Evaluation of the concentrations of products of nitric oxide oxidation and some antioxidant vitamins in sheep naturally infested with parasites (original) (raw)
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Acta Veterinaria Brno
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E and C, β-carotene and retinol) were determined spectrophotometrically in the blood serum of all goats. The results were expressed as nitrate (µg/ml) 7.25 ± 1.31-4.69 ± 0.32; nitrite (µg/ml) 1.52 ± 0.39-1.64 ± 0.19; vitamin E (mg/100) 0.13 ± 0.05-0.42 ± 0.02; vitamin C (mg/100 ml) 1.49 ± 0.26-1.46 ± 0.15; retinol (g/100 ml) 201.51 ± 15.69-234.081 ± 45.15; β-carotene (g/100 ml) 62.71 ± 7.14-53.95 ± 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.
Acta Veterinaria Brno, 2002
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E and C, β-carotene and retinol) were determined spectrophotometrically in the blood serum of all goats. The results were expressed as nitrate (µg/ml) 7.25 ± 1.31-4.69 ± 0.32; nitrite (µg/ml) 1.52 ± 0.39-1.64 ± 0.19; vitamin E (mg/100) 0.13 ± 0.05-0.42 ± 0.02; vitamin C (mg/100 ml) 1.49 ± 0.26-1.46 ± 0.15; retinol (g/100 ml) 201.51 ± 15.69-234.081 ± 45.15; β-carotene (g/100 ml) 62.71 ± 7.14-53.95 ± 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.
Journal of Equine Veterinary Science, 2009
Equine babesiosis is a tick-borne protozoal disease of horses caused by Theileria equi and Babesia caballi. The disease is endemic in most tropical and subtropical areas. The aim of this paper is to assess the antioxidant status, lipid peroxidation, and oxidation products of nitric oxide (NO) in horses and mules naturally infected with T. equi and B. caballi. East and Southeast Anatolian horses and mules living in rural region of the Eastern border of Turkey were used as the material for this study. These animals are used as pack animal (3-7 years of age). Infected animals were in acute or subacute infection period. In the current study, malondialdehyde (MDA), oxidation products of NO (nitrate and nitrite), serum glutathione (GSH), vitamin E, and retinol levels were analyzed in 58 equids (horse and mule) infected with T. equi and B. caballi as well as in 44 healthy equids. Compared with controls, the level of MDA and nitrate increased significantly (P < .01, P < .05, respectively), whereas GSH concentration and levels of vitamin E decreased significantly (P < .01). There was no significant change in the level of nitrite and retinol between two groups. The results of the current study suggest that in equids infected with T. equi and B. caballi, this alteration in the lipid peroxidation, oxidants, and antioxidants may be related to the host's defenses against parasitic infection and may play a central role in the pathologic conditions associated with babesiosis.
Anti-Oxidant Status for the Oxidative Stress in Blood of Babesia Infested Buffaloes
Blood smears; whole blood and serum samples were taken from both Babesia infested and clinically healthy buffaloes to investigate the antioxidant status and oxidative stress. The data recorded a positive correlation between babesiosis and malondialdehyde (MDA) and a negative correlation with reduced glutathione (GSH), total superoxide dismutase activity (t-SOD), glutathione peroxidase activity (GSH-Px), glutathione reductase activity (GR-ase), glutathione S-transferase activity (GST) and glucose 6-phosphate dehydrogenase activity (G6PD). In addition, a significant (P<0.05) increase in serum aldolase activity, lactate, cortisol and iron were observed. We can conclude that babesiosis can induce a great oxidation and stress in infested animals.
Status of lipid peroxidation and antioxidant enzymes in goats naturally infected with Babesia ovis
Acta Parasitologica, 2012
This study aimed to assess lipid peroxidation and antioxidant enzymes in goats naturally infected with Babesia ovis. Red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 15 goats naturally infected with B. ovis as well as same number of healthy goats. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial 18S rRNA gene sequence of B. ovis. Percentage of parasitemia varied from 0.01 to 1%. The activities of erythrocyte GSH-Px, SOD, CAT and TAC were significantly lower (p<0.05) in the infected goats than in healthy ones. MDA concentration in erythrocytes of infected goats was significantly higher in infected goats than in healthy ones (pš0.05). Severity of parasitemia showed a positive correlation with ...
Veterinary Parasitology, 2014
Please cite this article as: Ciftci, G., Ural, K., Aysul, N., Cenesiz, S., Guzel, M., Pekmezci, D., Sogut, M.Ü.,Investigation of the 8-hydroxy-2 -deoxyguanosine, total antioxidant and nitric oxide levels of serum in dogs infected with Babesia vogeli, Veterinary Parasitology (2014), http://dx.
Turkish Journal of Veterinary & Animal Sciences, 2018
This study was carried out to reveal the importance of procalcitonin, C reactive protein, nitric oxide levels, and adenosine deaminase activity in the diagnosis and prognosis of the disease in naturally infected sheep with Babesia ovis. Thirty sheep diagnosed clinically and parasitologically as having Babesia ovis were allocated to 2 groups. The first group was treated only with imidocarp dipropionate and the second group with imidocarp dipropionate and flunixine meglumin. On the seventh day after treatment, blood samples were collected again from the sheep in the babesiosis-infected group and the treatment responses were assessed. Serum PCT (1.72 ± 0.34 ng/mL, P < 0.01), CRP (101.42 ± 11.73 µg/mL, P < 0.001), NO (15.77 ± 2.75 µmol/L, P < 0.01), and ADA (13.92 ± 0.88 IU/L, P < 0.01) were higher in sheep with babesiosis than in the healthy sheep (0.49 ± 0.04 ng/mL, 49.46 ± 4.57 µg/mL, 8.15 ± 0.63 µmol/L, 9.34 ± 1.19 IU/L, respectively). When PCT, CRP, NO, and ADA before treatment and after treatment in the infected sheep were compared, the levels of these parameters except for ADA in the second group were determined to have statistically decreased after the treatment. As a result, it has been concluded that the measurements of PCT, CRP, NO, and ADA in sheep with babesiosis may be useful for the diagnosis and prognosis of the disease when assessed in association with clinical examination.