Kinetics of Conformational Sampling in Ubiquitin (original) (raw)

Molecular recognition is pivotal in biological processes, with conformational sampling serving as a mechanism for enzyme-substrate and protein-protein interactions. The study presents methods to accurately measure the rate of conformational interconversion in ubiquitin, narrowing the time scale of internal dynamics to (10 AE 9) ms at 309 K. By using relaxation dispersion and dielectric relaxation spectroscopy, the research reveals the possibility of investigating protein dynamics in a previously hidden time window, enhancing the understanding of protein behavior.