Assessments of Antioxidant Content and the Anti-Carcinogenic Effect of Extracts of Solanum Rostratum Dunal in Human Cancer Cells (original) (raw)

Cytotoxic Effect and Antioxidant Activity of Bioassay-guided Fractions from Solanum nigrum Extracts

Tropical Journal of Pharmaceutical Research, 2015

Purpose: To evaluate the cytotoxic effect and antioxidant activity of bioassay-guided fractions from Malaysian species of Solanum nigrum. Methods: Methanol leaf and ethanol fruit extracts of Solanum nigrum were subjected to bioassayguided fractionation using column chromatography. The semi-purified fractions were investigated for their in vitro cytotoxic effect against various cancer cell lines using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5diphenyl-2H-tetrazolium bromide (MTT) assay, and for antioxidant activity using 2, 2-diphenyl-1picryhydrazyl (DPPH) assay. Results: From bioassay-guided fractionation, 13 and 17 fractions were obtained from the methanol leaf and ethanol fruit extracts, respectively. In MTT assay, fractions 1, 2 and 4 from methanol extract showed the highest cytotoxic effect against the cancer cells with IC50 of 13.0 µg/mL at 48 h incubation. For the ethanol extract, fractions 14 and 15 showed the highest cytotoxic effect with IC50 of 12.0 µg/mL against K-562 cells, while fractions 13, 14 and 17 showed IC50 of 13.0 µg/mL against HeLa cells. Doxorubicin hydrochloride and vinblastine sulfate inhibited the cancer cells with IC50 range of 1.3 to 17.0 µg/mL. The highest radical scavenging activity was exhibited by fraction 2 from methanol extract with ED50 value of 0.10 mg/ml, while fraction 15 from ethanol extract showed ED50 of 0.79 mg/mL. Ascorbic acid and α-tocopherol exhibited radical scavenging activity of 95.0 ± 0.01 % (ED50 = 0.05 mg/mL) and 93.0 ± 0.01 % (ED50 = 0.10 mg/mL), respectively. Conclusion: Solanum nigrum leaves and fruits are potential sources of cytotoxic and antioxidant agents.

In-vitro cytotoxic effects of Solanum macranthum fruit. Dunal extract with antioxidant potential

Clinical Phytoscience, 2020

Background The current study undertaken to evaluate antioxidant property of Solanum macranthum fruit methanol extract and its in-vitro cytotoxic effects on breast cancer MDA-MB-231 and mice embryo fibroblast (MEF-L929) cell line. Methods The total alkaloids, phenols and flavonoids content in the methanol extract of S.macranthum fruit were estimated. The antioxidant activity was evaluated by FRAP assay, H2O2 scavenging assay and DPPH assay. Anti-inflammatory protein denaturation inhibition assay was carried out using bovine serum albumin. Anticancer property of the extract was evaluated against breast cancer MDA-MB-231 by MTT assay and comparative study was carried out on normal cells using MEF-L929 cell line. Results Phytochemical estimation revealed that total alkaloid content was measurable high (13.6 g/100 g of extract), where as the total phenolics and flavonoids content was 115.5 mg/GAE and 142.6 mg/QE. Further, the antioxidant effect of methanol extract by H2O2 assay showed co...

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

Oriental Pharmacy and Experimental Medicine, 2008

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2'-azino-bis (3-ethylbenzo-thiazoline-6sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with IC 5 0 5 0 values in the range of 83.30-127.30 µg/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with IC 5 0 value of 80.13 µg/ml. Solanum barbisetum possessing both the activities requires further investigation.