Evaluation of in Vitro Anti-Urolithiatic Activity of Methanolic Extract of Cucumis Melo Seeds on Calcium Oxalate Crystals (original) (raw)
Related papers
Heliyon
Cucumis callosus dry fruits are traditionally used as folk remedy to treat conditions like urethral irritations, urine stoppage or dribbling and other urinary ailments of man in northwest India. But no study is reported to validate this ethnic practice of using Cucumis fruit in urolithiasis. To evaluate anti-urolithiatic potential of Cucumis, hyperoxaluria was induced in rats by supplying 0.75% ethylene glycol (EG) + 1% ammonium chloride (AC) in drinking water for 14 days. Antiurolithiatic activity of Cucumis callosus hydro-ethanolic extract (CCHEE) was assessed by measuring blood and urine biochemical parameters, oxidative stress indices, histopathology and osteopontin (OPN) expression. Administration of EG-AC to rats caused hyperoxaluria, crystalluria, azotaemia, oxidant/antioxidant imbalance (increase in lipid peroxidation (LPO), and decrease in glutathione (GSH) and catalase (CAT)), up-regulation of OPN and calcium oxalate (CaOx) crystal deposition in kidney. Treatment of afflicted rats with Cucumis fruits extract restored renal function to a great extent (CCHEE group), testified by improvement of stated parameters. Findings demonstrate curative efficacy of Cucumis fruit extract in EG induced urolithiasis of rats. The restoration of renal function was possibly by regulating renal stone formation via reducing urinary oxalate excretion, correcting oxidant/antioxidant imbalances, and reduced expression of OPN. Hence, results of this study validate the ethnic practice of using Cucumis fruit and conclude that fruit extracts have beneficial effects on CaOx urolithiasis and renal function.
Effect of indigenous plant extracts on calcium oxalate crystallization having a role in urolithiasis
Urological Research, 2011
Crystallization process has a major role in urolithiasis. In the present study, eVect of two indigenous plants extracts namely Boerhavia diVusa and Bryophyllum pinnatum extract was determined on the crystallization of calcium oxalate crystals. EVect on the number, size and type of calcium oxalate crystals was observed. Results showed signiWcant activity of both extracts against calcium oxalate crystallization at diVerent concentrations (P < 0.05). Size of the crystals gradually reduced with the increasing concentration of both extracts. The number of calcium oxalate monohydrate crystals which are injurious to epithelial cells gradually reduced and at the highest concentration of extracts (100 mg/ml) completely disappeared (P < 0.05). These results conWrm that B. diVusa and B. pinnatum extracts have antiurolithic activity and have the ability to reduce crystal size as well as to promote the formation of calcium oxalate dihydrate (COD) crystals rather than monohydrate (COM) crystals. Control of crystal size and formation of COD rather than COM crystals, in combination with the diuretic action of extracts is an important way to control urolithiasis.
https://www.ijhsr.org/IJHSR\_Vol.12\_Issue.9\_Sep2022/IJHSR-Abstract21.html, 2022
Many novel compounds had obtained from the plants having medicinal property. Among the medicinal plants, those having antiurolithiatic properties are important and are very effective in controlling and curing of stone formation in the urinary tract. The inhibitory effect of aqueous methanolic extract of traditionally used medicinal plants were studied on the formation of calcium oxalate (CaOX) stone in aqueous as well as in urinary medium. The inhibitory effects were presented as the percentage of inhibition or the degree of inhibition as compared to blank urine. The inhibition in stone formation is due to its capacity of increasing the solubility product of its constituents and is determined through chemoinhibition experiment. The antiurolithiatic properties of different medicinal plants were studied and presented in decreasing order in the present work. The finding give support the effectiveness of many medicinal plants against the urolithiatic cases specially in CaOX formation as used in the folklore medicine.
Asian Journal of Pharmaceutical and Clinical Research
Objective: Ocimum basilicum (OB) has been used to treat diverse illnesses which include urinary stone disorder for a reason that historical time in India. We investigated OB seeds for antiurolithic activity. Methods: Calcium oxalate crystallization becomes triggered by the addition of 0.01 M sodium oxalate answers in normal human urine and nucleation was done. Results: OB seeds were discovered to be robust and promising antiurolithiatic agents which are in accordance with its use in traditional medication. Conclusion: An extract of the traditional herb OB has super inhibitory activity on crystalluria and therefore might be useful in dissolving urinary stone; however, in addition, a study in animal fashions of urolithiasis is needed to assess its capability antiurolithiatic interest.
BMC Complementary Medicine and Therapies
Background The banana or scientifically referred to as Musa sp., is one of the most popular fruits all over the world. Almost all parts of a banana tree, including the fruits, stem juice, and flowers are commonly used as traditional medicine for treating diarrhoea (unripe), menorrhagia, diabetes, dysentery, and antiulcerogenic, hypoglycemic, antilithic, hypolipidemic conditions, plus antioxidant actions, inflammation, pains and even snakebites. The study carried out was to evaluate in vitro anti-urolithiatic activity from different types of Musa pseudo-stems. Methods Observing anti-urolithiathic activity via in vitro nucleation and aggregation assay using a spectrophotometer followed by microscopic observation. A total of 12 methanolic extracts were tested to determine the potential extracts in anti-urolithiasis activities. Cystone was used as a positive control. Results The results manifested an inhibition of nucleation activity (0.11 ± 2.32% to 55.39 ± 1.01%) and an aggregation ac...
IJPSR, 2021
Urolithiasis is a common urological disorder responsible for serious human affliction and cost to society with a high recurrence rate. The aim of the present study was to systematically evaluate the leaf extract of Citrus medica using suitable in-vitro and in-vivo models to provide scientific evidence for its antilithiatic activity. Cystone has been suggested to be beneficial in urolithiasis, as it corrects the crystalloid, colloid stability. It is evaluated that flavonoids, triterpenoids and saponins from different plants resulted in the antilithiatic activity. To explore the effect of Citrus medica on sodium oxalate crystallization, in-vitro assays like crystal nucleation and aggregation were performed. The biochemical parameters like calcium, oxalate, magnesium, phosphate, sodium, and potassium were evaluated in urine, serum, and kidney homogenates. Histopathological studies were also done to confirm the biochemical findings. In-vitro experiments with Citrus medica showed concentration-dependent inhibition of sodium oxalate nucleation and aggregation. In the in-vivo model, Citrus medica reduced both sodium and oxalate supersaturation in urine, serum, and deposition in the kidney. The biochemical results were supported by histopathological studies. The findings of the present study suggest that Citrus medica has the ability to prevent nucleation and aggregation growth of sodium oxalate crystals. Citrus medica has a better preventive effect on sodium oxalate stone formation, indicating its strong potential to develop as a therapeutic option to prevent recurrence of urolithiasis.
Evaluation of In vitro anti urolithiasis activity of herbal drugs
World Journal of Biology Pharmacy and Health Sciences, 2022
Urinary stone disorder has afflicted human kind since antiquity and can persist, with serious medical consequences, throughout a patient's lifetime. The common component of urinary stone is calcium oxalate (CaOx). In spite of tremendous advances in the field of medicine, there is no truly satisfactory drug for the treatment of renal calculi. In the indigenous system of medicine, the leaves Mentha piperita (family-Labiatae) Elettaria cardamom (zingiberaceae) and Syzygium cumini (family-Myrtaceae) are reported to be useful in the treatment of urinary stones. Hence, in the present study, the Mentha piperita, Elettaria cardamom and Syzygium cumini have been selected for their In vitro Antiurolithiatic activity on experimental kidney stones of calcium oxalate crystals.
International Journal of Minor Fruits, Medicinal and Aromatic Plants
The purpose of this study is to evaluate in vitro the effect of an aqueous extract of Urtica dioica on the dissolution of oxalocalcic type kidney stones at the mesoscopic scale. The weight of the stones used in our experiment varied from: 0.0625g to 1.1049g. Type identification of kidney stone samples is performed by Infrared spectroscopic analysis. The presence of carboxylate ion of calcium oxalate is highlighted by absorption bands in the 1312.41 cm-1 and 1606.36 cm-1 areas. The aqueous extract of the aerial part of the plant Urtica dioica was prepared by infusion for 30 min of 5g of powder in 100 ml of saline solution (9 g/L of NaCl), previously brought to the boiling point, and there was then filtered. The stones were left in contact with the extract for 6 weeks, under constant magnetic stirring in 50 ml of aqueous extract. Kinetic evolution of the pH and the evaluation of the dissolution capacity of the extracts were carried out every week. The results obtained are very satisfactory where we observe a loss of mass which increases with time in order to reach a rate of 63%. This confirms the dissolution of stones and the increase of pH by the effect of the presence of the base of calcium oxalate in the aqueous medium. According to this study, we emphasize the need to suggest Urtica dioica as a means to reduce the occurrence of this urological disease and to establish less expensive tests and treatments.
Brazilian Journal of Biology, 2024
The potential of Alhagi maurorum (Boiss.) aqueous extract (AME), used in traditional medicine for treatment or prevention of urolithiasis, to dissolve calcium oxalate stones in vitro was evaluated. In order to determine the litholytic potential of the extract, Calcium oxalate urinary stones were incubated during 12 weeks under continuous shaking in the presence of AME, Rowanix or NaCl 9 g/mL solution were used as controls. After the incubation period, the residual weight of the treated calculi was determined and the rate of dissolution was calculated. The medium pH variation was measured and changes in the calcium oxalate crystals at the stone surface were assessed using a scanning electron microscope (SEM). The results showed a significant dissolution effect for the extract on the kidney calculi during the experimentation period. At the end of the experiment, the percentages of calculi weight decrease were 41.23, 4.97 and 55.67% for the extract, NaCl solution and Rowanix, respectively. Gas Chromatography analysis revealed mainly the presence of the following phyto-compounds: Cyclopropenone, 2,3-diphenyl; 1-Nonadecanol; methyl-alpha-D-mannopyranoside; cis-9-Hexadecenal. These compounds unarguably play crucial roles in the health care system especially in cancer treatment and many other diseases including urolithiasis. The urinary stone dissolution, independent of medium pH, could be attributed to formation of complexes between the phytochemical compounds in the extract and the calculi.