Матеrial Science" "Nonequilibrium Phase Transformations" 11-14 September, 2017, Varna, Bulgaria Nanogold-Reduced Graphene Oxide Hybrid Nanomaterial Based Electrochemical Immunosensor for Brettanomyces Bruxellensis (original) (raw)

Different parameters affecting the preparation of the electrochemical biosensor were optimized and the obtained results are shown in figure 3. Fig. 3 Effect of the amount of nanohybrid (a), incubated antibody (b), time of immobilization (c) and coating with 1% BSA (d) on the normalized amperometric signal of the electrode toward 10 3 CFU/ mL Brett. Eapp = −200 mV, 100% relative current intensity = 460 nA Other parameters affecting the measurement of the yeast were optimized as shown figure 4. Fig. 4 Influence o the time (a), and temperature (b) of incubation of the immunosensor with 10 3 CFU/ mL Brett. Eapp = −200 mV, 100% relative current intensity = 460 nA The analytical performance of nanostructures immunosensor was evaluated under these optimal working conditions. This electrode allowed the amperometric detection of B. bruxellensis in buffered solutions and red wine samples in the range of 10-10 6 CFU/mL and 10 2-10 6 CFU/mL, with detection limits of 8 CFU/mL and 56 CFU/mL, resp...