were applied with the following self-etching primers. The 0.5% hesperidin (HPN), 0.5% chlorhexidine (CHX) or 0.5% grape seed extract (GSE) was incorporated into Clearfil SE primer (Kuraray Medical, Inc.) to formulate three experimental primers. The original SE primer served as control. Following primer application, the teeth were bonded with Clearfil SE bond, restored with resin composite and stored in water for 24 h at 37 8C. The bonded specimens were sectioned into beams and subjected to micro tensile bond testing (mTBS). Failure analysis and morphological evaluation of the dentine surfaces were performed using a scanning electron microscope (SEM). Hardness (H) and elastic modulus (EM) were measured using nano-indentation technique to examine the mechanical properties of the bonded interfaces. Results: One-way ANOVA showed significant differences in mTBS, H and EM among the tested and control groups (p < 0.001). Tukey post hoc test revealed that incorporation of HPN significantly increased mTBS, H and EM, when compared with the other groups (p < 0.006). The GSE-incorporated group significantly decreased mTBS, H and EM, when compared with the other groups (p < 0.006); while CHX-incorporated group did not show any statistical significant difference when compared with the control group. Conclusion: Incorporation of HPN into Clearfil SE primer had a positive influence on the immediate mTBS and mechanical properties of the bonded interface.
2 3 Effectiveness and biological compatibility of different generations of dentin adhesives
Objectives Besides possessing good mechanical properties, dental materials should present a good biological behavior and should not injure the involved tissues. Bond strength and biocompatibility are both highly significant properties of dentin adhesives. For that matter, these properties of four generations of adhesive systems (Multi-Purpose/Single Bond/SE Plus/Easy Bond) were evaluated. Materials and methods Eighty bovine teeth had their dentin exposed (500-and 200-μm thickness). Adhesive was applied on the dentin layer of each specimen. Following that, the microshearing test was performed for all samples. A dentin barrier test was used for the cytotoxicity evaluation. Cell cultures (SV3NeoB) were collected from testing materials by means of 200-or 500-μm-thick dentin slices and placed in a cell culture perfusion chamber. Cell viability was measured 24 h post-exposition by means of a photometrical test (MTT test). Results The best bonding performance was shown by the single-step adhesive Easy Bond 21 MPa, 200 μm; 27 MPa, 500 μm) followed by Single Bond (15.6 MPa, 200 μm; 23.4 MPa, 500 μm), SE Plus (18.2 MPa, 200 μm; 20 MPa, 500 μm), and Multi-Purpose (15.2 MPa, 200 μm; 17.9 MPa, 500 μm). Regarding the cytotoxicity, Multi-Purpose slightly reduced the cell viability to 92 % (200 μm)/93 % (500 μm). Single Bond was reasonably cytotoxic, reducing cell viability to 71 % (200 μm)/64 % (500 μm). The self-etching adhesive Scotchbond SE decreased cell viability to 85 % (200 μm)/71 % (500 μm).