Microbial Recovery of Manganese using Staphylococcus Epidermidis</i> (original) (raw)
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Microbial Recovery of Manganese using Staphylococcus Epidermidis
International Journal of Nonferrous Metallurgy, 2012
Manganese minerals are widely distributed throughout the globe. The most important industrial uses of Mn are in the manufacture of steel, non-ferrous alloys, carbon-zinc batteries and some chemical reagents. Microbial recovery of manganese from low grade manganese ores using bioleaching was investigated in this paper. A bacterial strain, Staphylococcus epidermidis (MTCC-435) was collected from microbial type culture collection, IMTECH Chandigarh and used for the experiment. The experimental results for bioleaching with S. epidermidis showed that under pH 5.5, particle size −150 μm, pulp density 10%, temperature 35˚C and agitation 200 rpm, about 80% of Mn was recovered within 20 days of incubation.
A comparative study for Bioleaching of various manganese ores
A native microorganism, Penicillium citrinum was isolated from the top soil of a manganese mine. Based on its efficiency for manganese solubilisation, it was utilized for the leaching of a low-grade manganese ore. The effects of various parameters such as pulp density, particle size, sucrose concentration, inoculum size and bioleaching duration on manganese ore were studied. The optimised conditions for maximum solubilization of the manganese ore (64.58% Mn) were : a particle size of 45µm; a pulp density of 2% (w/v); a sucrose concentration of 10% (w/v); an inoculum dosage of 10% (v/v); and a 30 day duration.
Bioleaching of manganese from low-grade ores using manganese- reducing microbial cultures
Advances in Water Resources, 1999
Ciinihncvr sp rdated iinm \cdirnenr mmplc o f a polluled crcch was found ai rcduci. and lrencc \oluh,l!?e mmganeie i m m niamanese d l o x~l e Thc o~r i m u m cond~t~ana Tor manemrsc ~cducrmn werc. 0k1 (9.51. tempclature (35°C). lnoculum srze (10' ctulml). and plucove (10 811) 'Akn lllc cullurr wab used for lcnchlng 01 manganzac iriim a low g r a k pyrolustt ole in a $Orred lank rexax, >YO% m;ingsne\c could be mlrzslcd 111 13 d q i :a 1% (wh! pulp densiv The p i w x ha.; the potcnnal of hecnnmp an atftc~enl alrcrnativc to the conrentiund proceusci tor Ieachin_o rd low-gradc pyrolu\llc ows 'For conespondcncc
Bioleaching of manganese by Aspergillus sp. isolated from mining deposits
Chemosphere, 2017
A comprehensive study on fungus assisted bioleaching of manganese (Mn) was carried out to demonstrate Mn solubilization of collected low grade ore from mining deposits of Sanindipur, Odisha, India. A native fungal strain MSF 5 was isolated and identified as Aspergillus sp. by Inter Transcribed Spacer (ITS) sequencing. The identified strain revealed an elevated tolerance ability to Mn under varying optimizing conditions like initial pH (2, 3, 4, 5, 6, 7), carbon sources (dextrose, sucrose, fructose and glucose) and pulp density (2%, 3%, 4%, 5% and 6%). Bioleaching studies carried out under optimized conditions of 2% pulp density of Mn ore at pH 6, temperature 37 °C and carbon dosage (dextrose) resulted with 79% Mn recovery from the ore sample within 20 days. SEM-EDX characterization of the ore sample and leach residue was carried out and the micrographs demonstrated porous and coagulated precipitates scattered across the matrix. The corresponding approach of FTIR analysis regulating ...
International Journal of Mineral Processing, 1997
In the present paper a study on bioleaching of manganiferous ores by heterotrophic mixed cultures is reported. The main goal of this work has been to study the bioreduction process of MnO2 in lab-scale reactor and pilot plant tests, in order to investigate the process' pergormances in a scale larger than in shaken flasks. Lab-scale reactor tests were carried out in a batch and in a semi-continuous regime, monitoring manganese extraction yields and microbial growth. In the bioleaching tests 95–100% of manganese extraction (in 36–48 h of treatment using a content of pulp equal to 20% (w/v) of ore having a Mn grade of 17–20%) was obtained. Final tests in a pilot plant (V = 70L) were performed under non-sterilised conditions. From the experimental results a flowsheet of the process has been proposed. An approximate economical analysis of the bioleaching process has also been reported. The experimental results showed the technical feasibility of the process although several problems have to be resolved to allow for a full-scale application, such as the biomass disposal, the purification of the leach liquor before the final manganese recovery and the too high cost of the process.
Biodiversitas Journal of Biological Diversity
Biochar can improve manganese ore extraction during bioleaching by inducing redox reactions and providing a porous matrix for bacterial attachment. In this research, the effect of variations in biochar concentration on the performance of a bioleaching column with a mixed culture of Acidithiobacillus sp. KL3 and Bacillus niacini KB3B1 were studied comprehensively for 30 days. Addition of 0.4% biochar resulted in 89% manganese extraction. Bioleaching with biochar promoted the oxidation of sulfur and yielded high sulfate concentrations, much better pH, and excellent oxidation-reduction potential conditions. The bacteria in the mixed culture survived and adapted to the extreme column environment by releasing extracellular polymeric substance, as evidenced by the sharp increase in the content of the material in the column to 135.7 mg/g over 30 days of incubation. The synergistic effect of the bacteria in the mixed culture greatly contributed to the mechanism of manganese extraction in the column, as indicated by changes in relative bacterial abundance, which is related to bacterial community succession during bioleaching. The relative abundance of B. niacini KB3B1 gradually increased and peaked at 30% over 9 days of measurements. By comparison, the abundance of Acidithiobacillus sp. KL3 first decreased over 9 days of bioleaching and then increased to a maximum of 80% in the final stages of the process. This finding reveals that biochar addition could enhance the mechanisms of bioleaching and improve the yield of manganese extraction.
Biodiversitas Journal of Biological Diversity, 2019
The present research evaluated the manganese bioleaching potency of a heterotrophic bacteria KB3B1. This bacterial strain has been isolated from sulfuric area located at Ungaran, Middle of Java, Indonesia using modified 9K medium by adding of several organic nutrients. The manganese bioleaching activities of the strain was analyzed by applying of varying glycine concentrations (0, 5, 10, 15 mg mL-1) with pyrolusite pulp densities of 0.02 g cm-3 on a rotary shaker at 180 rpm for 18 days incubation. Several parameters, including the growth of bacteria, pH values, the concentration of soluble manganese and cyanide, were investigated at the interval of 3 days. Molecular characteristics of the strain were further analyzed based on 16S rDNA gene sequences. After 15 days, the maximum yield of manganese 16.6% was achieved under the addition of 10 mg mL-1 glycine. This maximum extract obtained was followed by the maximum bacterial growth, pH, and cyanide product of the strain. Phylogenetic analysis showed that the strain was closely related with Bacillus niacini EP89. Besides, the average frequencies of guanine and cytosine (G+C) of the strain was in same range as that of the reference bacteria in the GenBank and Bergey's Manual Systematics of Bacteria.
Bioleaching of manganese (IV) oxide and application to its recovery from ores
Biotechnology Letters, 1990
Bioleaching of manganese (IV) oxide with Thiobacillus thiooxidans has been studied in media with and without sulfur, ferrous sulfide and ferrous sulfate. The knowledge of the role played by the bacteria and the reducing substances suggest that the leaching of manganese (IV) ores through the use of thiobacteria is only justified when suitable amounts of sulfur or metal sulfides are present.
Bioleaching of Low Grade Manganese Ore with Penicillium citrinum
2002
A native microorganism, Penicillium citrinum was isolated from the top soil of a manganese mine. Based on its efficiency for manganese solubilisation, it was utilized for the leaching of a low-grade manganese ore. The effects of various parameters such as pulp density, particle size, sucrose concentration, inoculum size and bioleaching duration on manganese ore were studied. The optimised conditions for maximum solubilization of the manganese ore (64.58% Mn) were : a particle size of 45µm; a pulp density of 2% (w/v); a sucrose concentration of 10% (w/v); an inoculum dosage of 10% (v/v); and a 30 day duration.
This study presents the isolation and screening of manganese (II) oxidizing bacteria from wastewater samples of electroplating industrial effluent and its application as a potential biosorbent to remove Mn(II) ions from aqueous solution in a batch system. A statistical approach, the response surface methodology is used to determine the optimum conditions for the generation of biogenic manganese oxides and manganese removal using manganese oxidizing bacterial strain Mn 21. Based on the statistical analysis; the maximum biogenic manganese oxide formation and manganese removal was obtained 64.90% and 96.90% at pH 8, temperature 300C and 10 days incubation time. We can achieve a maximum removal and Mn oxide formation upto 108.9% and 71.1% respectively at optimal conditions of pH 8.0, temperature 31.70C and incubation time of 9.7 days having maximum desirability. The analysis of variance (ANOVA) of Box–Behnken design showed that the proposed quadratic model fitted experimental data very well with coefficient of correlation r2 to be 0.9821, 0.9744 for manganese removal and manganese oxide formation respectively.