Functionalized Hydrophilic Superparamagnetic Iron Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in Liver Cancer Treatment (original) (raw)
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Journal of Molecular Liquids, 2018
Here, we have studied the colloidal properties of carboxyl-amine functionalized superparamagnetic iron oxide nanoparticles (SPIONs with high saturation magnetization) based ferrofluids and their heating efficacies in magnetic fluid hyperthermia (MFH) via specific absorption rate (SAR)/intrinsic loss power (ILP). Moreover, we have systematically investigated the impact of the following heat influencing factors in MFH: (i) concentrations (0.5-8 mg/ml), (ii) surface coatings (trimesic acid (TMA), pyromellitic acid (PMA), terephthalic acid (TA) and aminoterephthalic acid (ATA)), (iii) applied alternating magnetic fields (AMFs-with amplitudes (H)/frequencies (f))-chosen near to Hergt's biological safety limit, and (iv) dispersion media (biological/non-biological) for using SPIONs in in vitro cancer hyperthermia therapy. SPIONs (particularly decorated with dual-surfactants, i.e., TA-ATA) based aqueous ferrofluids have displayed excellent time-dependent temperature rise even at lower concentrations for the applied AMFs, which resulted in enhanced SAR values ranging from 12.5-200.1 W/g Fe because of their high colloidal stability and enhanced π-π conjugations from the close structural orientations of TA/ATA molecules due to high electrostatic attractions of the respective functional groups (-COOH/NH 2). Moreover, high ILP values of up-to 3.9 nHm 2 /kg (higher than the best commercial ferrofluids) are attained on exposure to magnetic fields below the safety limit. Besides, TA-ATA coated SPIONs dispersed in biological/non-biological media have exhibited better thermal responses as compared to their aqueous counterpart and reached therapeutic temperatures at faster rates due to prominent Neel relaxation mechanisms. The highest SAR value of 276.3 W/g Fe is recorded for TA-ATA coated SPIONs dispersed in triethylene glycol (TEG-with high viscosity), ascribed to the lesser inter-particle interactions from electrostatic repulsions of negative charges among carboxyl/ oxygen molecules from SPIONs/TEG respectively. Moreover, TA-ATA coated SPIONs have induced almost 90% cell death in MCF-7 cancer cells in in vitro MFH studies. Thus, the TA-ATA coated SPIONs based ferrofluids have great potential for in vivo/clinical MFH cancer therapies.
Iron Oxide Based Nanoparticles for Magnetic Hyperthermia Strategies in Biological Applications
European Journal of Inorganic Chemistry, 2015
The use of multifunctional nanoparticles (NPs), usually in the range of 3-100 nm, with their newly discovered propertiessuch as superparamagnetic (SPM) behaviour, enhancement of activity and selectivity in catalytic processes and localised surface plasmon resonance (LSPR)-offers new technical possibilities for biomedical applications such as magnetic hyperthermia (MH), plasmonic photothermal therapy (PPTT) and enhanced magnetic resonance imaging (MRI). In addition, the small size of NPs presents a unique opportunity to interfere, in a highly localised and specific way, with natural processes involving viruses, bacteria or cells and allows interference in the development of complex diseases like many
Nanomaterials, 2021
The use of magnetic nanoparticles in hyperthermia, that is, heating induced by alternating magnetic fields, is gaining interest as a non-invasive, free of side effects technique that can be considered as a co-adjuvant of other cancer treatments. Having sufficient control on the field characteristics, within admissible limits, the focus is presently on the magnetic material. In the present contribution, no attempt has been made of using other composition than superparamagnetic iron oxide nanoparticles (SPION), or of applying surface functionalization, which opens a wider range of choices. We have used a hydrothermal synthesis route that allows preparing SPION nanoparticles in the 40 nm size range, with spherical, cuboidal or rod-like shapes, by minor changes in the synthesis steps. The three kinds of particles (an attempt to produce star-shaped colloids yielded hematite) were demonstrated to have the magnetite (or maghemite) crystallinity. Magnetization cycles showed virtually no hys...
Pharmaceutics
We report the synthesis of magnetite nanoparticles (IOMNPs) using the polyol method performed at elevated temperature (300 °C) and high pressure. The ferromagnetic polyhedral IOMNPs exhibited high saturation magnetizations at room temperature (83 emu/g) and a maximum specific absorption rate (SAR) of 2400 W/gFe in water. The uniform dispersion of IOMNPs in solid matrix led to a monotonous increase of SAR maximum (3600 W/gFe) as the concentration decreased. Cytotoxicity studies on two cell lines (cancer and normal) using Alamar Blues and Neutral Red assays revealed insignificant toxicity of the IOMNPs on the cells up to a concentration of 1000 μg/mL. The cells internalized the IOMNPs inside lysosomes in a dose-dependent manner, with higher amounts of IOMNPs in cancer cells. Intracellular hyperthermia experiments revealed a significant increase in the macroscopic temperatures of the IOMNPs loaded cell suspensions, which depend on the amount of internalized IOMNPs and the alternating m...
Magnetochemistry
Magnetic iron oxide nanoparticles were obtained for the first time via the green chemistry approach, starting from two aqueous extracts of wormwood (Artemisia absinthium L.), both leaf and stems. In order to obtain magnetic nanoparticles suitable for medical purposes, more precisely with hyperthermia inducing features, a synthesis reaction was conducted, both at room temperature (25 °C) and at 80 °C, and with two formulations of the precipitation agent. Both the quality and stability of the synthesized magnetic iron oxide nanoparticles were physiochemically characterized: phase composition (X-ray powder diffraction (XRD)), thermal behavior (thermogravimetry (TG) and differential scanning calorimetry (DSC)), electron microscopy (scanning (SEM) and transmission (TEM)), and magnetic properties (DC and HF-AC). The magnetic investigation of the as-obtained magnetic iron oxide nanoparticles revealed that the synthesis at 80 °C using a mixture of NaOH and NH3(aq) increases their diameter a...
Magnetic nanoparticle-based hyperthermia for cancer treatment
Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznań and Polish Society of Radiation Oncology, 2013
Nanotechnology involves the study of nature at a very small scale, searching new properties and applications. The development of this area of knowledge affects greatly both biotechnology and medicine disciplines. The use of materials at the nanoscale, in particular magnetic nanoparticles, is currently a prominent topic in healthcare and life science. Due to their size-tunable physical and chemical properties, magnetic nanoparticles have demonstrated a wide range of applications ranging from medical diagnosis to treatment. Combining a high saturation magnetization with a properly functionalized surface, magnetic nanoparticles are provided with enhanced functionality that allows them to selectively attach to target cells or tissues and play their therapeutic role in them. In particular, iron oxide nanoparticles are being actively investigated to achieve highly efficient carcinogenic cell destruction through magnetic hyperthermia treatments. Hyperthermia in different approaches has bee...
Bioconjugate Chemistry, 2005
New folate-conjugated superparamagnetic maghemite nanoparticles have been synthesized for the intracellular hyperthermia treatment of solid tumors. These ultradispersed nanosystems have been characterized for their physicochemical properties and tumor cell targeting ability, facilitated by surface modification with folic acid. Preliminary experiments of nanoparticles heating under the influence of an alternating magnetic field at 108 kHz have been also performed. The nanoparticle size, surface charge, and colloidal stability have been assessed in various conditions of ionic strength and pH. The ability of these folate "decorated" maghemite nanoparticles to recognize the folate receptor has been investigated both by surface plasmon resonance and in folate receptor expressing cell lines, using radiolabeled folic acid in competitive binding experiments. The specificity of nanoparticle cellular uptake has been further investigated by transmission electron microscopy after incubation of these nanoparticles in the presence of three cell lines with differing folate receptor expression levels. Qualitative and quantitative determinations of both folate nanoparticles and nontargeted control nanoparticles demonstrated a specific cell internalization of the folate superparamagnetic nanoparticles.
Central European Journal of Chemistry, 2013
In the current work, iron oxide magnetic nanoparticles (MNP’s) were synthesized by thermal decomposition of Fe(acac)3-(iron acetylacetonate) compounds in high-boiling organic solvents containing stabilizing surfactants and examined as possible agents for magnetic hyperthermia treatment, according to their structural, magnetic and heating properties. Three different cancer cell lines (DA3, MCF-7 and HeLa cell lines) were used to assess the suitability of the MNP’s. The experimental results proved that the synthesized MNPs are non-toxic and the uptake efficiency was extremely good. Further, from in vitro hyperthermia results, very fast thermal response was observed (reaching hyperthermia levels in less than 200 s), which minimize the duration of the cell and human body exposure in a high frequency AC external magnetic field.
Journal of Chemical Sciences, 2015
Superparamagnetic iron oxide nanoparticles were synthesized by simple co-precipitation method and modified with different coating agents such as ascorbic acid, hexanoic acid, salicylic acid, L-arginine and L-cysteine. The synthesized nanoparticles were characterized by various techniques such as FT IR, XRD, VSM, SEM, TEM and thermal analysis. Both bare and coated magnetites were of cubic spinel structure and spherical in shape. All the magnetite nanoparticles showed superparamagnetic behaviour with high saturated magnetization. In vitro cytotoxicity test of bare and coated nanoparticles was performed using adenocarcinoma cells, A549. Cell viability of bare and L-arginine coated magnetite nanoparticles showed IC 50 value of 31.2 µg/mL proving the compatibility of nanocarriers when compared to others. Hence, L-arginine coated nanoparticles were used for loading the drug paclitaxel and the observed IC 50 value (7.8 µg/mL) shows its potent anti-proliferative effect against A549 lung cancer cell lines. Thus, it can be speculated that the drug paclitaxel loaded L-arginine coated nanoparticles could be used as an effective drug carrier for the destruction of cancer cells.