MMP1-1607 polymorphism increases the risk for periapical lesion development through the upregulation MMP-1 expression in association with pro-inflammatory milieu elements (original) (raw)

2016, Journal of Applied Oral Science

I ncreased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective: In this study, we evaluated the milieu elements with MMP-1 mRNA levels in vivo. Material and Methods: MMP1-1607 SNP and the mRNA levels of MMP-1, TNF-, IFN-, IL-17A, IL-21, IL-10, IL-4, IL-9, and FOXp3 were determined via RealTimePCR in DNA/RNA samples from patients presenting periapical granulomas (N=111, for both genotyping and expression analysis) and control subjects (N=214 for genotyping and N=26 for expression analysis). The Shapiro-Wilk, Fisher, Pearson, Chi-square ordinal least squares regression tests were used for data controls, comprising a risk factor for periapical lesions development. MMP-1 mRNA levels were higher in periapical lesions than in healthy periodontal ligament samples, as well as higher in active than in inactive lesions. The polymorphic allele 2G carriers presented a genetic polymorphism and the expression levels of MMP-1. Additionally, the pro-and anti-, TNFas complementary explanatory variables of MMP-1 expression. Conclusion: The MMP1-1607 association with increased MMP-1 mRNA levels in periapical lesions. The MMP-1 expression , IL-21, IL-17A, and IFN-g associated with increased MMP-1 levels in periapical lesions, while IL-10, IL-9, or IL-4 presented an inverse association.