[Impact of Martian and Lunar Dust Simulants on Cellular Inflammation in Human Skin Wounds ex vivo] (original) (raw)

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Mikrochirurgie der Peripheren Nerven und Gefässe : Organ der Vereinigung der Deutschen Plastischen Chirurgen, 2014

Abstract

In 2006 NASA published its plans to build a manned lunar station in order to undertake missions to Mars in the future. Thus research projects have been conducted on the influence of Lunar and Martian dust on human health. The present study investigated the effect of Lunar and Martian dust simulants (LDS, MDS) in comparison with earth dust (ED) on viability, migration and inflammatory reaction during wound closure in an ex vivo human skin wound model. 6 mm full-thickness skin explants, with a central 3 mm epidermal wound were cultured with LDS, MDS or ED for 4 and 8 days and compared to wound closure without dust exposure. Tissue and conditioned medium were submitted to histological, immunohistochemical (Ki67, Caspase-3) and biochemical analyses (hydroxyproline assay, zymography, IL-6, TNF-α and TGF-β ELISA). All dusts increased proinflammatory markers with significant increases in MDS-treated samples (IL-6: p<0.05; MMP-9: p<0.005) and reduced MMP-2 (p<0.05) compared to no d...

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