Identification of acetic acid bacteria isolated in Thailand and assigned to the genus Acetobacter by groEL gene sequence analysis (original) (raw)

2014, Annals of Microbiology

The taxonomic studies on acetic acid bacteria have been mainly carried out on isolates from sources obtained in temperate regions, such as Europe, North America, and Japan. However, few reports have been made on isolates from tropical regions, except for strains of Gluconacetobacter diazotrophicus (Gillis et al.) Yamada et al. 1997 (ϵAcetobacter diazotrophicus Gillis et al. 1989). Moreover, there appear to be no reports on the isolation of strains accommodated in the genus Gluconobacter Asai 1935 from sources of the tropical region. This paper is concerned with the isolation and identification of acetic acid bacteria, especially of strains classified in the genus Gluconobacter from sources collected in Indonesia in the tropical region. Materials and Methods Isolation of acetic acid bacteria from Indonesian sources. The enrichment culture was carried out at pH 3.5 for the isolation of acetic acid bacteria from Indonesian sources. Such sources as flowers, nata de coco, brown sugar, fruits, ragi, and mash of soybean paste were collected in Indonesia and incubated in 7.0 ml of the enrichment culture medium. The medium was composed of 1.0% glucose, 0.5% ethanol, 0.3% acetic acid, 1.5% peptone, 0.8% yeast extract, and 100 ppm of cycloheximide and adjusted to pH 3.5 with